Circular RNA circ_0020123 promotes non-small cell lung cancer progression by acting as a ceRNA for miR-488–3p to regulate ADAM9 expression

Wan, Jingru; Hao, Liguo; Zheng, Xiaoyang; Li, Zheng

doi:10.1016/j.bbrc.2019.05.158

Cited by 56 publications

(47 citation statements)

References 23 publications

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“…ADAM9 was demonstrated to be a target of miR-488-3p in nonsmall cell lung cancer. 26 We also confirmed that miR-488-3p could regulate ADAM9 expression in PTC cells. Therefore, F I G U R E 6 Cancer susceptibility candidate 9 (CASC9) regulates ADAM9 expression by sponging miR-488-3p in papillary thyroid cancer (PTC).…”

Section: Discussionsupporting

confidence: 71%

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Long noncoding RNA CASC9 promotes the proliferation and metastasis of papillary thyroid cancer via sponging miR‐488‐3p

Chen

Gao³

2020

Cancer Medicine

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Cancer susceptibility candidate 9 (CASC9) is a recently identified lncRNA that acted as a tumor promotor in diversified cancer types. However, its role in papillary thyroid cancer (PTC) remains unknown. The expression of CASC9 was measured in 52 human PTC tissues and PTC cell lines as well as their controls. The proliferation, migration, and invasion of PTC cells were determined after knockdown or overexpression of CASC9 to evaluate the effect of CASC9 on PTC cells. Also, the role of PTC tumorigenesis was confirmed in mice xenograft models. Additionally, the underlying mechanisms of CASC9 were further researched. We found that CASC9 expression was augmented in human PTC tissues and cells. Higher CASC9 expression was associated with large tumor size, advanced stage, or lymph node metastasis. Downregulation of CASC9 significantly attenuated the proliferative, migrative, and invasive abilities of PTC cells, and suppressed tumorigenesis in vivo. While overexpression of CASC9 elevated the proliferation, migration, and invasion of PTC cells. miR‐488‐3p expression was decreased, and ADAM9 level was increased in PTC tissues and cells. CASC9 expression was negatively related to miR‐488‐3p, but positively associated with ADAM9 expression in PTC tissues. Molecular mechanism analysis revealed that CASC9 functioned via sponging miR‐488‐3p to regulate ADAM9 expression, followed by activation of EGFR‐Akt signaling. In conclusion, lncRNA CASC9 promoted the malignant phenotypes of PTC via modulating miR‐488‐3p/ADAM9 pathway. This study may provide a novel therapeutic target for the treatment of PTC.

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Section: Discussionsupporting

confidence: 71%

“…This study has shown that miR-488-3p could regulate ADAM9 expression. 26 ADAM9 has been found to be responsible for the migration and invasion of thyroid cancer. 27 Hence, we further verified the relationship between CASC9 and ADAM9 in PTC.…”

Section: Casc9 Regulates Adam9-egfr-akt Signaling By Sponging Mir-4mentioning

confidence: 99%

Long noncoding RNA CASC9 promotes the proliferation and metastasis of papillary thyroid cancer via sponging miR‐488‐3p

Chen

Gao³

2020

Cancer Medicine

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“…Yang et al implicated that miR-488-3p was lowly expressed in esophageal squamous cell carcinoma (ESCC) and its overexpression suppressed ESCC cell growth and promoted apoptosis by targeting ZBTB2 [24]. Wan et al manifested that circ_0020123 contributed to cell growth and motility and impeded apoptosis in NSCLC cells via miR-488-3p/ADAM9 axis [17]. Furthermore, in CRC, Yao et al unraveled that miR-488 was reduced in CRC and its inhibition could target PHF8 to increase CRC cell growth and migration [25].…”

Section: Discussionmentioning

confidence: 99%

“…MiR-495 repressed cell growth and motility and promoted apoptosis in CRC cells via targeting FAM84D [14]. MiR-488-3p acted as an anti-tumor regulator in some human cancers, such as glioma [15], retinoblastoma [16] and non-small cell lung cancer (NSCLC) [17]. Nevertheless, the effects of miR-488-3p in CRC are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

CircSEC24A promotes colorectal cancer progression by regulating miR-488-3p/TMEM106B axis

Hu¹,

Peng²,

Cao³

2020

Preprint

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Background: Currently, more and more circular RNAs (circRNAs) have been identified to exert their functions in tumor progression, including colorectal cancer (CRC). However, the role of circSEC24A (circ_0003528) in CRC remains unknown.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the levels of circSEC24A, SEC24A and microRNA-488-3p (miR-488-3p). The characterization of circSEC24A was investigated by Actinomycin D and RNase R digestion assays. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to assess cell proliferation. Flow cytometry analysis was adopted for cell apoptosis and cell cycle process. Transwell assay was employed to evaluate cell migration and invasion. Western blot assay was performed to determine protein levels. Dual-luciferase reporter assay was utilized to explore the relationship between miR-488-3p and circSEC24A or transmembrane protein 106B (TMEM106B). Murine xenograft model was constructed to explore the effect of circSEC24A in vivo .Results: CircSEC24A level was increased in CRC tissues and cells. CircSEC24A deficiency impeded cell proliferation, cell cycle process, migration and invasion and induced apoptosis in CRC cells in vitro and blocked tumorigenesis in vivo . MiR-488-3p was a target of circSEC24A and miR-488-3p was downregulated in CRC tissues and cells. The inhibitory effect of circSEC24A silencing on CRC cell progression was restored by miR-488-3p inhibition. Moreover, TMEM106B could be negatively regulated by miR-488-3p via acting as a downstream gene of miR-488-3p. MiR-488-3p overexpression decelerated CRC cell progression by targeting TMEM106B.Conclusion: CircSEC24A facilitated CRC progression by regulating miR-488-3p/TMEM106B axis, which might provide a promising treatment approach for CRC.

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“…39 In line with this, other circRNAs, such as circP4HB, circMTO1, circ_0026134, circ-FOXM1, circ_003645, circ_0006427, circABCB10, circ-BANP, circFADS2, circ_103809, circMAN2B2, circ_0012673 and circ_0020123 showed similar roles that were served as sponge of miRNAs to regulate the occurrence and development of lung cancer. [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Emerging evidences demonstrated that cancerassociated chromosomal translocations and encoding fusion gene could generate circRNA, contributing to tumorigenesis. 55 For instance, SLC34A2-ROS1 fusion gene could produce circRNA F-circSR, which promoted cell migration of NSCLC cells.…”

Section: Acting As Autophagy Regulatormentioning

confidence: 99%

<p>Biological Roles and Mechanisms of Circular RNA in Human Cancers</p>

Tang

Hann

2020

OTT

136

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Circular RNA (circRNA) is an intriguing class of RNA with covalently closedloop structure and is highly stable and conservative. As new members of the ncRNAs, the function, mechanism, potential diagnostic biomarker, and therapeutic target have raised increased attention. Most circRNAs are presented with characteristics of abundance, stability, conservatism, and often exhibiting tissue/developmental-stage-specific manner. Over 30,000 circRNAs have been identified with their unique structures to maintain stability more easily than linear RNAs. An increased numbers of circRNAs are dysregulated and involved in several biological processes of malignance, such as tumorigenesis, growth, invasion, metastasis, apoptosis, and vascularization. Emerging evidence suggests that circRNAs play important roles by acting as miRNA sponge or protein scaffolding, autophagy regulators, and interacting with RNA-binding protein (RBP), which may potentially serve as a novel promising biomarker for prevention, diagnosis and therapeutic target for treatment of human cancer with great significance either in scientific research or clinic arena. This review introduces concept, major features of circRNAs, and mainly describes the major biological functions and clinical relevance of circRNAs, as well as expressions and regulatory mechanisms in various types of human cancer, including pathogenesis, mode of action, potential target, signaling regulatory pathways, drug resistance, and therapeutic biomarkers. All of which provide evidence for the potential utilities of circRNAs in the diagnosis and treatment of cancer.

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Circular RNA circ_0020123 promotes non-small cell lung cancer progression by acting as a ceRNA for miR-488–3p to regulate ADAM9 expression

Cited by 56 publications

References 23 publications

Long noncoding RNA CASC9 promotes the proliferation and metastasis of papillary thyroid cancer via sponging miR‐488‐3p

Long noncoding RNA CASC9 promotes the proliferation and metastasis of papillary thyroid cancer via sponging miR‐488‐3p

CircSEC24A promotes colorectal cancer progression by regulating miR-488-3p/TMEM106B axis

<p>Biological Roles and Mechanisms of Circular RNA in Human Cancers</p>

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