Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Xie, Hong; Wang, Jian; Wang, Baiqi

doi:10.2147/cmar.s311242

Cited by 25 publications

(14 citation statements)

References 38 publications

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“…We attempted to grasp the potential molecular mechanism of circ_0005615 during CC progression. Online bioinformatics tool starbase 3.0 (http://starbase.sysu.EDU.cn/) was used to predict the possible miRNA targets of circ_0005615, and we selected eight miRNAs as the follow-up subjects due to their antitumor role in CC progression, including miR-149-5p, [13] miR-758-3p, [14] miR-205-5p, [15] miR-506, [16] miR-143-3p, [17] miR-138-5p, [18] miR-665, [19] and miR-331-3p. [20] RNA-pull down assay showed that miR-506, miR-138-5p, and miR-665 could be pulled down by circ_0005615 probe, especially miR-138-5p (Figure S2A).…”

Section: Circ_0005615 Functioned As Mir-138-5p Spongementioning

confidence: 99%

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR‐138‐5p/KDM2A axis

Luo,

Liu,

Wang

et al. 2023

J Biochem & Molecular Tox

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Cervical cancer (CC) is a highly fatal gynecological malignancy due to its high metastasis and recurrence rate. Circular RNA (circRNA) has been regarded as a regulator of CC. However, the underlying molecular mechanism of circ_0005615 in CC remains unclear. The levels of circ_0005615, miR‐138‐5p, and lysine demethylase 2A (KDM2A) were measured using qRT‐PCR or western blot. Cell proliferation was assessed by Cell Counting Kit‐8, 5‐ethynyl‐2′‐deoxyuridine, and colony formation experiments. Cell invasion and migration were tested by transwell assay and wound healing assay. Flow cytometry and Caspase‐Glo 3/7 Assay kit were used to analyze cell apoptosis. The expression of proliferation‐related and apoptosis‐related markers was detected by western blot. The binding relationships among circ_0005615, miR‐138‐5p, and KDM2A were verified by dual‐luciferase reporter assay or RNA immunoprecipitation assay. Xenograft assay was applied to detect the effect of circ_0005615 in vivo. Circ_0005615 and KDM2A were upregulated, while miR‐138‐5p was downregulated in CC tissues and cells. Circ_0005615 knockdown retarded cell proliferation, migration, and invasion, while promoting apoptosis. Besides, circ_0005615 sponged miR‐138‐5p, and miR‐138‐5p could target KDM2A. miR‐138‐5p inhibitor reversed the regulation of circ_0005615 knockdown on CC cell growth and metastasis, and KDM2A overexpression also abolished the inhibitory effect of miR‐138‐5p on CC cell growth and metastasis. In addition, we also discovered that circ_0005615 silencing inhibited CC tumor growth in vivo. Circ_0005615 acted as a tumor promoter in CC by regulating the miR‐138‐5p/KDM2A pathway.

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Section: Circ_0005615 Functioned As Mir-138-5p Spongementioning

confidence: 99%

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR‐138‐5p/KDM2A axis

Luo,

Liu,

Wang

et al. 2023

J Biochem & Molecular Tox

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“…Studies have shown that circRNAs can regulate the proliferation, metastasis, apoptosis and autophagy of various cancer cells by acting as ceRNAs 10,11 . For example, circ_0003221 accelerated cervical cancer progression by promoting CPEB4 expression through sponging miR‐758‐3p 12 . Meanwhile, circ_001422 could promote osteosarcoma progression through miR‐195‐5p/FGF2 axis 13 .…”

Section: Introductionmentioning

confidence: 99%

“…10,11 For example, circ_0003221 accelerated cervical cancer progression by promoting CPEB4 expression through sponging miR-758-3p. 12 Meanwhile, circ_001422 could promote osteosarcoma progression through miR-195-5p/FGF2 axis. 13 Cir-cABCC1 is located at chr16: 16101672-16162159, and is reverse spliced through linear RNA ATP binding cassette subfamily C member 1 (ABCC1) (http://circbase.org/cgi-bin/simplesearch.cgi).…”

Section: Introductionmentioning

confidence: 99%

Silencing circular RNA hsa_circABCC1 inhibits osteosarcoma progression through down‐regulating HDAC4 via sponging miR‐591

Wang

Liu

et al. 2023

Environmental Toxicology

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Background Circular RNA (circRNA) has been shown to play an important regulatory role in the development of various cancers, including osteosarcoma (OS). However, the role of circRNA ABCC1 (circABCC1) in OS was still poorly understood. The aim of our study was to investigate the role of circABCC1 in OS progression and its potential molecular mechanisms. Methods The expression of circABCC1, microRNA‐591 (miR‐591) and histone deacetylase 4 (HDAC4) in OS tissues or cells was detected by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot (WB) analyses. In vitro experiments, the viability, proliferation, apoptosis, migration, invasion and autophagy of U2OS and HOS cells were assessed in vitro using cell counting kit‐8 (CCK‐8) assay, 5‐ethynyl‐29‐deoxyuridine (EdU) assay, flow cytometry (FCM) assay, transwell migration and invasion assays (transwell) and WB assay, respectively. Interactions between circABCC1 and miR‐591, miR‐591 and HDAC4 were confirmed using a dual luciferase reporter gene assay system. The oncogenic role of circABCC1 in OS in vivo was examined by establishing a tumor xenograft model. Results CircABCC1 was significantly elevated in OS tissues (about 3.1‐folds) and cells (U2OS (about 2.1‐folds) and HOS (about 2.8‐folds)) compared with the control (p < .05). Silencing of circABCC1 significantly reduced the viability and proliferation, promoted apoptosis, impaired migration and invasion, and increased autophagy of U2OS and HOS cells (p < .05). In addition, miR‐591 was confirmed to be a target of circABCC1, exerting an opposite effect to circABCC1 (p < .05). MiR‐591 attenuation in U2OS and HOS cells was able to reply to the inhibition of cell proliferation, migration and invasion as well as promotion of cell apoptosis and autophagy mediated by silencing circABCC1 (p < .05). HDAC4 was verified to be the target gene of miR‐591 in U2OS and HOS cells and was regulated by the circABCC1/miR‐591 axis (p < .05), and restoration of HDAC4 levels in U2OS and HOS cells was able to restore the altered cellular function caused by silencing circABCC1 (p < .05). In addition, knockdown of circABCC1 attenuated tumor growth in vivo (p < .05). Conclusion Silencing of circABCC1 inhibits osteosarcoma progression by attenuating HDAC4 expression through sponging miR‐591.

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“…CircRNAs act as cancer regulators and novel biomarkers through sponging microRNAs (miRNAs) to affect gene expression [ 9 ]. Circ_0003221 accelerated cell malignant phenotypes of cervical cancer via regulating cytoplasmic polyadenylation element-binding protein 4 by playing the sponge effect on miR-758-3p [ 10 ]. Also, circ_0003221 was displayed to lead to the promotion of BLCA cell migration and proliferation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0003221 facilitates the malignant development of bladder cancer cells via resulting in the upregulation of DHCR24 by targeting miR-892b

Jiang

Xia

2022

Investig Clin Urol

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Purpose This research concentrated on the biological effects and special mechanism of circ_0003221 in bladder cancer (BLCA). Materials and Methods The level quantification by reverse transcription-quantitative polymerase chain reaction was administrated for circ_0003221, microRNA-892b (miR-892b) and 24-dehydrocholesterol reductase (DHCR24). The biological behaviors were assessed by EDU assay and colony formation assay for proliferation, and transwell assay for cell motility. Glycolytic metabolism was tested using the commercial kits. DHCR24 protein level and cell markers were measured through western blot. The analysis of interaction potential was conducted via dual-luciferase reporter assay and pull-down assay. Circ_0003221 was implemented via tumor xenograft assay in vivo . Results Abnormal circ_0003221 upregulation was affirmed in BLCA. BLCA cell proliferation, motility and glycolysis were impeded after circ_0003221 level was knocked down. MiR-892b was identified as a target for circ_0003221. Reduction of miR-892b relieved si-circ_0003221-induced anti-tumor response in BLCA cells. In addition, miR-892b targeted DHCR24 and circ_0003221/miR-892b could regulate the level of DHCR24. The effects of si-circ_0003221 were also counteracted by DHCR24 overexpression. Conclusions The current evidence elucidated circ_0003221 targeted miR-892b to elevate the DHCR24 level, thus accelerating cell development and glycolytic metabolism of BLCA cells.

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Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Cited by 25 publications

References 38 publications

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR‐138‐5p/KDM2A axis

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR‐138‐5p/KDM2A axis

Silencing circular RNA hsa_circABCC1 inhibits osteosarcoma progression through down‐regulating HDAC4 via sponging miR‐591

Hsa_circ_0003221 facilitates the malignant development of bladder cancer cells via resulting in the upregulation of DHCR24 by targeting miR-892b

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