Circular RNA circ_0001287 inhibits the proliferation, metastasis, and radiosensitivity of non-small cell lung cancer cells by sponging microRNA miR-21 and up-regulating phosphatase and tensin homolog expression

Abstract

As a type of non-coding RNA, circular RNA (circRNA) figures prominently in human cancer progression. Nonetheless, the expression, function, and regulatory mechanism of circ_0001287 in non-small cell lung cancer (NSCLC) remain obscure. In this work, quantitative real-time polymerase chain reaction (qRT-PCR) was implemented to quantify circ_0001287 and miR-21 expressions in NSCLC tissues and cells. The relationship between circ_0001287 expression and the clinicopathological parameters of NSCLC patients was examined. Cell counting kit-8 (CCK-8), 5-bromo-2©-deoxyuridine (BrdU), and Transwell experiments were conducted to detect the multiplication, migration, and invasion of NSCLC cells after circ_0001287 was overexpressed or knocked down. The survival of NSCLC cells was studied using colony formation experiment under different doses of radiation. RNA immunoprecipitation (RIP) experiment and luciferase reporter gene experiment verified the binding relationship between circ_0001287 and miR-21. Western blot was employed to examine the regulatory effects of circ_0001287 and miR-21 on phosphatase and tensin homolog (PTEN) expression. We reported that circ_0001287 expression was down-modulated in NSCLC tissues and cell lines. Besides, circ_0001287 low expression was associated with low differentiation and positive lymph node invasion of NSCLC. Circ_0001287 overexpression suppressed the multiplication, migration, invasion, and radioresistance of NSCLC cells, whereas circ_0001287 knockdown promoted the above phenotypes. Circ_0001287 could adsorb miR-21 and repress its expression, and indirectly up-modulate PTEN expression in NSCLC cells. Taken together, circ_0001287/miR-21/PTEN axis is probably involved in regulating NSCLC cell multiplication, metastasis, and radioresistance.

Biogenesis, Functions, and Role of CircRNAs in Lung Cancer

“…For example, circ_0001287 can reduce radiotherapy resistance, thus providing new clues for the treatment of lung cancer. 136 Therefore, circRNAs are closely related to the solution of radiotherapy resistance.…”

CircPTCH1 Promotes Migration in Lung Cancer by Regulating MYCN Expression Through miR-34c-5p

“…More and more studies have shown that non-coding RNA plays a key role in a variety of human diseases, such as circRNA can directly participate in the regulation of cell proliferation, differentiation, migration and invasion by activating a variety of signaling pathways, thus playing an important role in the occurrence and development of malignant tumors. [8][9][10][11][12][13] and a variety of circular RNAs play an important regulatory role in the proliferation, invasion and metastasis of lung cancer cells. [14][15][16] Studies have shown that circPTCH1 is related to the occurrence and development of tumor, participates in the formation, invasion and metastasis of tumor, and may play the role of oncogene in tumor, 17 but the mechanism of circPTCH1 in lung cancer is still unclear.…”

The expression profile and clinical application value of hsa_circ_0016148 in head and neck squamous cell carcinoma

“… 13 , 15 One recent study by Zhang and his colleagues showed that circular RNA circ_0001287 inhibited the proliferation, metastasis, and radiosensitivity of non‐small cell lung cancer cells by sponging miR‐21. 16 Hao et al reported that circDCUN1D4 suppressed tumor metastasis and glycolysis in lung adenocarcinoma by stabilizing TXNIP expression. Additionally, due to its characteristics of abundance, diversity, stability, and disease‐specific exhibition, circRNA was considered as ideal biomarker for auxiliary diagnosis and prognosis estimation of disease.…”

“…13,15 One recent study by Zhang and his colleagues showed that circular RNA circ_0001287 inhibited the proliferation, metastasis, and radiosensitivity of non-small cell lung cancer cells by sponging miR-21. 16 Hao et al reported that circDCUN1D4 suppressed tumor metastasis and glycolysis in lung adenocarcinoma by stabilizing TXNIP expression.…”