Circular RNA and Alzheimer’s Disease

Akhter, Rumana

doi:10.1007/978-981-13-1426-1_19

Cited by 183 publications

(122 citation statements)

References 23 publications

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“…This phenomenon might be attributed to the fact the abundance of protein-coding genes producing various circRNAs and splicing factors and RBPs regulating cir-cRNA formation [18]. CircRNAs are involved in several nervous system diseases such as Alzheimer's disease mediated by ciRS-7 sponging miR-7 [76]. In addition, growing studies highlight the function of circRNAs on glioma progression and tumorigenicity.…”

Section: Circrna and Gliomamentioning

confidence: 99%

Functions and clinical significance of circular RNAs in glioma

et al. 2020

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CircRNAs are a class of single-stranded RNA molecules with a covalently closed loop structure and have been characterized by high stability, abundance, conservation, and display tissue/developmental stage-specific expression, furthermore, based on the abundance in distinct body fluids or exosomes, circRNAs present novel biomarkers and targets for the diagnosis and prognosis of cancers. Recently, the regulatory mechanisms of biogenesis and molecular functions, including miRNAs and RBPs sponge, translation as well as transcriptional and splicing regulation, have been gradually uncovered, although various aspects remained to be elucidated in combination with deep-sequence and bioinformatics. Accumulating studies have indicated that circRNAs are more enriched in neuronal tissues partly due to the abundance of specific genes promoting circularization, suggesting dysregulation of circRNAs is closely related to diseases of the nervous system, including glioma. In this review, we elaborate on the biogenesis, functions, databases as well as novel advances especially involved in the molecular pathways, highlight its great value as diagnostic or therapeutic targets in glioma.

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Section: Circrna and Gliomamentioning

confidence: 99%

Functions and clinical significance of circular RNAs in glioma

et al. 2020

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“…It has been reported that circRNAs are involved in multiple pivotal biological processes, including cell proliferation, cell differentiation, apoptosis, and metabolism, 17-19 and circRNA expression patterns are associated with a variety of diseases, such as malignancies, 21, 22 immune disorders, 23 cardiovascular conditions, 24 and neurological degeneration. 25 To explore the role of circRNAs in IDD, a differential expression profile was produced and was analysed with a bioinformatic approach; the data revealed a promising candidate, CircGLCE. 26 In vivo experiments were performed using a rat tail model, which provides readily detected intervertebral disc degeneration through mechanical injury.…”

Section: Introductionmentioning

confidence: 99%

CircGLCE alleviates intervertebral disc degeneration by regulating apoptosis and matrix degradation through the targeting of miR-587/STAP1

Chen

Zhang

Deng

et al. 2020

Preprint

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BackgroundIntervertebral disc degeneration (IDD) can induce profound global socioeconomic burdens. Recent studies have suggested that circular RNAs might have crucial functions in the progression of IDD. The purpose of this study was to identify a specific circular RNA and to investigate its regulatory mechanism in IDD.MethodsCircGLCE was selected after microarray analyses and was further analysed by RT-qPCR and FISH. After silencing CircGLCE, its function was assessed with RT-qPCR, immunofluorescence analysis and flow cytometry. Based on Sanger sequencing, miR-587 was identified as a direct target of CircGLCE, and it was further examined with RNA pulldown assays, RT-qPCR, dual luciferase assays and FISH. After silencing CircGLCE or miR-587, western blotting, immunofluorescence analysis, and flow cytometry were conducted. STAP1 was assessed by RT-qPCR and luciferase assay, and experiments with silenced and overexpressed miR-587 were performed. A rescue experiment was also included. In an IDD rat model, the in vivo effects of overexpressing CircGLCE on IDD were analysed with imaging techniques, TUNEL staining, FISH, western blotting, H&E staining and immunohistochemistry.ResultsCircGLCE was found to stably exist in the cytoplasm of nucleus pulposus (NP) cells. It was downregulated in IDD. Knockdown of CircGLCE promoted apoptosis and induced the expression of matrix-degrading enzymes in NP cells. CircGLCE served as a miR-587 sponge in NP cells. Inhibiting miR-587 counteracted the IDD-enhancing effect caused by silencing CircGLCE. STAP1 served as the miRNA target that mediated the functions of miR-587. Overexpressing CircGLCE alleviated IDD in vivo.ConclusionsCircGLCE attenuates IDD by regulating the apoptosis of NP cells and by regulating ECM degradation through the targeting of miR-587/STAP1. CircGLCE may be a potential therapeutic target for IDD treatments.

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“…In recent years, with the progression of circRNA research, many researches have reported the participation of circRNAs in the progression of various diseases, including arteriosclerosis (Burd et al, 2010), nervous system disorders (Li et al, 2017b;Akhter, 2018), aging (Westholm et al, 2014), heart failure (Wang et al, 2016), and cancers (Qu et al, 2017;Zhang et al, 2018). Yet, its effect and mechanism in osteogenic differentiation requires more exploration.…”

Section: Discussionmentioning

confidence: 99%

The Circular RNA circRNA124534 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Modulation of the miR-496/β-Catenin Pathway

Pan

Shen

et al. 2020

Front. Cell Dev. Biol.

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Circular RNAs (circRNAs) have been found to be a crucial role in stem cell-associated bone regeneration. However, the functions and underlying mechanisms of circRNAs in the osteogenic differentiation of human dental pulp stem cells (hDPSCs) remain largely unclear. We found that overexpression of circRNA124534 unexpectedly promoted DPSCs osteogenesis in vitro and in vivo. Our results confirmed circRNA124534, acting as a miRNA sponge, directly interacts with miR-496 and consequently regulates β-catenin, which in turn exerts osteogenesis of DPSCs. Enforced expression of miR-496 reversed the osteogenesis of circRNA124534, and suppression of miR-496 enhanced the osteogenic differentiation of DPSCs by promoting β-catenin. In conclusion, our findings demonstrate functions of circRNA124534 in modulating osteogenic differentiation through the miR-496/β-catenin pathway; thus, providing a novel potential target for therapy.

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Circular RNA and Alzheimer’s Disease

Cited by 183 publications

References 23 publications

Functions and clinical significance of circular RNAs in glioma

Functions and clinical significance of circular RNAs in glioma

CircGLCE alleviates intervertebral disc degeneration by regulating apoptosis and matrix degradation through the targeting of miR-587/STAP1

The Circular RNA circRNA124534 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Modulation of the miR-496/β-Catenin Pathway

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