Circular RNA 101368/miR-200a axis modulates the migration of hepatocellular carcinoma through HMGB1/RAGE signaling

Li, Shaling; Gu, Huimin; Huang, Yan; Peng, Qian; Zhou, Rongrong; Yi, Panpan; Chen, Ruochan; Huang, Zebing; Hu, Xingwang; Huang, Yun; Tang, Daolin

doi:10.1080/15384101.2018.1526599

Cited by 57 publications

(43 citation statements)

References 20 publications

(27 reference statements)

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“…Moreover, expression of RAGE itself is also closely associated with the invasiveness and metastatic activities of gastric cancer 6 . Additionally, microRNA is reported to participate in RAGE-ligands mediated migration or cancer progression [46][47][48] . In this study, RAGE was overexpressed to hoist RAGE-mediated signaling and to investigate the effect of RAGE axis on the tumorigenesis of lung cancer.…”

Section: Discussionmentioning

confidence: 99%

RAGE acts as an oncogenic role and promotes the metastasis of human lung cancer

Chen

Chang

et al. 2020

Cell Death Dis

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RAGE (receptor for advanced glycation end-product) is thought to be associated with metastasis and poor prognosis of various types of cancer. However, RAGE is constitutively expressed in the normal lung and down-regulated in cancerous lung, while the opposite evidence shows that RAGE-mediated signaling contributes to the tumorigenesis of lung cancer. Therefore, the role of RAGE in lung cancer progression is still unclear to be further investigated. In this study, RAGE-overexpressed stable clones of human lung cancer A549 cells and two local lung adenocarcinoma cell lines CL1-0 and CL1-5 were utilized to verify the effect of RAGE on lung cancer cells while the in vivo xenograft animal model was further performed to evaluate the role of RAGE in the progression of lung cancer. The growth of A549 cells was inhibited by RAGE overexpression. p53-dependent p21 CIP1 expression contributed to RAGE-induced growth inhibition by suppressing CDK2 kinase activity and retinoblastoma protein (RB) phosphorylation in vitro. On the other hand, RAGE overexpression promoted migration, invasion, and mesenchymal features of lung adenocarcinoma cells through ERK signaling. Furthermore, an in vivo xenograft experiment indicated that RAGE promoted the metastasis of lung cancer cells with p21 CIP1 up-regulation, ERK activation, and the changes of EMT markers. Regarding to the involvement of tumor-associated macrophage (TAM) in the microenvironment, we monitored the expressions of TAM markers including CD68 and CD163 as well as angiogenesis marker CD31 in xenograft slice. The data showed that RAGE might induce the accumulation of TAM in lung cancer cells and further accelerate the in vivo tumor growth. In summary, our study provides evidence indicating the distinct in vitro and in vivo effects of RAGE and related mechanisms on tumor growth and metastasis, which shed light on the oncogenic role of RAGE in lung cancer.

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Section: Discussionmentioning

confidence: 99%

RAGE acts as an oncogenic role and promotes the metastasis of human lung cancer

Chen

Chang

et al. 2020

Cell Death Dis

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“…Moreover, miR-200a-3p and miR-141-3p are downregulated in the plasma samples of patients with endometriosis compared with that in healthy controls, suggesting that these miRNAs could be used as putative noninvasive diagnostic biomarkers of endometriosis 38 . Li et al 39 found that inhibition of miR-200a could promote migration and high mobility group box 1 expression, while decreasing E-cadherin expression in HCC cell lines. Another study showed that EMT progression was inhibited in miR-200a-3p -overexpressing papillary thyroid cancer (PTC) cells 40 .…”

Section: Discussionmentioning

confidence: 99%

CircATRNL1 promotes epithelial–mesenchymal transition in endometriosis by upregulating Yes-associated protein 1 in vitro

et al. 2020

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Endometriosis is a common and benign gynecological disorder but exhibits malignant features. However, the underlying pathogenesis and pathophysiology of endometriosis remain unclear. Circular RNAs have been demonstrated to participate in the occurrence and progression of multiple diseases. This study was aimed to explore the roles of circATRNL1 in endometriosis in vitro. Based on the results of reverse transcription-quantitative polymerase chain reaction analysis, we found significant upregulation of circATRNL1 and Yes-associated protein 1 (YAP1), while downregulation of miR-141-3p and miR-200a-3p in ectopic tissues compared to eutopic tissues. The immunohistochemistry and western blot analysis showed differentially expressed epithelial–mesenchymal transition (EMT) markers between EuEM and EcEM tissues. The in vitro assays indicated that overexpression of circATRNL1 could promote the proliferation, migration, and invasion of Ishikawa cells, and induce EMT process, while circATRNL1 silencing showed the opposite effect. The mechanical investigation indicated that circATRNL1 upregulated YAP1 by sponging miR-141-3p and miR-200a-3p. Gain-of-function assays validated the inhibitory function of miR-141-3p and miR-200a-3p in endometriosis. The results of rescue assays confirmed the function of circATRNL1–miR-141-3p/miR-200a-3p–YAP1 axis on Ishikawa cells. Our findings demonstrate that abnormal upregulation of circATRNL1 regulates cell proliferation and motility and promotes EMT process via the miR-141-3p/miR-200a-3p–YAP1 axis in vitro, which could contribute to the progression of endometriosis.

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“…However, currently there are no effective biomarkers for the early diagnosis of HCC, and the molecular mechanisms underlying HCC metastasis remains unclear. 35,36 EPDR1, a member of piscine brain glycoproteins, plays a role in cell adhesion. To gain detailed insights into the potential functions of EPDR1 and its regulatory network in HCC, we performed bioinformatics analysis using publicly available data, which we hope will benefit future research related to HCC.…”

Section: Discussionmentioning

confidence: 99%

EPDR1 correlates with immune cell infiltration in hepatocellular carcinoma and can be used as a prognostic biomarker

Chen

Zhang

2020

J Cellular Molecular Medi

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Primary liver cancer (PLC) is one of the malignant tumors of the digestive system, which poses a huge threat towards human health; hepatocellular carcinoma (HCC) is the major histological type of PLC. 1 Globally, HCC ranks sixth in the number of incidences and second in the mortality rate among all malignancies. 2,3 Moreover, the number of HCC incidences is rising, and it is expected that the annual incidences will be beyond 1 million by 2025. 4,5 Currently, the diagnosis of HCC is mainly based on the detection of serum alpha-fetoprotein (AFP), B-ultrasound, and computed tomography imaging. However, the misdiagnosis and missed diagnosis rates are very high. 6-8 Owing to the insidious onset, poor tumor biological behavior, and rapid progress of HCC, most patients reach advanced stage of the disease and miss the opportunity for radical treatment. Despite the advances in research related to HCC biomarkers, in recent years, lack of specific markers for tumor sub-types, disease stages, or prognosis remains an important gap in the understanding and treatment

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Circular RNA 101368/miR-200a axis modulates the migration of hepatocellular carcinoma through HMGB1/RAGE signaling

Cited by 57 publications

References 20 publications

RAGE acts as an oncogenic role and promotes the metastasis of human lung cancer

RAGE acts as an oncogenic role and promotes the metastasis of human lung cancer

CircATRNL1 promotes epithelial–mesenchymal transition in endometriosis by upregulating Yes-associated protein 1 in vitro

EPDR1 correlates with immune cell infiltration in hepatocellular carcinoma and can be used as a prognostic biomarker

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