Circular RNA-100290 promotes cell proliferation and inhibits apoptosis in acute myeloid leukemia cells via sponging miR-203

Fan, Hongyu; Li, Yuying; Liu, Chunshui; Liu, Yingmin; Bai, Jing; Li, Wei

doi:10.1016/j.bbrc.2018.11.002

Cited by 52 publications

(42 citation statements)

References 20 publications

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“…And circular RNA-100290 regulates cell proliferation and apoptosis via sponging miR-203/Rab10 axis. 34 Analogously, we found that Rab10 expression was elevated in AML bone marrow and cells. Moreover, Rab10 expression was negatively regulated by miR-193a-5p but positively regulated by TUG1.…”

Section: Dovepresssupporting

confidence: 56%

<p>LncRNA TUG1 Regulates Cell Viability and Death by Regulating miR-193a-5p/Rab10 Axis in Acute Myeloid Leukemia</p>

Li¹,

Wang²

2020

OTT

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Background: Acute myeloid leukemia (AML) is a serious threat to human health. Long non-coding RNA (lncRNA) Taurine-Upregulated Gene1 (TUG1) has been reported to participate in the development and progression of several cancers, including AML. Herein, we aimed to investigate the pathognomonic role of TUG1 in AML cells and its potential mechanistic pathway. Methods: Quantitative real-time PCR (qRT-PCR) assay was applied to detect the expression levels of lncRNA TUG1, miR-193a-5p and Rab10 in AML bone marrow and cell lines. The CCK-8 assay was conducted to assess the cell viability of AML HL-60 and NB4 cells and cell apoptotic assay was performed to assess the cell death. Dual-luciferase reporter assay was carried out to clarify the relationships among TUG1, miR-193a-5p and Rab10. Also, the protein level of Rab10 was examined by Western blot assay. Results: LncRNA TUG1 was up-regulated in AML bone marrow and cells. Functional analysis showed that the silencing of TUG1 suppressed cell viability, while promoted cell death in AML HL-60 and NB4 cells. TUG1 targeted miR-193a-5p and negatively regulated miR-193a-5p expression. Overexpressed miR-193a-5p resulted in the decrease of cell viability and the increase in the cell death in AML cells. Restoration experiments proved that TUG1 regulated the cell viability and death of AML cells through regulating the miR-193a-5p/Rab10 axis. Rab10 was a direct target of miR-193a-5p and was inversely regulated by miR-193a-5p. TUG1 regulated the cell viability and death of AML cells through upregulating Rab10. Conclusion: Silencing of lncRNA TUG1 induces a cytotoxic effect on AML cell lines through sponging miR-193a-5p and the suppression of Rab10.

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Section: Dovepresssupporting

confidence: 56%

<p>LncRNA TUG1 Regulates Cell Viability and Death by Regulating miR-193a-5p/Rab10 Axis in Acute Myeloid Leukemia</p>

Li¹,

Wang²

2020

OTT

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“…An oncogenic activity of circRNA was also shown by DLEU2, which was highly expressed in AML, that inhibited miR-496 expression to promote cell proliferation and inhibit cell apoptosis [157]. A circular RNA 100290, which as an oncogenic circRNA was upregulated in AML, showed that it sponged miR-203 to control AML cell proliferation and apoptosis [152]. Moreover, Shang et al demonstrated the circRNA PAN3 controlled AML chemoresistance by sequestering miR-153-5p and miR-183-5p, [153].…”

Section: Prognostic Value Of Lncrnas In Acute Myeloid Leukemiamentioning

confidence: 97%

Role of microRNAs, circRNAs and long noncoding RNAs in acute myeloid leukemia

et al. 2019

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Acute myeloid leukemia (AML) is a malignant tumor of the immature myeloid hematopoietic cells in the bone marrow (BM). It is a highly heterogeneous disease, with rising morbidity and mortality in older patients. Although researches over the past decades have improved our understanding of AML, its pathogenesis has not yet been fully elucidated. Long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs) are three noncoding RNA (ncRNA) molecules that regulate DNA transcription and translation. With the development of RNA-Seq technology, more and more ncRNAs that are closely related to AML leukemogenesis have been discovered. Numerous studies have found that these ncRNAs play an important role in leukemia cell proliferation, differentiation, and apoptosis. Some may potentially be used as prognostic biomarkers. In this systematic review, we briefly described the characteristics and molecular functions of three groups of ncRNAs, including lncRNAs, miRNAs, and circRNAs, and discussed their relationships with AML in detail.

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“…Their abnormal tissue-and time-specific expression [10] may play crucial roles in disease development and progression. Several recent studies revealed a close association of particular circRNAs with hematopoietic malignancies, especially AML [78][79][80][81][82][83][84][85][86]. As AML is the malignancy most related to MDS, we summarize the roles of particular circRNAs in this disease in more detail.…”

Section: Circrnas In Hematopoiesismentioning

confidence: 97%

“…Another circRNA related to AML cells is hsa_circ_100290 (circularized transcript of the SLC30A7 gene). This circRNA is downregulated not only in AML [81] but also in other cancers, suppressing the proliferation, invasion, and migration of cancer cells [82]. Fan et al [81] found that hsa_circ_100290 expression is increased in AML and that its silencing induces cell cycle arrest and apoptosis.…”

Section: Circrnas In Amlmentioning

confidence: 99%

Circular RNAs in Hematopoiesis with a Focus on Acute Myeloid Leukemia and Myelodysplastic Syndrome

Merkerová

Krejčík

Szikszai

et al. 2020

IJMS

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Circular RNAs (circRNAs) constitute a recently recognized group of noncoding transcripts that function as posttranscriptional regulators of gene expression at a new level. Recent developments in experimental methods together with rapidly evolving bioinformatics approaches have accelerated the exploration of circRNAs. The differentiation of hematopoietic stem cells into a broad spectrum of specialized blood lineages is a tightly regulated process that depends on a multitude of factors, including circRNAs. However, despite the growing number of circRNAs described to date, the roles of the majority of them in hematopoiesis remain unknown. Given their stability and disease-specific expression, circRNAs have been acknowledged as novel promising biomarkers and therapeutic targets. In this paper, the biogenesis, characteristics, and roles of circRNAs are reviewed with an emphasis on their currently recognized or presumed involvement in hematopoiesis, especially in acute myeloid leukemia and myelodysplastic syndrome.

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Circular RNA-100290 promotes cell proliferation and inhibits apoptosis in acute myeloid leukemia cells via sponging miR-203

Cited by 52 publications

References 20 publications

<p>LncRNA TUG1 Regulates Cell Viability and Death by Regulating miR-193a-5p/Rab10 Axis in Acute Myeloid Leukemia</p>

<p>LncRNA TUG1 Regulates Cell Viability and Death by Regulating miR-193a-5p/Rab10 Axis in Acute Myeloid Leukemia</p>

Role of microRNAs, circRNAs and long noncoding RNAs in acute myeloid leukemia

Circular RNAs in Hematopoiesis with a Focus on Acute Myeloid Leukemia and Myelodysplastic Syndrome

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