Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A

Abstract

Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude mice to observe tumor growth in vivo . The interaction circ_0000654, miR-149-5p, and INHBA was validated. circ_0000654 and INHBA were up-regulated but miR-149-5p was down-regulated in GC. circ_0000654 absorbed miR-149-5p to target INHBA. Silencing circ_0000654inhibited the progress of GC cell biology. Oppositely, restoring circ_0000654 enhanced the growth of GC cells. Inhibiting miR-149-5p rescued down-regulated circ_0000654-induced anti-tumor effect on GC. circ_0000654 silence or miR-149-5p overexpression limited the growth of GC tumors in vivo . Obviously, circ_0000654 facilitates the growth of GC cells through absorbing miR-149-5p to up-regulate INHBA.

circHIPK3 regulates cell proliferation and migration by sponging microRNA-124 and regulating serine/threonine kinase 3 expression in esophageal squamous cell carcinoma

“…Circ_0005320 facilitates oral squamous cell carcinoma tumorigenesis via sponging miRNA-486-3p and miRNA-637 [ 9 ]. Circ_0000654 that absorbs miRNA-149-5p to upregulate inhibin-beta A enhances the growth of gastric cancer cells [ 10 ]. Moreover, Zheng Q et al [ 11 ] has discovered that circHIPK3 is the most abundant among the three distinct types of splicing bodies in the circRNAs of the HIPK3 gene, which plays a critical role in cells growth through sponging multiple miRNAs.…”

Correlation of INHBA Overexpression with Pathological Features, Antitumor Immune Response and Clinical Prognosis in Cervical Cancer

“…Several studies found that abnormal expression of INHBA exerted various biological functions in different tumor development processes. For instance, the inhibitory role of INHBA in the growth of lung cancer and head and neck squamous cell carcinoma is not obvious, but it enhances the invasive ability of tumor cells, which involves many processes of the body and embryonic development, including cell growth, differentiation, apoptosis, homeostasis and so on [ 4 ]. The expression level of INHBA in various malignant tumor tissues, such as gastric cancer and breast cancer, is significantly increased.…”