Circular Nonuniform Electric Field Gel Electrophoresis for the Separation and Concentration of Nanoparticles

Liu, Lu; Yang, Ruilin; Cui, Jiaxuan; Chen, Peng; Ri, Hyok Chol; Sun, Huaze; Piao, Xiangfan; Li, Minshu; Pu, Qiaosheng; Quinto, Maurizio; Zhou, John L.; Shang, Han Lin; Li, Donghao

doi:10.1021/acs.analchem.2c01313

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…Precise manipulation of fluids/particles has been considerably advanced and accompanied by but not limited to microfluidics development for clinical diagnosis and self-assembly of sensors. − Up to this date, several manipulation techniques have been developed based on various mechanisms, such as acoustics, , magnetism, , electric fields, , and optofluidics. , Although they are promising, acoustic and electrowetting methods require complex and costly chip fabrication. The application of magnetism methods is limited by magnetic particles.…”

Section: Introductionmentioning

confidence: 99%

Light-Controlled Particle Enrichment Patterns in Droplets

Chen

et al. 2023

Anal. Chem.

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Precision manipulation of particle-enrichment patterns in droplets is challenging but important in biochemical analysis and clinical diagnosis. Herein, a light strategy for precisely manipulating particle enrichment patterns is reported. Focused laser irradiation to the droplet induces a Marangoni flow owing to a localized photothermal effect, which carries in-droplet particles and concentrates them at the laser-spot-acted region. Owing to high flexibility of light, multiple particle-enriched sites are formed in a droplet, and the concentrated particles can be transported and reconstructed on demand. In addition to the island-like enrichment pattern, this optical particle manipulation strategy enables the formation of various particle-enriched patterns, such as the line-shape and circle-shape patterns. Further, light directly acts on the working fluid instead of target particles, considerably weakening dependence on particle properties. For particles whose density is similar to that of the working fluid, a portion of particles can still be concentrated. It is also found that only a small portion of submicron particles can be concentrated, while nanoparticles are hardly concentrated by this light strategy. Moreover, high reconfigurability of light enables in-parallel high-throughput operations, which is demonstrated using two laser beams to form two particle enrichment sites in a droplet simultaneously. Finally, this light strategy is also demonstrated by concentrating cells and nucleic acid molecules. This work paves the way for the applications of optofluidics in cell sorting, point-of-care analysis, and drug screening.

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Section: Introductionmentioning

confidence: 99%

Light-Controlled Particle Enrichment Patterns in Droplets

Chen

et al. 2023

Anal. Chem.

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“…In the field of microfluidics, ionic surfactants are used to control the attachment and peeling of substrates, thereby inhibiting the interactions between liquids and substrates [5]. The separation and enrichment of micro-and nano-scale materials can be achieved by using the relationship between the charge characteristics and electrophoretic behavior of microscale and nano-scale substances combined with the target distribution of the electric field of a circular electric field and the regulation of the distribution of an electric field on microscale and nano-scale substances [6]. In these fields, obtaining a fast and accurate solution of the local electric field of particles and an understanding of the interactions between particles and materials and the behavior of the electric fields of particles are vital to the study of such problems.…”

Section: Introductionmentioning

confidence: 99%

A Novel Mutual-Coupling Dipole Model Considering the Interactions between Particles

Kang

Zhang²,

Shi³

et al. 2022

Coatings

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The interactions between two or more particles and the calculation of the local electric field are widely applied in many fields, such as those of insulation, biology, medicine, and microfluidics. The dipole approximation model, which is a classical electric field calculation method, has been widely used in many fields to solve for the local electric field in a multi-particle system, but it does not consider the interactions between particles; as a result, it is easily limited by the calculation situation, and it generates a large calculation error when the distance between particles is small. Based on the physical essence of an interaction between two particles, a concept of the mutual-coupling dipole moment caused by the interactions between particles is defined for the first time. Moreover, by combining the calculation process of the dipole moment and the electric field of polarization, a novel mutual-coupling dipole model considering the interactions between particles is proposed in this paper, and analytical expressions of the local electric field that consider the interaction between two particles are obtained, thus compensating for the large error in the electric field calculation caused by the dipole approximation model when the distance between particles is small. In this paper, a mutual-coupling dipole model considering particle interactions is proposed. This model can effectively reflect the interactions between particles when the distance between particles D/R is less than 0.6 and accurately calculate the local electric fields of the particles. These results can be effectively used to investigate the interactions between particles and the control of particles in electric fields in many fields, such as in the calculation of the insulation of mixed dielectrics, the microscopic transport of medicines, the control of bio-cells and micro-fluids in electric fields, and environmental governance.

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“…This method can reinforce the throughput of ICEO vortices and enables it to integrate with simple and economical sensors to accomplish disease diagnosis and environmental monitoring. Manipulation of micro/nanoscale objects plays an essential role in many detection and sensing applications, such as rapid diagnostics of marine pests, 1 disease diagnosis, 2 and water quality inspection. 3 For these, many techniques based on different physical principals have been primarily developed, which can be either passive or active methods based on features of driving fields.…”

mentioning

confidence: 99%

“…Manipulation of micro/nanoscale objects plays an essential role in many detection and sensing applications, such as rapid diagnostics of marine pests, disease diagnosis, and water quality inspection . For these, many techniques based on different physical principals have been primarily developed, which can be either passive or active methods based on features of driving fields .…”

mentioning

confidence: 99%

Enrichment and Selection of Particles through Parallel Induced-Charge Electro-osmotic Streaming for Detection of Low-Abundance Nanoparticles and Targeted Microalgae

Chen

Liu

et al. 2023

Anal. Chem.

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Manipulation of micro- and nanoscale objects is an essential procedure in many detection and sensing applications, including disease diagnosis and environmental monitoring. Induced-charge electro-osmotic (ICEO) vortices present excellent advantages in the enrichment and selection of micro/nanoscale particles for downstream detection due to gentle conditions and contactless operation, but the application of this method is currently constrained by the throughput. Double-layer charging at the ends of bipolar electrodes can maintain a continuous flow of electric current in the fluidically isolated channels, which provides a feasible method to manipulate particles using parallel ICEO vortices, promoting throughput of particle manipulation without compromising efficiency and overcoming the complicated ohmic contact of electrodes. Encouraged by these, we put forward a novel method with parallel ICEO vortices to manipulate micro/nanoscale samples for downstream detection. First, we study the extension regulation of the low-frequency electric field and mediating effect of the open BPEs on the extended electric field and characterize electric equilibrium states of microparticles and their voltage dependence. Afterward, we leverage this method to enrich nanoparticles for detection of low-abundance nanoparticles with about 20- and 40-fold fluorescence intensities by integrating with a simple fiber-optic sensor. Furthermore, this technique is engineered for the selection of targeted microalgae to continuously detect their proliferation behaviors by combining with a homemade electrical impedance spectroscopy device. This method can reinforce the throughput of ICEO vortices and enables it to integrate with simple and economical sensors to accomplish disease diagnosis and environmental monitoring.

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Circular Nonuniform Electric Field Gel Electrophoresis for the Separation and Concentration of Nanoparticles

Cited by 7 publications

References 45 publications

Light-Controlled Particle Enrichment Patterns in Droplets

Light-Controlled Particle Enrichment Patterns in Droplets

A Novel Mutual-Coupling Dipole Model Considering the Interactions between Particles

Enrichment and Selection of Particles through Parallel Induced-Charge Electro-osmotic Streaming for Detection of Low-Abundance Nanoparticles and Targeted Microalgae

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