Circular mitochondrial DNA

Bruggen, E.F.J. Van; Borst, Piet; Ruttenberg, G.J.C.M.; Gruber, M.; Kroon, A.M.

doi:10.1016/0005-2787(66)90210-3

Cited by 109 publications

(25 citation statements)

References 3 publications

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“…(Robinow and Kellenberger, 1994)). Similar DNA-containing structures were later discovered in mitochondria and plastids (Iwamura, 1960;Nass, 1966;Ris and Plaut, 1962;van Bruggen et al, 1966). This agrees with evolutionary studies, which suggest that both these organelles have prokaryotic ancestry (rev.…”

Section: Nucleoidssupporting

confidence: 88%

The influence of ATP-dependent proteases on a variety of nucleoid-associated processes

Ambro

Pevala

Bauer

et al. 2012

Journal of Structural Biology

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Section: Nucleoidssupporting

confidence: 88%

The influence of ATP-dependent proteases on a variety of nucleoid-associated processes

Ambro

Pevala

Bauer

et al. 2012

Journal of Structural Biology

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“…This indicates that this DNA is double stranded. Recent electron micrographs of extracted DNA (12,21,27) have shown that mitochondrial DNA's from several vertebrate sources have a circular configuration whereas nuclear DNA's from the same cells are linear. The circles have a length of about 5 /u which is equivalent to a molecular weight of about 10 X 106.…”

Section: Discussionmentioning

confidence: 99%

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

Parsons¹,

Rustad²

1968

The Journal of Cell Biology

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A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial labeling. Low radioautograph background suggests that mitochondrial DNA is synthesized at the mitochondria themselves. All mitochondria incorporated thymidine-3H within one population-doubling time. Grain counts also showed that the amount of mitochondrial label was retained for four generations and that this label remained randomly distributed among all mitochondria during this time. The results are not consistent with any theory of de-novo or "microbody" origin of mitochondria, but do support the hypothesis that mitochondria are produced by the growth and division of preexisting mitochondria. The stability of the mitochondrial DNA and its distribution among daughter mitochondria satisfy two prerequisites for a genetic material. The possibility is discussed that some of the genetic information for the mitochondrion is contained in the DNA associated with this organelle.

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“…Back in the Netherlands in 1965, I decided to combine my old experience with mitochondria with my new knowledge of nucleic acids and set up a project on nucleic acids in mitochondria. With Van Bruggen and Ruttenberg I soon found that animal mitochondrial DNAs consist of 5-micron circles [8,9], and with quantitative DNA renaturation experiments we showed unambiguously that there was only a single type of circle in the mitochondrial DNA. This proved that the genetic content of mitochondrial DNA is very limited and completely insufficient to encode the proteins known to reside in mitochondria [8].…”

mentioning

confidence: 80%