Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Ai, Nini; Yu, Zonggang; Xu, Xueli; Liufu, Sui; Wang, Kaiming; Huang, Shaoxiong; Li, Xintong; Liu, Xiaolin; Chen, B; Ma, Haiming; Yin, Yulong

doi:10.3390/ijms24129859

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…The expression level of mRNA was detected with high or significant differences, but different results were found when detecting the corresponding protein abundance. Resulting in the expression of mRNA measured by experiment is not consistent with the real protein situation (Ai et al, 2023;Wang et al, 2023;Yu et al, 2023). Existing literature also underscores the influence of age on gene expression levels in mammalian tissues (Ahn et al, 2019;Bloom et al, 2020).…”

Section: Introductionmentioning

confidence: 85%

Integrated analysis of muscle transcriptome, miRNA, and proteome of Chinese indigenous breed Ningxiang pig in three developmental stages

Chen,

Xiao,

Yang

et al. 2024

Front. Genet.

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The Ningxiang pig, a distinguished local breed in China, is recognized for its good meat quality traits. This study examines the proteomics of Ningxiang pigs at three developmental stages and delves into the upstream transcriptomics of these proteomics. Such an analysis facilitates a deeper understanding of the molecular interplay between proteins and transcriptomes in the Ningxiang pig muscle, influencing muscle growth and development. In this research, we analyzed the muscles of Ningxiang pigs at three developmental stages: 30 days in weaned piglets, 90 days in nursery pigs, and 210 days in late fattening pigs. There a total of 16 differentially co-expressed miRNAs (ssc-miRNA-1, ssc-miRNA-378, ssc-miRNA-143, ssc-miRNA-30e, etc.), 74 differentially co-expressed mRNA (PLIN3, CPT2, IGF2 and HSP90AB1, etc.) have been identified in the three stages. 572 differentially abundant proteins (DAPs) (APOC3, NDUFA2, HSPD1, ATP5E, PDHA1, etc.) were readily identified by comparing different time periods. According to the KEGG enrich pathway results that DAPs most enriched in growth and development pathways, immune mechanism pathways and maintaining functions of physical. Through short time-series expression miner (STEM) association analysis, a total of 571 negative miRNA-mRNA interaction pairs and 2 negative miRNA-mRNA-protein (Chr05_11955-Pig.17268.1-ATP5F1B, ssc-miR-194a-3p-Pig.15802.1-ACY1) interaction pairs were found. Our study provides a theoretical basis on molecular mechanism for the study of IMF deposition, muscle growth and immunity in Ningxiang pig breed.

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Section: Introductionmentioning

confidence: 85%

Integrated analysis of muscle transcriptome, miRNA, and proteome of Chinese indigenous breed Ningxiang pig in three developmental stages

Chen,

Xiao,

Yang

et al. 2024

Front. Genet.

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“…Notably, this group also uncovered that circ-SIRT1 contributed to stabilizing SIRT1 protein post-translationally via recruiting USP22 and further accelerating deubiquitination on SIRT1 protein [39]. Additionally, circTTN, a circular intronic RNA, was confirmed to repress the transcription and myogenesis of TTN through forming heterotypic complexes via recruiting PURB proteins [40]. An antisense circRNA, named circSCRIB, was illustrated to retard the splicing and translation of its parental gene SCRIB, enhancing BC progression [41].…”

Section: Affecting Parental Gene Expressionmentioning

confidence: 96%

Circular RNAs in Breast Cancer: An Update

Bao,

Li,

Zhao

et al. 2024

Biomolecules

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Breast cancer (BC), characterized by high heterogeneity, is the most commonly reported malignancy among females across the globe. Every year, many BC patients die owing to delayed diagnosis and treatment. Increasing researches have indicated that aberrantly expressed circular RNAs (circRNAs) are implicated in the tumorigenesis and progression of various tumors, including BC. Hence, this article provides a summary of the biogenesis and functions of circRNAs, as well as an examination of how circRNAs regulate the progression of BC. Moreover, circRNAs have aroused incremental attention as potential diagnostic and prognostic biomarkers for BC. Exosomes enriched with circRNAs can be secreted into the tumor microenvironment to mediate intercellular communication, affecting the progression of BC. Detecting the expression levels of exosomal circRNAs may provide reference for BC diagnosis and prognosis prediction. Illuminating insights into the earlier diagnosis and better treatment regimens of BC will be potentially available following elucidation of deeper regulatory mechanisms of circRNAs in this malignancy.

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“…Ai et al found differentially expressed circRNA (circTTN) in Ningxiang pig muscle tissue, and further veri ed its role in muscle development on cells. The results showed that circTTN inhibited host gene transcription and myogenesis by binding PURB protein [22]. However, these results still remain largely unclear for dissecting the mechanism of skeletal muscle development.…”

Section: Introductionmentioning

confidence: 98%

Transcriptome sequencing reveals candidate genes and signaling pathways for skeletal muscle development in Ningxiang pig

Yu,

Xu,

et al. 2023

Preprint

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Background Ningxiang pig is resistant to roughage, delicious in meat quality and rich in polyunsaturated fatty acids. However, its growth rate is slow and its meat yield is low, so it is urgent to analyze the molecular mechanism of its muscle growth and development. Genes are important molecules involved in muscle growth and development. In this study, transcripts of difference tables of Ningxiang pig at two developmental stages (30 days and 150 days after birth) were identified by transcriptome sequencing, and the molecular mechanism of their regulation of muscle development was further explored. Result A total of 22,411 transcripts were identified by transcriptome sequencing, and 9,021 transcripts were obtained after screening with TPM ≥ 1. Differential expression analysis obtained 1,322 differentially expressed transcripts, 978 of which were up-regulated and 344 down-regulated. There were 1,125 known and 197 novel identified differentially expressed transcripts, 823 up-regulated and 302 down-regulated in known transcripts, and 155 up-regulated and 42 down-regulated in novel identified transcripts. RT-qPCR verification is consistent with sequencing results. After GO enrichment analysis of differentially expressed genes, 15 known genes (15 transcripts) and 6 new genes (8 transcripts) related to muscle were screened. KEGG enrichment found that muscle-related genes were significantly enriched in FoxO, MAPK, cAMP, IL-17 and PI3K-Akt signal pathways. The prediction of PPI and transcription factors of 15 known genes showed that there were significant interactions among 9 proteins (ATF3-EGR1-EGR2-FOS-HOMER1-KLF5, KLHL40-LMOD2-MYOZ2), and there were 8 transcription factors (KLF5, COPS2, FOXN2, EGR2, ATF3, EGR1, FOS, PAX7) among the 15 proteins. To sum up, 15 known genes and 6 novel identified genes related to muscle development in Ningxiang pigs were identified by transcriptome sequencing. These genes regulate muscle development via FoxO, MAPK, cAMP, IL-17 and PI3K-Akt signaling pathways, and there is a significant interaction between them, and they participate in gene expression regulation as transcription factors. Conclusion To sum up, 15 known genes and 6 novel identified genes related to muscle development in Ningxiang pigs were identified by transcriptome sequencing. These genes mainly regulate muscle development through FoxO, MAPK, cAMP, IL-17 and PI3K-Akt signaling pathways, and there is a significant interaction between them, and they participate in gene expression regulation as transcription factors. These results provide theoretical basis for the study of the mechanism of muscle development in Ningxiang pig.

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Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Cited by 5 publications

References 43 publications

Integrated analysis of muscle transcriptome, miRNA, and proteome of Chinese indigenous breed Ningxiang pig in three developmental stages

Integrated analysis of muscle transcriptome, miRNA, and proteome of Chinese indigenous breed Ningxiang pig in three developmental stages

Circular RNAs in Breast Cancer: An Update

Transcriptome sequencing reveals candidate genes and signaling pathways for skeletal muscle development in Ningxiang pig

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