Circular dichroism analysis of the calicheamicin-DNA interaction revisited

Proni, Gloria; Tami, Kristi; Berova, Nina; Ellestad, George A.

doi:10.1016/j.jpba.2017.05.005

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…CD spectra of DNA extracted from rat hepatocyte alone (100 μg/ml) and in the presence of some concentrations of OEO/thymol were recorded with CD spectrophotometer (CD 215, Aviv, USA) equipped with a quartz cuvette with a path length of 1 cm. All spectra were studied in far-UV range 94 , 95 .…”

Section: Methodsmentioning

confidence: 99%

In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA

Jamali

Kavoosi

Safavi

et al. 2018

Sci Rep

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Oliveria decumbens is an Iranian endemic plant used extensively in traditional medicine. Recently, some studies have been performed on biological effects of Oliveria essential oil (OEO). However, to our knowledge, the anticancer activity of OEO has not been reported. Based on our GC/MS analysis, the basic ingredients of OEO are thymol, carvacrol, p-cymene and γ-terpinene. Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. Furthermore, immunoblotting studies revealed the activation of intrinsic and maybe extrinsic apoptosis pathways by OEO/thymol. Additionally, in-vitro experiments, indicated that OEO/thymol interacts with DNA via minor grooves confirmed by docking method. Altogether, our reports underlined the potential of OEO to be considered as a new candidate for cancer therapy.

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Section: Methodsmentioning

confidence: 99%

In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA

Jamali

Kavoosi

Safavi

et al. 2018

Sci Rep

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“…The detection of DNA interactions with low-molecular compounds, including the drugs mentioned above, is mainly based on the optic methods of analysis (e.g., UV-vis [11] and fluorescence [12] spectroscopy, circular dichroism [13], and surface plasmon resonance [14]). They can be combined with chromatography or electrophoresis for separation and combined with DNA for the concentration of the analyte molecules and their adducts.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

Porfireva

Vorobev

Babkina

et al. 2019

Sensors

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A new voltammetric DNA sensor has been developed for doxorubicin determination on the platform of a glassy carbon electrode (GCE) covered with electropolymerized Azure B film and physically adsorbed native DNA. The redox properties of polymeric Azure B were monitored at various pH and scan rates. DNA application decreased the peak currents related to polymeric and monomeric forms of the dye, whereas incubation in doxorubicin solution partially restored the peaks in accordance with the drug and DNA concentration. The relative shift of the cathodic peak current caused by doxorubicin depended on the nominal DNA concentration and its application mode. In optimal conditions, the DNA sensor makes it possible to determine between 0.1 μM to 0.1 nM doxorubicin (limit of detection 7 × 10−11 M). The DNA sensor was tested on commercial doxorubicin formulations and on artificial samples the mimicked electrolyte content of human serum.

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