Circuit analysis reveals a neural pathway for light avoidance in Drosophila larvae

Sorkaç, Altar; Savva, Yiannis A.; Savaş, Doruk; Talay, Mustafa; Barnea, Gilad

doi:10.1038/s41467-022-33059-5

Cited by 4 publications

(3 citation statements)

References 37 publications

(48 reference statements)

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“…As shown in Figure S3A, PTTH immunosignal showed a rhythm in the cell bodies; however we found that this rhythm was not dependent on the clock. (This cycling of PTTH staining in the cell bodies may be dependent on photic inputs, similar to the role of PTTH in light avoidance during larval stages (Yamanaka et al, 2013;Sorkac et al, 2022).) By contrast, PTTH immunoreactivity of the axonal terminals onto the PG showed clock-dependent daily rhythmicity.…”

Section: Coupling Between Central and Peripheral Circadian Clocks 10mentioning

confidence: 55%

“…To determine which LNv neurons signal to PTTHn and whether this contact includes synaptic transmission, we performed a connectomic analysis by GFP reconstitution across synapses (syb-GRASP) (Macpherson et al, 2015), BAcTrace (Cachero et al, 2020), and trans-Tango MkII (Sorkac et al, 2022), in adult pharate animals. This work was necessary because the PTTHn can no longer be detected starting shortly after eclosion (Liu et al, 2016) and are therefore not included in the available EM-based connectomic datasets that are based on older adult flies (Scheffer et al, 2020).…”

Section: All Pdf-positive Slnv Signal Non-synaptically To the Ptthnmentioning

confidence: 99%

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Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock inDrosophila

Cavieres-Lepe

Amini

Nässel

et al. 2023

Preprint

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Circadian clocks impose daily periodicities to behavior, physiology, and metabolism. This control is mediated by a central clock and by peripheral clocks, which are synchronized to provide the organism with a unified time through mechanisms that are not fully understood. Here, we characterized in Drosophila the cellular and molecular mechanisms involved in coupling the central clock and the peripheral clock located in the prothoracic gland (PG), which together control the circadian rhythm of emergence of adult flies. The time signal from central clock neurons is transmitted via small neuropeptide F (sNPF) to neurons that produce the neuropeptide Prothoracicotropic Hormone (PTTH), which is then translated into circadian oscillations of Ca2+ concentration and daily changes in PTTH levels. Rhythmic PTTH signaling is required at the end of metamorphosis, and transmits time information to the PG by imposing a daily rhythm to the expression of the PTTH receptor tyrosine kinase (RTK), TORSO, and of ERK phosphorylation, a key component of PTTH transduction. In addition to PTTH, we demonstrate that signaling mediated by other RTKs contribute to the rhythmicity of emergence. Interestingly, the ligand to one of these receptors (Pvf2), plays an autocrine role in the PG, which may explain why both central brain and PG clocks are required for the circadian gating of emergence. Our findings show that the coupling between the central and the PG clock is unexpectedly complex and involves several RTKs that act in concert, and could serve as a paradigm to understand how circadian clocks are coordinated.

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Section: Coupling Between Central and Peripheral Circadian Clocks 10mentioning

confidence: 55%

Section: All Pdf-positive Slnv Signal Non-synaptically To the Ptthnmentioning

confidence: 99%

Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock inDrosophila

Cavieres-Lepe

Amini

Nässel

et al. 2023

Preprint

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show abstract

“… 111 In neonatal mice, photoaversion has been further mapped to a specific M1 class of ipRGCs, 112 and a specific photoreceptor pathway for photophobia in Drosophila larvae has been identified, which, due to their projection regions controlling circadian photoentrainment, is likely functionally related to ipRGCs. 113 ipRGCs persist during normal aging but are susceptible to degeneration even in mature adults in AD, PD, glaucoma, and diabetic retinopathy. 114 Our data indicate that ipRGCs are functionally maintained in light aversion in mice up to 12 months old, regardless of whether they are localized to the GCL or are displaced in the INL.…”

Section: Discussionmentioning

confidence: 99%

Preservation of Intrinsically Photosensitive Retinal Ganglion Cells (ipRGCs) in Late Adult Mice: Implications as a Potential Biomarker for Early Onset Ocular Degenerative Diseases

Matynia,

Recio,

Myers

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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Purpose Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in non–image-forming visual functions. Given their significant loss observed in various ocular degenerative diseases at early stages, this study aimed to assess changes in both the morphology and associated behavioral functions of ipRGCs in mice between 6 (mature) and 12 (late adult) months old. The findings contribute to understanding the preservation of ipRGCs in late adults and their potential as a biomarker for early ocular degenerative diseases. Methods Female and male C57BL/6J mice were used to assess the behavioral consequences of aging to mature and old adults, including pupillary light reflex, light aversion, visual acuity, and contrast sensitivity. Immunohistochemistry on retinal wholemounts from these mice was then conducted to evaluate ipRGC dendritic morphology in the ganglion cell layer (GCL) and inner nuclear layer (INL). Results Morphological analysis showed that ipRGC dendritic field complexity was remarkably stable through 12 months old of age. Similarly, the pupillary light reflex, visual acuity, and contrast sensitivity were stable in mature and old adults. Although alterations were observed in ipRGC-independent light aversion distinct from the pupillary light reflex, aged wild-type mice continuously showed enhanced light aversion with dilation. No effect of sex was observed in any tests. Conclusions The preservation of both ipRGC morphology and function highlights the potential of ipRGC-mediated function as a valuable biomarker for ocular diseases characterized by early ipRGC loss. The consistent stability of ipRGCs in mature and old adult mice suggests that detected changes in ipRGC-mediated functions could serve as early indicators or diagnostic tools for early-onset conditions such as Alzheimer's disease, Parkinson's disease, and diabetes, where ipRGC loss has been documented.

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Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock in Drosophila

Cavieres-Lepe,

Amini,

Zabel

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks impose daily periodicities to behavior, physiology, and metabolism. This control is mediated by a central clock and by peripheral clocks, which are synchronized to provide the organism with a unified time through mechanisms that are not fully understood. Here, we characterized in Drosophila the cellular and molecular mechanisms involved in coupling the central clock and the peripheral clock located in the prothoracic gland (PG), which together control the circadian rhythm of emergence of adult flies. The time signal from central clock neurons is transmitted via small neuropeptide F (sNPF) to neurons that produce the neuropeptide Prothoracicotropic Hormone (PTTH), which is then translated into daily oscillations of Ca 2+ concentration and PTTH levels. PTTH signaling is required at the end of metamorphosis and transmits time information to the PG through changes in the expression of the PTTH receptor tyrosine kinase (RTK), TORSO, and of ERK phosphorylation, a key component of PTTH transduction. In addition to PTTH, we demonstrate that signaling mediated by other RTKs contributes to the rhythmicity of emergence. Interestingly, the ligand to one of these receptors (Pvf2) plays an autocrine role in the PG, which may explain why both central brain and PG clocks are required for the circadian gating of emergence. Our findings show that the coupling between the central and the PG clock is unexpectedly complex and involves several RTKs that act in concert and could serve as a paradigm to understand how circadian clocks are coordinated.

show abstract

Circuit analysis reveals a neural pathway for light avoidance in Drosophila larvae

Cited by 4 publications

References 37 publications

Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock inDrosophila

Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock inDrosophila

Preservation of Intrinsically Photosensitive Retinal Ganglion Cells (ipRGCs) in Late Adult Mice: Implications as a Potential Biomarker for Early Onset Ocular Degenerative Diseases

Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock in Drosophila

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