CircTRRAP (hsa_circ_0081234) participates in prostate cancer progression and glycolysis by HOXA1 via functioning as a miR-515-5p sponge

Abstract

Dysregulated circular RNAs (circRNAs) are implicated in prostate cancer (PCa) progression. Hsa_circ_0081234 (circTRRAP) has been revealed as a facilitator in PCa, but the mechanisms associated with circTRRAP in PCa progression are largely unclear. The present study was to explore the regulatory mechanism of circTRRAP-mediated PCa progression. A total of 50 PCa tissues and normal tissues were collected. RNA levels of circTRRAP, microRNA (miR)-515-5p and homeobox A1 (HOXA1) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell viability, proliferation, migration, and invasion were estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, 5-ethynyl-2ʹ-deoxyuridine (EdU) and transwell assays, respectively. Cell glycolysis was assessed by measuring glucose uptake and lactate production. The target interaction between circTRRAP or HOXA1 and miR-515-5p was investigated by the dual-luciferase reporter assay. We observed the overt upregulaiton of circTRRAP in PCa samples and cells. Silencing of circTRRAP lowered tumor growth in vivo and restrained PCa cell viability, proliferation, migration, invasion, and glycolysis in vitro. miR-515-5p was negatively regulated by circTRRAP and its deficiency reversed the inhibiting effects of circTRRAP knockdown on PCa cell malignancy and glycolysis. HOXA1 was confirmed as a miR-515-5p target and miR-515-5p overexpression lessened PCa cell malignancy and glycolysis by decreasing HOXA1 expression. Importantly, circTRRAP mediated HOXA1 expression by functioning as a miR-515-5p sponge. In conclusion, circTRRAP took part in PCa progression and glycolysis through mediating the miR-515-5p/HOXA1 axis, suggesting that circTRRAP can serve as a potential therapeutic target for PCa patients.

Exploring the impact of circRNAs on cancer glycolysis: Insights into tumor progression and therapeutic strategies

“…Guan et al [ 301 ] studied the pathophysiological processes underlying CircTRRAP in PCa development. A recent study found that circTRRAP was increased in PCa specimens with spinal metastasis.…”

“…CircTRRAP enhances PCa progression and plays a role in glycolysis in PCa cells. It enhances the ability of PCa cells to invade and glycolysis via the miR-515-5p/HOXA1 pathway [ 301 ]. Silencing circTRRAP reduced glucose and lactate production uptake in PCa cells, suggesting circTRRAP's involvement in glycolysis in PCa [ 301 ].…”

“…It enhances the ability of PCa cells to invade and glycolysis via the miR-515-5p/HOXA1 pathway [ 301 ]. Silencing circTRRAP reduced glucose and lactate production uptake in PCa cells, suggesting circTRRAP's involvement in glycolysis in PCa [ 301 ]. Li and colleagues examined the potential connection between circTRRAP and miR-515-5p [ 301 ].…”

“…Silencing circTRRAP reduced glucose and lactate production uptake in PCa cells, suggesting circTRRAP's involvement in glycolysis in PCa [ 301 ]. Li and colleagues examined the potential connection between circTRRAP and miR-515-5p [ 301 ]. It has been discovered that MiR-515-5p acts as a suppressor in several different cancer types [ 140 , 303 ].…”

“…In PCa, overexpression of circ-0057553 enhanced tumor cell aerobic glycolysis, invasion, and motility through down-regulation of miR-515-5p [ 304 ]. Guan et al [ 301 ] discovered that miR-515-5p was reduced in prostate cancer tissues and cells. Knocking down MiR-515-5p restored the inhibitory effects of circTRRAP silencing on carcinogenesis and glycolysis in PCa cells.…”