circSMAD2 inhibits the epithelial&amp;ndash;mesenchymal transition by targeting miR-629 in hepatocellular carcinoma

Zhang, Xianwei; Luo, Ping; Wei, Jing; Zhou, Hu; Liang, Chunzi; Tu, Jiancheng

doi:10.2147/ott.s158008

Cited by 65 publications

(40 citation statements)

References 28 publications

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“…For example, circMTo1 suppresses Hcc progression by acting as a sponge of mir-9 (25). circSMad2 can inhibit the epithelial-mesenchymal transition via targeting mir-629 in Hcc (43). circ_0067934 promotes tumor metastasis and growth in Hcc via the inhibition of mir-1324 (44).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma

Yang

et al. 2020

Mol Med Report

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Hepatocellular carcinoma (Hcc) has become a major cause of cancer-related mortality worldwide. circular rnas (circrnas) are non-coding rnas that serve important roles in multiple cancers. However, the role of circrnas in Hcc remains largely unknown. in the present study, a circrna microarray dataset of Hcc samples, GSe97332, was downloaded from the gene expression omnibus database. Following data preprocessing, differentially expressed circrnas between Hcc tissues and normal tissues were determined using Geo2r. The circrna-mirna interactions were predicted by the miranda database. The mirTarbase database was used to search for target genes of the mirnas. a circrna-mirna-mrna network was constructed using cytoscape based on the obtained circrna, mirna and mrna. in this network, the upregulated circrna hsa_circrna_100084 was found to be involved in a competing endogenous relationship of hsa_ circrna_100084-hsa-mir-23a-5p-insulin-like growth factor 2 (IGF2). The differential expression of hsa_circrna_100084, hsa-mir-23a-5p and IGF2 in Hcc tissues and liver cancer cells was validated by reverse transcription-quantitative Pcr. additionally, the interactions between hsa-mir-23a-5p with hsa_circrna_100084 and IGF2 were validated by dual-luciferase reporter assays. Knocking down hsa_circrna_100084 inhibited the proliferation, migration and invasion of liver cancer cells, while the simultaneous overexpression of IGF2 reversed the effects of hsa_circrna_100084 knockdown. The results show that hsa_circrna_100084 could promote the expression of IGF2 by acting as a sponge of hsa-mir-23a-5p in liver cancer cells.

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Section: Discussionmentioning

confidence: 99%

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma

Yang

et al. 2020

Mol Med Report

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“…In HCC tissues, circRNA SMAD2 (circSMAD2) expression was found to be decreased compared to adjacent normal tissues. 61 The degree of differentiation of HCC tissues was highly influenced by circSMAD2. Overexpression of circSMAD2 inhibited EMT, invasiveness, and migration of HCC cells.…”

Section: Suppressors In Hccmentioning

confidence: 99%

Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

Shang

Adzika

et al. 2019

Cancer Medicine

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At the heart of hepatocellular carcinoma (HCC) lies disruption of signaling pathways at the level of molecules, genes, and cells. Non‐coding RNAs (ncRNAs) have been implicated in the disease progression of HCC. For instance, dysregulated expression of circular RNAs (circRNAs) has been observed in patients with HCC. As such, these RNAs are potential therapeutic targets and diagnostic markers for HCC. Long non‐coding RNAs (lncRNAs), a type of ncRNA, have also been recognized to participate in the initiation and progression of HCC. Transforming growth factor‐beta (TGF‐β) is another element which is now recognized to play crucial roles in HCC. It has been implicated in many biological processes such as survival, immune surveillance, and cell proliferation. In HCC, TGF‐β promotes disease progression by two mechanisms: an intrinsic signaling pathway and the extrinsic pathway. Through these pathways, it modulates various microenvironment factors such as inflammatory mediators and fibroblasts. An interesting yet‐to‐be resolved concept is whether the HCC‐promoting role of TGF‐β pathways is limited to a subset of HCC patients or it is involved in the whole process of HCC development. This review summarizes recent advancements to highlight the roles of circRNAs, lncRNAs, and TGF‐β in HCC.

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“…Growing evidence demonstrates that circRNAs usually regulate tumor progression and metastasis by affecting EMT 37,38 . In our study, knocking down of circRNA_100290 induced altered expression of several EMT markers, accompanied by the release of miR-29b-3p and blocking of ITGA11.…”

Section: Discussionmentioning

confidence: 99%

EIF4A3-Mediated CircRNA_100290 Promotes GC Cell Proliferation, Invasion and EMT via miR-29b-3p/ITGA11 Axis

Wang¹,

Sun²,

Li³

et al. 2020

Preprint

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Background Circular RNA (circRNA) has been reported as an important regulator in the development and progression of various carcinomas. However, the role of circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed to investigate the role of circRNA_100290 in GC invasion and metastasis and its possible mechanism.Methods The expression of circRNA_100290 in GC cells and tissues were examined using quantitative real-time polymerase chain reaction (qRT-PCR). The role of circRNA_100290 in cell proliferation, migration, and invasion was evaluated on AGS and HGC-27 cell lines in vitro. Bioinformatics tools, dual-luciferase reporter assay, Western blot assay and qRT-PCR were used to explore the downstream pathways of circRNA_100290. The mechanism underlying the regulation of the expression of circRNA_100290 was explored using RNA immunoprecipitation, qRT-PCR, and Western blot assays.Results The expression of circRNA_100290 was found significantly upregulated in GC cells and 102 GC tissues, high expression of circRNA_100290 in GC was closely related to Borrmann’s types, lymph node metastasis and tumor-node-metastasis staging. In vitro, knockdown of circRNA_100290 in AGS and HGC-27 cells significantly inhibited cell proliferation, migration, and invasion. Mechanistically, dual-luciferase reporter assay confirmed a direct binding between circRNA_100290 and miR-29b-3p, which targets ITGA11, an oncogene which is closely related to epithelial–mesenchymal transition (EMT). In addition, EIF4A3, one of RNA binding proteins (RBPs), could inhibit the formation of circRNA_100290 via enriching flanking sites of circRNA_100290. Low expression of EIF4A3 in GC was related to a worse prognosis.Conclusions Elevated circRNA_100290 in GC promotes cell proliferation, invasion and EMT via miR-29b-3p/ITGA11 axi and might be regulated by EIF4A3. CircRNA_100290 might be a promising biomarker and target for GC therapy.

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circSMAD2 inhibits the epithelial–mesenchymal transition by targeting miR-629 in hepatocellular carcinoma

Cited by 65 publications

References 28 publications

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma

Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma

EIF4A3-Mediated CircRNA_100290 Promotes GC Cell Proliferation, Invasion and EMT via miR-29b-3p/ITGA11 Axis

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