CircSETBP1 Acts as a MiR-149-5p Sponge to Promote Intramuscular Fat Deposition by Regulating CRTCs

Abstract

Circular RNAs (circRNAs) appear to be crucial in the process of adipogenesis according to mounting data. CircSETBP1 is a newly discovered circRNA associated with adipogenesis. Sequencing verification and RNase R treatment have confirmed the circular nature of circSETBP1 in porcine tissue. The precise function and mechanism of circSETBP1 in adipocyte biology are still unclear. Cell counting kit-8 (CCK8), Oil red O staining, and quantitative real-time polymerase chain reaction (qRT-PCR) were employed in this investigation to reveal the functions of circSETBP1 and miR-149-5p in the growth and development of porcine intramuscular (IM) preadipocytes. CircSETBP1 overexpression accelerated cell differentiation while reducing cell proliferation. The opposite outcome was produced by overexpressing miR-149-5p. Meanwhile, circSETBP1 down-regulated the expression of miR-149-5p and miR-149-5p restrained the expression of CRTC1/CRTC2. CircSETBP1 was directly targeted by miR-149-5p, and CRTC1/CRTC2 were the target genes of miR-149-5p using bioinformatic analysis, the dual-Luciferase reporter system, and qRT-PCR. In conclusion, circSETBP1 controls the proliferation and differentiation of porcine IM preadipocytes and 3T3-L1 cells by regulating the miR-149-5p/CRTCs axis. The results of this study not only illuminate the molecular mechanism of circSETBP1/miR-149-5p involved in the deposition of porcine intramuscular fat (IMF), but they also provide a significant theoretical reference for raising quality of pork.

CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/MEF2C axis

“… 12 In a separate study, it was revealed that CircSETBP1 expedites the differentiation of intramuscular preadipocytes and 3T3-L1 cells by modulating the miR-149-5p/CRTCs axis, consequently curtailing cell proliferation. 30 Through gain or loss-of-function experiments, we have confirmed that circMEF2C(2, 3) exerts a dual regulatory role in porcine intramuscular preadipocytes. Specifically, we observed that circMEF2C(2, 3) enhances cell proliferation while concurrently inhibiting the differentiation of these cells into mature adipocytes.…”

Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics

“…Porcine IM preadipocytes were isolated from 3-day-old Queshan Black piglets by using the published method [ 42 ]. The improved Eagle medium contains 89% Durbeko (Gibco, Carlsbad, USA), 10% FBS (Gibco, Carlsbad, USA), and 1% penicillin-streptomycin (Solarbio, Beijing, China) growth medium to cultivate porcine IM preadipocytes.…”

“…MiR-149 controls non-alcoholic fatty liver disease by targeting FGF-21 [ 40 ]; it also inhibits the ATF6 pathway to reduce inflammation and apoptosis in nonalcoholic fatty liver disease brought on by endoplasmic reticulum stress [ 41 ]. According to Liu et al, miR-149-5p reduces the differentiation of porcine IM preadipocytes and 3T3-L1 cells while promoting their growth [ 42 ]. However, it is still unclear how miR-149-5p influences the development of porcine IM preadipocyte.…”

Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics

“…Porcine IM preadipocytes were isolated from 3-day-old Queshan black piglets using the published method [43]. The improved Eagle medium contains 89% Durbeko (Gibco, Carlsbad, USA), 10% FBS (Gibco, Carlsbad, USA), and 1% penicillin-streptomycin (Sulaibao, Beijing, China) growth medium to cultivate porcine IM preadipocytes.…”

“…MiR-149 controls non-alcoholic fatty liver disease by targeting FGF-21 [41]; it also inhibits the ATF6 pathway to reduce in ammation and apoptosis in nonalcoholic fatty liver disease brought on by endoplasmic reticulum stress [42]. According to Liu et al, miR-149-5p reduced the differentiation of porcine IM preadipocytes and 3T3-L1 cells while promoting their growth [43]. However, the exact way by which miR-149-5p affects porcine IM preadipocyte development is unknown.…”