CircRNAs as Potential Biomarkers in Gastrointestinal Tract Tumors: Opportunities and Challenges

Liu, Cuixia; Liu, Geoffrey; Cao, Bole; Zhou, Zhenping; Yang, Guangyao; Zhou, Peng

doi:10.7754/clin.lab.2017.170731

Cited by 2 publications

(3 citation statements)

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“…72 fIGURE 1. miRNA expression in the most common cardiovascular diseases (CVDs). 56,[58][59][60][61][62][63][64][65][66] Moreover, numerous studies demonstrated that the activity of NF-κB has important implications on the he- in CVDs. From a molecular point of view, in the activation of TLR4 intervenes the myeloid differentiation factor 81 (MyD88), which is responsible of the connection between Toll/Interleukin-1 (Toll/IL-1) receptor (TIR) and TLR4.…”

Section: Mirnas and Acute Myocardial Infarctionmentioning

confidence: 99%

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Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Buzaș

Rogobete

Popovici

et al. 2018

Journal of Cardiovascular Emergencies

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Worldwide, cardiovascular diseases (CVDs) represent one of the main causes of morbidity and mortality, and acute coronary syndromes are responsible for a large number of sudden cardiac deaths. One of the main challenges that still exist in this area is represented by the early detection and targeted monitoring of the pathophysiology involved in CVDs. During the last couple of years, researchers have highlighted the importance of molecular and epigenetic mechanisms involved in the initiation and augmentation of CVDs, culminating in their most severe form represented by acute myocardial infarction. One of the most studied molecular factors involved in this type of pathology is represented by nuclear transcription factor kappa B (NF-κB), as well as the involvement of microRNAs (miRNAs). It has been suggested that miRNAs can also be involved in the complex process of atheromatous plaque vulnerabilization that leads to an acute cardiac event. In this review paper, we describe the most important molecular mechanisms involved in the pathogenesis of CVDs and atheromatous plaque progression and vulnerabilization, which include molecular mechanisms dependent on NF-κB. For this paper, we used international databases (PubMed and Scopus). The keywords used for the search were “miRNAs biomarkers”, “miRNAs in cardiovascular disease”, “NF-κB in cardiovascular disease”, “molecular mechanism in cardiovascular disease”, and “myocardial NF-κB mechanisms”. Numerous molecular reactions that have NF-κB as a trigger are involved in the pathogenesis of CVDs. Moreover, miRNAs play an important role in initiating and aggravating certain segments of CVDs. Therefore, miRNAs can be used as biomarkers for early evaluation of CVDs. Furthermore, in the future, miRNAs could be used as a targeted molecular therapy in order to block certain mechanisms responsible for inducing CVDs and leading to acute cardiovascular events.

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Section: Mirnas and Acute Myocardial Infarctionmentioning

confidence: 99%

“…shows the most important changes in miRNA expression in the most common cardiovascular diseases 56,[58][59][60][61][62][63][64][65][66]. Nf-κB, INfLAmmATION, AND ATHEROSCLEROSISAnother important factor involved in the molecular mechanisms that lay at the basis of cardiovascular dis-eases is represented by nuclear factor kappa B (NF-κB) 15.…”

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confidence: 99%

Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Buzaș

Rogobete

Popovici

et al. 2018

Journal of Cardiovascular Emergencies

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“…It has been demonstrated that circRNAs have regulatory roles in the expression of genes by binding to miRNAs, suggesting the important role of circRNA-miRNA-mRNA interaction networks in the occurrence and development of human digestive system tumors, thus highlighting their potentials as diagnostic biomarkers (25). For instance, hsa_circRNA_103809 participates in the modulation of biological functions through the miR-532-3p/FoxO4 axis in colorectal cancer (5).…”

Section: Discussionmentioning

confidence: 99%

hsa_circRNA_001587 upregulates SLC4A4 expression to inhibit migration, invasion, and angiogenesis of pancreatic cancer cells via binding to microRNA-223

Zhang

Tan²,

Zhuang³

et al. 2020

American Journal of Physiology-Gastrointestinal and Liver Physi

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Pancreatic cancer (PC) is a malignant tumor that is difficult to diagnose and treat. Circular RNAs (circRNAs) are biomarkers that may be used to diagnose certain cancers or act as targets for cancer treatment. We aimed to explore the functions of human circular RNA 001587 (hsa_circRNA_001587) on the progression of PC and the underlying mechanism. The expression pattern of hsa_circRNA_001587 and microRNA-223 (miR-223) in PC tissues and cells was determined by RT-qPCR. Dual-luciferase reporter gene assay, RNA-pull down, Argonaute 2 (AGO2) immunoprecipitation assay, and Northern blot analysis were applied to verify the binding relationships among hsa_circRNA_001587, miR-223 and solute carrier family 4 member 4 (SLC4A4). Further analysis of their roles was performed in PC cell line PANC-1. Moreover, we either downregulated or upregulated the expression of hsa_circRNA_001587, miR-223 and SLC4A4 by transfection in vitro. A mouse xenograft model of PC cells was established to evaluate tumor growth in vivo. Hsa_circRNA_001587 was poorly expressed but miR-223 was highly expressed in PC tissues and cell lines. Up-regulation of hsa_circRNA_001587 downregulated the expression of MMP-2, MMP-9, MCM2 and VEGF, and decreased the proliferation, migration, invasion, angiogenic and tumorigenic abilities of PC cells. MiR-223, which can bind with hsa_circRNA_001587, reversed the effects of hsa_circRNA_001587 on PC cells. In addition, SLC4A4 was identified as a target of miR-223, and its knockdown could counteract the regulatory effects of overexpressed hsa_circRNA_001587 or inhibited miR-223 expression on PC cells. Therefore, hsa_circRNA_001587 inhibits PC cell migration, invasion, angiogenesis and tumorigenesis by impairing miR-223-mediated SLC4A4 inhibition.

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CircRNAs as Potential Biomarkers in Gastrointestinal Tract Tumors: Opportunities and Challenges

Cited by 2 publications

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Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

Nuclear Transcription Factor Kappa B (NF-кB) and Molecular Damage Mechanisms in Acute Cardiovascular Diseases. A Review

hsa_circRNA_001587 upregulates SLC4A4 expression to inhibit migration, invasion, and angiogenesis of pancreatic cancer cells via binding to microRNA-223

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