CircRNAs: a new target for the diagnosis and treatment of digestive system neoplasms

Li, Jie; Xu, Qiang; Huang, Zegui; Mao, Ning; Lin, Zhitao; Cheng, Long; Sun, Bei; Wang, Gang

doi:10.1038/s41419-021-03495-0

Cited by 43 publications

(33 citation statements)

References 91 publications

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“…The proliferation and migration of endometrial tissue outside of the uterus is a vital character of endometriosis; whereas the exact pathological progression remains largely unknown [20]. CircRNAs has been recognized as important molecules involving in various biological processes and its abnormality may contribute to many diseases [3]. Studies have showed abnormal expression of circRNAs in endometriosis and indicated their diagnosis value in in discerning diseases at earlier time [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…Circular RNAs (circRNAs) are a class of endogenous RNAs with closed loop structure which ascertained the more stability than linear RNAs. With high abundance and speci c expression in different tissues or developmental stages, circRNAs have been revealed to participate in the regulation of biological and pathological processes such as cell proliferation, apoptosis, invasion, angiogenesis and epithelialmesenchymal transformation, by acting as microRNA (miRNA) sponges [3,4]. Evidences have demonstrated aberrant circRNA expression in endometriosis [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Yang

Ban

Zhang

et al. 2021

Preprint

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Background: Endometriosis is a prevalent gynecologic disease, affecting up to 10% of women at reproductive age and approximately 50% of women with infertility. The function of circRNAs in various diseases has been highlighted. Dysregulated expression of circRNAs in endometriosis has been reported and circ_0000673 was significantly deregulated. However, its explicit role in pathogenesis of endometriosis is yet to be identified. Methods: circ_0000673 expression was detected in paried ectopic and eutopic endometrium using qPCR and fluorescent in situ hybridization. Knockdown of circ_0000673 in eutopic and normal endometrial stromal cells were done by transfection with lentivirus vectors. The proliferation activity of endometrial stromal cells was evaluated by CCK-8 assay and colony formation assay, while the migration capacity was valued by wound healing assay. PTEN, PI3K and p-AKT were detected by qPCR and western blotting. Dual luciferase assay was performed to assess the bonding between circ_0000673, PTEN and miR-616-3p.Results: The expression of circ_0000673 was reduced in ectopic endometrium. Knockdown of circ_0000673 significantly induced eutopic and normal endometrial cell proliferation and migration. Bioinformatic analysis predicted that circ_0000673 might sponge miR-616-3p. The effect of circ_0000673 knockdown could be recovered by miR-616-3p inhibitor and enhanced by miR-616-3p mimics. Meanwhile, qPCR and western blotting showed that circ_0000673 knockdown inhibited the expression of PTEN, and subsequently activated PI3K and p-Akt. Furthermore, PTEN was confirmed to be a target of miR-616-3p. Conclusion: The results demonstrated that deregulated expression of circ_0000673 could promote endometriosis progression via sponging miR-616-3p and further regulating PTEN.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Yang

Ban

Zhang

et al. 2021

Preprint

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“…Circular RNAs (circRNAs) are a class of endogenous RNAs with a closed loop structure that makes them more stable than linear RNAs. circRNAs are highly abundant and specifically expressed in different tissues and developmental stages and have been found to participate in the regulation of biological and pathological processes such as cell proliferation, apoptosis, invasion, angiogenesis, and the epithelial-mesenchymal transition (EMT), by acting as microRNA (miRNA) sponges [3][4][5]. Previous studies have found aberrant circRNA expression in endometriosis [6,7].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

Yang¹,

Ban²,

Zhang³

et al. 2021

Int. J. Med. Sci.

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Endometriosis is a common gynecological disease, affecting up to 10% of women of reproductive age and approximately 50% of women with infertility. Circular RNAs (circRNAs) have been shown to be involved in a number of diseases. Dysregulated expression of circRNAs in endometriosis has been reported, and circ_0000673 was significantly downregulated. However, the details of its role in the pathogenesis of endometriosis are still poorly understood. We investigated the location and effects of the downregulation of circ_0000673 in endometriosis. We demonstrated that knockdown of circ_0000673 significantly increased the proliferation and migration of eutopic and normal endometrial cells. Bioinformatics analysis predicted that circ_0000673 might act as a sponge for miR-616-3p. We found that the effect of circ_0000673 knockdown could be recovered by miR-616-3p inhibitor and enhanced by miR-616-3p mimics. qPCR and western blot assays showed that circ_0000673 knockdown could decrease the expression of PTEN and increase the expression of PI3K and p-AKT. PTEN was confirmed to be a target of miR-616-3p. These results demonstrated that the downregulation of circ_0000673 could promote the progression of endometriosis by inactivating PTEN via the deregulation of miR-616-3p.

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“…Circular RNAs (circRNAs) are a type of endogenous non-coding RNA that do not have a 5'-cap or a 3'-polyA tail and are implicated in a variety of biological functions [4][5]. Recently, RNA-sequences revealed that circRNAs are involved in diagnosis, prognosis, development, and drug resistance in tumors.…”

Section: Introductionmentioning

confidence: 99%

CircTP63 Promotes Cell Proliferation and Invasion by Regulating EZH2 Via Sponging miR-217 in Gallbladder Cancer

Wang

Tong

et al. 2021

Preprint

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Background: Gallbladder cancer (GBC) is the most common biliary tract malignancy and has a poor prognosis in patients with GBC. CircRNA TP63 (circTP63) has been implicated in some tumor proliferation and invasion in some tumors. The study aims to investigate the clinical significance and functional role of circTP63 in GBC.Methods: The expression of circTP63 in GBC was detected by qRT-PCR and the association between circTP63 expression and prognosis of GBC patients was analyzed. CCK8 assay, flow cytometry analysis, transwell assay and in vivo studies were used to evaluated the cell proliferation and invasion after circTP63 knockdown in GBC cells. Luciferase reporter assays and RNA pull-down assay were used to determine the correlation between circTP63 and miR-217. Besides, western blot analysis was also performed.Results: In the present study, we showed that circTP63 expression was upregulated in GBC tissues and cells. Higher circTP63 expression was associated with lymph node metastasis and short overall survival (OS) in patients with GBC. In vitro, knockdown of circTP63 inhibited cell proliferation, cell cycle progression, migration and invasion in GBC. Besides, we demonstrated that knockdown of circTP63 inhibited GBC cell EMT process. In vivo, knockdown of circTP63 inhibited tumor growth in GBC. Mechanistically, we demonstrated that circTP63 competitively bind to miR-217 and promoted EZH2 expression and finally facilitated tumor progression.Conclusions: Our findings demonstrated that circTP63 sponge miR-217 and regulated EZH2 expression and finally facilitates tumor progression. Thus, targeting circTP63 may be a therapeutic strategy for the treatment of GBC.

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CircRNAs: a new target for the diagnosis and treatment of digestive system neoplasms

Cited by 43 publications

References 91 publications

Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Downregulation of Circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis Via Suppressing PTEN

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis

CircTP63 Promotes Cell Proliferation and Invasion by Regulating EZH2 Via Sponging miR-217 in Gallbladder Cancer

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