CircRNA_0058063 functions as a ceRNA in bladder cancer progression via targeting miR-486-3p/FOXP4 axis

Abstract

Emerging evidence has uncovered critical regulatory roles of circular RNAs (circRNAs) function as dynamic scaffolding molecules in tumorigenesis and progression. However, the aberrant expression and clinical significance of hsa_circ_0058063 (circRNA_0058063) in bladder cancer (BC) remain poorly understood. circRNA expression was analyzed via a microarray in cancerous tissue and non-carcinoma tissues. Luciferase reporter assays and RNA immunoprecipitation (RIP) were both conducted to uncover the function of circRNA_0058063 in BC. circRNA_0058063 was overexpressed in BC tissues compared with adjacent normal tissues. Knockdown of circRNA_0058063 dramatically decreased cell proliferation and invasion, and promoted apoptosis in 5637 and BIU-87 cell lines. Furthermore, mechanistic investigations showed that circRNA_0058063 and FOXP4 could directly bind to miR-486-3p, demonstrating that circRNA_0058063 regulated FOXP4 expression by competitively binding to miR-486-3p. Taken together, circRNA_0058063 functions by sponging miR-486-3p in BC progression, which could act as a new biomarker and further developed to be a therapeutic target in BC.

miRNAs derived from circulating small extracellular vesicles as diagnostic biomarkers for nasopharyngeal carcinoma

“…40,41 Some studies have reported that miR-486-3p promotes cell invasion and tumor progression through the activation of cancerassociated signaling pathways in NPC and other various types of cancer. 42,43 Additionally, miR-486-3p is a member of the regulatory network associated with the pathophysiology of NPC. 44 Although the role of miR-486-5p in NPC has not been reported previously, it may serve as a biomarker for the recurrence of oral squamous cell carcinoma after operation.…”

<p>The Biogenesis, Functions, and Roles of circRNAs in Bladder Cancer</p>

“…Liang et al confirmed that circRNA_0058063 was augmented in BCa tissues compared with normal tissues and high expression of circRNA_0058063 was significantly correlated with higher pathological stage and increased likelihood of lymphatic metastasis. 80…”

Silencing Circ_0058063 Enhances Proliferation and Inhibits Apoptosis in PCOS Ovarian Granulosa Cells

“…Circ_0058063 is only been studied in esophageal squamous-cell carcinomas and bladder cancer [10,15,16]. In the progression of bladder cancer, circRNA_0058063 serves as a ceRNA via sponging miR-486-3p/FOXP4 and miR-145-5p/CDK6 axis [10,16].Circ_0058063 stimulates glucose-uptake and increases the expression of GLUT1 in esophageal squamous-cell carcinomas [15].…”