circRNA-miRNA cross-talk in the transition from paroxysmal to permanent atrial fibrillation

Costa, Marina C.; Cortez‐Dias, Nuno; Gabriel, André F; Sousa, João de; Fiúza, Manuela; Gallego, Javier; Nobre, Ângelo; Pinto, Fausto J.; Enguita, Francisco J.

doi:10.1016/j.ijcard.2019.04.072

Cited by 23 publications

(19 citation statements)

References 15 publications

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“…Bao et al described FMRP isoform 1, in rats, as an essential protection factor and a novel potential biomarker in the cardiovascular system [47]. The participation of circRNAs in regulatory networks involving competing-endogenous RNA interactions by sequestering miRNAs has been characterized recently in cardiovascular pathologies [48,49]. From the set of miRNAs that could be sponged by the circRNAs that we considered, we found signi cant enrichment in the regulation of focal adhesion and actin cytoskeleton.…”

Section: Discussionmentioning

confidence: 72%

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Costa

Calderón‐Domínguez

Mangas

et al. 2021

Preprint

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Background: Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing, -involving genetics, imaging, or cardiovascular techniques-, makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case-control multicentric study. Methods: We enrolled 130 subjects: healthy controls (n=20), idiopathic DCM (n=30), ischemic DCM (n=20) and familial DCM patients which included pathogen variants of i) LMNA gene (n=30) and ii) BCL2-associated athanogene 3 (BAG3) gene (n=30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools. Results: Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM; and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs. Conclusions: The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker.

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Section: Discussionmentioning

confidence: 72%

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Costa

Calderón‐Domínguez

Mangas

et al. 2021

Preprint

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“…We have found that there were some researches exploring the expression characterization of circRNA in atrial fibrillation. Zhang et al (28) mentioned the expression profiles of circRNA in non-valvular persistent AF of four paired patient samples and found 296 differentially expressed circRNAs; Hu et al (29) described the circRNAs in AF patients with rheumatic heart disease; Marina et al (30) figured out a circRNA-miRNA cross-talk between paroxysmal and permanent AF. In this study, we revealed the differentially expressed circRNAs between VHD patients with or without AF in order to find the possible molecular targets during the progress of AF in the condition of atrial structure remodeling.…”

Section: Discussionmentioning

confidence: 99%

Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases

Zhu

Tang

Chong

et al. 2020

Front. Cardiovasc. Med.

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Circular RNAs (circRNA) are involved in a variety of human heart diseases, however, circRNA expression profiles and circRNA-miRNA-mRNA regulatory network in human atrial fibrillation (AF) especially with valvular heart diseases (VHD) remain poorly understood. A high-throughput RNA sequencing was used to investigate the differentially expressed circRNAs in left atrial appendage from VHD patients with or without persistent AF. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to predict the potential functions of the host genes of differentially expressed circRNA and their downstream targets. CircRNA–miRNA-mRNA regulatory network was constructed to identify mechanisms underlying circRNAs. qRT-PCR and sanger sequencing were further performed to validate the results. Compared with sinus rhythm (SR) patients, there were 3094 upregulated and 4472 downregulated circRNAs in AF patients respectively. The expression of 10 most differentially expressed circRNAs (circ 255-ITGA7, circ 418-KCNN2, circ 13913-MIB1, circ 44670-BARD1, circ 44782-LAMA2, circ 81906-RYR2, circ 35880-ANO5, circ 22249-TNNI3K, circ 3136-TNNI3K, circ 56186-TNNI3K) between SR and persistent AF patients were verified by qRT-PCR. In addition, specific back-splicing sites of these circRNAs was confirmed by sanger sequencing. GO and KEGG pathway analysis indicated that cAMP signal pathway and Wnt signal pathway might play important role in the development of AF in VHD patients, which might be affected by circRNAs. This study provided a preliminary landscape of circRNAs expression profiles which are involved in persistent AF due to VHD, and established the possibility for future related researches in this field.

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“…Apoptosis of cardiomyocytes has been shown to be directly augmented by the action of miR-122, which is overexpressed during AF [159]. In the left-atria of patients, a global downregulation of miRNAs (112 candidates) has also been observed in the time window between the paroxysmal and the permanent condition, overlapping in time with the expression of 40 circular (circ)RNAs, suggesting a cross-talk network between both families of ncRNAs [160].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Functional genomics and epigenomics of atrial fibrillation

Victorino

Alvarez-Franco

Manzanares

2021

Journal of Molecular and Cellular Cardiology

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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circRNA-miRNA cross-talk in the transition from paroxysmal to permanent atrial fibrillation

Cited by 23 publications

References 15 publications

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Expression Profiles of Circular RNA in Human Atrial Fibrillation With Valvular Heart Diseases

Functional genomics and epigenomics of atrial fibrillation

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