CircRNA in cancer: Fundamental mechanism and clinical potential

Chen, Liang; Ge, Shengfang

doi:10.1016/j.canlet.2021.02.004

Cited by 255 publications

(212 citation statements)

References 96 publications

Supporting

Mentioning

210

Contrasting

Unclassified

Order By: Relevance

“…Although circRNAs is defined as a class of rubbish during the splicing process since it was firstly discovered, nowadays it has been considered as a pivotal regulator to participate in diverse physiological and pathological processes [ 11 ]. Mounting evidence shows that circRNAs play vital roles in carcinogenesis and cancer progression [ 12 – 15 ]. For example, circRNAs could sponge miRNAs to regulate expression of target gene post-transcriptionally, such as circAKT3 [ 16 ] and circSDHC [ 17 ] in RCC.…”

Section: Introductionmentioning

confidence: 99%

CircMET promotes tumor proliferation by enhancing CDKN2A mRNA decay and upregulating SMAD3

et al. 2022

View full text Add to dashboard Cite

Background Functions of CircMET (hsa_circ_0082002) which is a circular RNA and derived from MET gene remain understood incompletely. In the present study, Xp11.2 translocation/NONO-TFE3 fusion renal cell carcinoma (NONO-TFE3 tRCC) with up-regulated CircMET was employed to investigate its mechanism in cancer progression and post-transcriptional regulation. Methods FISH and real-time PCR were performed to explore the expression and localization circMET in NONO-TFE3 tRCC tissues and cells. The functions of circMET in tRCC were investigated by proliferation analysis, EdU staining, colony and sphere formation assay. The regulatory mechanisms among circMET, CDKN2A and SMAD3 were investigated by luciferase assay, RNA immunoprecipitation, RNA pulldown and targeted RNA demethylation system. Results The expression of circMET was upregulated by NONO-TFE3 fusion in NONO-TFE3 tRCC tissues and cells, and overexpression of circMET significantly promoted the growth of NONO-TFE3 tRCC. Mechanistic studies revealed that circMET was delivered to cytosol by YTHDC1 in N6-methyladenosine (m6A)-depend manner. CircMET enhances mRNA decay of CDKN2A by direct interaction and recruitment of YTHDF2. Meanwhile, circMET competitively absorbed miR-1197 and prevented those from SMAD3 mRNA. Conclusions CircMET promotes the development of NONO-TFE3 tRCC, and the regulation to both CDKN2A and SMAD3 of circMET was revealed. CircMET has the potential to serve as a novel target for the molecular therapy of NONO-TFE3 tRCC as well as the other cancer with high-expressing circMET.

show abstract

Section: Introductionmentioning

confidence: 99%

CircMET promotes tumor proliferation by enhancing CDKN2A mRNA decay and upregulating SMAD3

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Circular RNAs (circRNAs) are an evolutionarily conserved class of ncRNAs that are formed in a circular conformation via noncanonical splicing or backsplicing events [6]. Global expression of circRNAs is differentially altered in ischemic lesion tissue, peripheral blood, and peripheral blood mononuclear cell from AIS patients [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2

Yang

Zhong

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Background. Circular RNA phosphorylase kinase regulatory subunit alpha 2 (circPHKA2; hsa_circ_0090002) has a significantly, specifically different expression in acute ischemic stroke (AIS) patients’ blood. Here, we intended to investigate the role and mechanism of circPHKA2 in oxygen-glucose deprivation- (OGD-) induced stoke model in human brain microvascular endothelial cells (HBMEC). Methods. Expression of circPHKA2, microRNA- (miR-) 574-5p, and superoxide dismutase-2 (SOD2) was detected by quantitative PCR and western blotting. Cell injury was measured by detecting cell proliferation (EdU assay and CCK-8 assay), migration (transwell assay), neovascularization (tube formation assay), apoptosis (flow cytometry and western blotting), endoplasmic reticulum stress (western blotting), and oxidative stress (assay kits). Direct intermolecular interaction was determined by bioinformatics algorithms, dual-luciferase reporter assay, biotin-labelled miRNA capture, and argonaute 2 RNA immunoprecipitation. Results. circPHKA2 was downregulated in AIS patients’ blood in SOD2-correlated manner. Reexpressing circPHKA2 rescued EdU incorporation, cell viability and migration, tube formation, B cell lymphoma-2 (Bcl-2) expression, and SOD activity of OGD-induced HBMEC and alleviate apoptotic rate and levels of Bcl-2-associated protein (Bax), glucose-regulated protein 78 kD (GRP78), C/EBP-homologous protein (CHOP), caspase-12, reactive oxygen species (ROS), and malondialdehyde (MDA). Additionally, blocking SOD2 partially attenuated these roles of circPHKA2 overexpression. Molecularly, circPHKA2 upregulated SOD2 expression via interacting with miR-574-5p, and miR-574-5p could target SOD2. Similarly, allied to neurovascular protection of circPHKA2 was the downregulation of miR-574-5p. Conclusion. circPHKA2 could protect HBMEC against OGD-induced cerebral stroke model via the miR-574-5p/SOD2 axis, suggesting circPHKA2 as a novel and promising candidate in ischemic brain injury.

show abstract

“…Normally circRNAs cannot be flipped, but with deeper research, it was found that exon sequences of some circRNAs can be translated into proteins ( Chen and Shan, 2021 ; Dodbele et al, 2021 ). Some circRNAs contain internal ribosome entry site (IRES) sequences and can bind directly to ribosomes and can be translated in eukaryotic cells ( Abe et al, 2015 ; He L. et al, 2021 ).…”

Section: Biological Functions Of Circular Rnasmentioning

confidence: 99%

Research Progress of circRNAs in Glioblastoma

Guo

Piao

2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are a class of single-stranded covalently closed non-coding RNAs without a 5′ cap structure or 3′ terminal poly (A) tail, which are expressed in a variety of tissues and cells with conserved, stable and specific characteristics. Glioblastoma (GBM) is the most aggressive and lethal tumor in the central nervous system, characterized by high recurrence and mortality rates. The specific expression of circRNAs in GBM has demonstrated their potential to become new biomarkers for the development of GBM. The specific expression of circRNAs in GBM has shown their potential as new biomarkers for GBM cell proliferation, apoptosis, migration and invasion, which provides new ideas for GBM treatment. In this paper, we will review the biological properties and functions of circRNAs and their biological roles and clinical applications in GBM.

show abstract

CircRNA in cancer: Fundamental mechanism and clinical potential

Cited by 255 publications

References 96 publications

CircMET promotes tumor proliferation by enhancing CDKN2A mRNA decay and upregulating SMAD3

CircMET promotes tumor proliferation by enhancing CDKN2A mRNA decay and upregulating SMAD3

Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2

Research Progress of circRNAs in Glioblastoma

Contact Info

Product

Resources

About