circRNA, a novel diagnostic biomarker for coronary heart disease

Abstract

ObjectiveThis study aimed to identify the potential diagnostic biomarkers of coronary heart disease (CHD) from exosome-derived circRNA.MethodsThe microarray data of circRNA derived from the exosomes of patients with CHD and mRNA in acute myocardial infarction was retrieved from exoRBase website and GEO database (GSE61144), respectively, to identify the differentially expressed genes (DEGs). Our findings detected the differentially expressed circRNAs and mRNAs and predicted their correlation with microRNAs using the microRNA target prediction website, thus ascertaining the corresponding circ-microRNA and micro-mRNAs. Then, we performed systematic Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on the differentially expressed mRNA. Protein-Protein Interactions (PPI) of these DEGs were examined using STRING. The receiver operator characteristic (ROC) curve was used to validate the diagnostic efficacy of circRNA in patients with CHD. Finally, the RNAs identified in this study were verified by quantitative real-time polymerase chain reaction (qRT-PCR).ResultsA total of 85 differentially expressed circRNAs (4 up-regulated and 81 down-regulated) were identified by screening the circRNAs in exosome of CHD patients. Based on the prediction data of circRNA, mRNA, and the corresponding microRNA, a ceRNA network was constructed, including 7 circRNA nodes, 5 microRNA nodes, and 2 mRNA nodes. Finally, validated by qRT-PCR testing, we found circRNA0001785, circRNA0000973, circRNA0001741, and circRNA0003922 to be the promising candidate for the effective prediction of CHD. These potential diagnostic markers can provide insight for further research on the occurrence of CHD or even acute coronary syndrome (ACS).

Serum-derived exosomal circENC1 inhibits SMC phenotypic switching to stabilize atherosclerotic plaques by interacting with miR-513a-5p and inhibiting MYH9 degradation

“…These findings strongly suggest that exosomal circENC1 is a suitable biomarker for diagnosing plaque stability. Previous studies have identified exosomal circRNAs, including exosomal circ_0043837 28 and circRNA0001785 29 , as biomarkers for cardiovascular diseases such as left atrial appendage (LAA) stroke and coronary heart disease. These diseases are intricately linked to plaque stability.…”

Exploring the Multifaceted Biologically Relevant Roles of circRNAs: From Regulation, Translation to Biomarkers

“…A recent microarray analysis of exosomes collected from 6 cases of coronary heart disease (CHD) patients and 32 healthy subjects revealed 85 differentially regulated cir-cRNAs, with 4 candidates (circRNA0001785, circRNA0000973, circRNA0001741, and cir-cRNA0003922) being proposed as promising predictive biomarkers for CHD and acute coronary syndrome (ACS) [62]. CircSLC8A1 and circNFIX are another pair of circRNAs relevant to the forensic diagnosis of sudden cardiac death (SCD) and acute ischemic heart disease (IHD).…”

“…Thus far, circRNAs have been revealed to play biologically significant roles in regulating tumorigenesis and metastasis in different types of cancer [37][38][39][40][41], brain development and diseases [42][43][44], bone development and diseases [45][46][47][48], myogenesis and myopathies [49,50], aging [51], and many other diseases and biological processes [52][53][54][55][56][57][58]. Another arena in which circRNAs have recently garnered tremendous levels of functional importance is the regulation of cardiovascular development and diseases [59][60][61][62][63][64].…”