CircRNA-1967 participates in the differentiation of goat SHF-SCs into hair follicle lineage by sponging miR-93-3p to enhance LEF1 expression

Zhu, Ying; Wang, Yanru; Zhao, Junyin; Shen, Jincheng; Wang, Zeying; Bai, Man; Fan, Yixing; Yin, Rong; Mao, Yongjiang; Bai, Wenlin

doi:10.1080/10495398.2021.1975729

Cited by 11 publications

(15 citation statements)

References 42 publications

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“…Moreover, the circRNA-1926 was found to positively regulate the differentiation of SHF stem cells into hair lineages in cashmere goats through the miR-148a/b-3p/CDK19 axis [22]. More recently, the circRNA-1967 was also demonstrated to participate in the differentiation of SHF stem cells into the hair follicle lineage by sponging miR-93-3p, and as a result enhancing LEF1 expression in cashmere goats [23]. These findings suggested that circRNAs might play important regulatory roles in the development of SHFs and cashmere fiber growth in cashmere goats.…”

Section: Introductionmentioning

confidence: 98%

Identification and Molecular Analysis of m6A-circRNAs from Cashmere Goat Reveal Their Integrated Regulatory Network and Putative Functions in Secondary Hair Follicle during Anagen Stage

Hui

Zhu

Shen

et al. 2022

Animals

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N6-methyladenosine (m6A) is the most abundant modification in linear RNA molecules. Over the last few years, interestingly, many circRNA molecules are also found to have extensive m6A modification sites with temporal and spatial specific expression patterns. To date, however, little information is available concerning the expression profiling and functional regulatory characteristics of m6A modified circRNAs (m6A-circRNAs) in secondary hair follicles (SHFs) of cashmere goats. In this study, a total of fifteen m6A-circRNAs were identified and characterized in the skin tissue of cashmere goats. Of these, six m6A-circRNAs were revealed to have significantly higher expression in skin at anagen compared with those at telogen. The constructed ceRNA network indicated a complicated regulatory relationship of the six anagen up-regulated m6A-circRNAs through miRNA mediated pathways. Several signaling pathways implicated in the physiological processes of hair follicles were enriched based on the potential regulatory genes of the six anagen up-regulated m6A-circRNAs, such as TGF-beta, axon guidance, ribosome, and stem cell pluripotency regulatory pathways, suggesting the analyzed m6A-circRNAs might be essentially involved in SHF development and cashmere growth in cashmere goats. Further, we showed that four m6A-circRNAs had highly similar expression trends to their host genes in SHFs of cashmere goats including m6A-circRNA-ZNF638, -TULP4, -DNAJB6, and -CAT. However, the expression patterns of two m6A-circRNAs (m6A-circRNA-STAM2 and -CAAP1) were inconsistent with the linear RNAs from their host genes in the SHFs of cashmere goats. These results provide novel information for eluci-dating the biological function and regulatory characteristics of the m6A-circRNAs in SHF development and cashmere growth in goats.

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Section: Introductionmentioning

confidence: 98%

Identification and Molecular Analysis of m6A-circRNAs from Cashmere Goat Reveal Their Integrated Regulatory Network and Putative Functions in Secondary Hair Follicle during Anagen Stage

Hui

Zhu

Shen

et al. 2022

Animals

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“…Over the past few years, extensive m 6 A modifications sites are also found in many circRNA molecules that play significant roles in a variety of biological cells through m 6 A modification dependent model [11]. To date, a considering number circRNAs have been identified in skin tissue or SHFs of cashmere goat [12], and some of them are demonstrated to positively regulate the A c c e p t e d A r t i c l e differentiation of SHF stem cells into hair follicle lineage, such as circRNA-1926 [13], circRNA-1967 [14] and circRNA-0100 [15]. Moreover, some circRNAs in SHFs of cashmere goats are verified to contain multiple m 6 A modification sites [2].…”

Section: Introductionmentioning

confidence: 99%

N6-Methyladenosine modification (m6A) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats

Yin

Bai

et al. 2023

Anim Biosci

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Objective:The objective of this study was to investigate the effects of N 6 -Methyladenosine modification-circRNA-zinc finger protein 638 (m 6 A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m 6 A modification of ZNF638 was analyzed using Methylation Immunoprecipitation (Me-RIP) with real-time quantitative PCR (RT-qPCR) technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m 6 A dependence were evaluated through the overexpression of circRNA-ZNF638/its m 6 A-deficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′untranslated region (3′-UTR) was analyzed through Dual-luciferase reporter assay. Results:The m 6 A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as, it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m 6 A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHF-stem cells. We further demonstrated that the

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“…In the present study, the results confirmed that FoxO1 was a high-affinitive target of miR-93-3p in OA chondrocytes. Although the results demonstrated that FoxO1 was a target gene of miR-93-3p in OA chondrocytes, a number of previous studies have reported that miR-93-3p has multiple target genes ( 34 , 41 , 42 ). Therefore, whether other miR-93-3p-targeting genes are also involved in maintaining the balance of chondrocyte matrix metabolism should be explored in future studies.…”

Section: Discussionmentioning

confidence: 67%

circPhc3 sponging microRNA‑93‑3p is involved in the regulation of chondrocyte function by mechanical instability in osteoarthritis

et al. 2021

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CircRNA-1967 participates in the differentiation of goat SHF-SCs into hair follicle lineage by sponging miR-93-3p to enhance LEF1 expression

Cited by 11 publications

References 42 publications

Identification and Molecular Analysis of m6A-circRNAs from Cashmere Goat Reveal Their Integrated Regulatory Network and Putative Functions in Secondary Hair Follicle during Anagen Stage

Identification and Molecular Analysis of m6A-circRNAs from Cashmere Goat Reveal Their Integrated Regulatory Network and Putative Functions in Secondary Hair Follicle during Anagen Stage

N6-Methyladenosine modification (m6A) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats

circPhc3 sponging microRNA‑93‑3p is involved in the regulation of chondrocyte function by mechanical instability in osteoarthritis

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