CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells

Li, Xiajing; Zhang, Yiyu; Wang, Ning; Yuan, Zhaohu; Chen, Xiaojie; Chen, Qicong; Deng, Hui; Tong, Xuhui; Chen, Honglin; Duan, Yuyou; Wei, Yaming

doi:10.1631/jzus.b2200048

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…At the same time, interference with circRNA_0092516 reversed these effects, suggesting that circRNA_0092516 inhibits chondrocyte proliferation and inflammatory factor release and promotes apoptosis [ 21 ]. In a human myeloid leukemia cell model, circRNA.0007127 associates with miR-513a-5p or CASP8 to reduce apoptosis by inhibiting CASP8 pathway activation [ 22 ]. In diabetic cardiomyopathy (DCM), cyclic RNA cerebellar degeneration-associated protein 1 antisense (Circ-CDR1as) promotes cardiomyocyte apoptosis by activating the Hippo signaling pathway through significant inhibition of mammalian sterile 20-like kinase 1 (MST1) ubiquitination levels [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Circ_PIAS1 Promotes the Apoptosis of ALV-J Infected DF1 Cells by Up-Regulating miR-183

Yang

Qiu

Chen

et al. 2023

Genes

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(1) Background: circRNAs are closed circular molecules with covalent bonds generated by reverse shearing, which have high stability and have different manifestations in different tissues, cells, or physiological conditions and play important roles in various disease processes and physiological processes. In addition, circ_PIAS1 has been screened out and verified, and the bioinformatics analyzed in previous studies. In this study, we investigated the function of circ_PIAS1 and studied its role in ALV-J infection to provide a basis for the role of circRNA in ALV-J infection. (2) Methods: the effect of circ_PIAS1 on apoptosis during ALV-J infection was studied by flow cytometry and detection of apoptotic gene expression, and miR-183 was screened by a biotin-labeled RNA pull-down technique. After overexpression and inhibition of miR-183, the effect of miR-183 on apoptosis in the process of ALV-J infection was studied by flow cytometry and detection of apoptotic gene expression. (3) Results: after overexpression of circ_PIAS1, flow cytometry and apoptotic gene expression showed that circ_PIAS1 promoted apoptosis. The results of RNA pull-down showed that 173 miRNAs could bind to circ_PIAS1, and circ_PIAS1 up-regulated the expression of miR-183. On the other hand, the same results were obtained whether miR-183 was overexpressed or inhibited that miR-183 affected ALV-J infection by promoting cell apoptosis. (4) Conclusions: circ_PIAS1 up-regulated the expression of miR-183 and influenced ALV-J infection by promoting cell apoptosis.

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Section: Discussionmentioning

confidence: 99%