CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‐125a‐3p

Ling, Sunkai; He, Yanru; Li, Xiaoxue; Hu, Mingyue; Ma, Yu; Li, Yuan; Lu, Zipeng; Shen, Shanshan; Kong, Bo; Zou, Xiaoping; Jiang, Kuirong; Huang, Peilin

doi:10.1111/jcmm.15572

Cited by 21 publications

(18 citation statements)

References 26 publications

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“…A similar study also showed that circRHOT1 served as a sponge and bond to miR-125a-3p, upregulating oncogenic E2F3. Knockdown of circRHOT1 significantly suppressed pancreatic cancer cells through the regulation miR-125a-3p/E2F3 axis [115]. These findings suggest the oncogenic effects of circRHOT1.…”

Section: Circrhot1mentioning

confidence: 65%

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Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

Hallak

Philip

et al. 2021

Cancers

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Pancreatic cancer is an aggressive malignance with high mortality. The lack of early diagnosis and effective therapy contributes to the high mortality of this deadly disease. For a long time being, the alterations in coding RNAs have been considered as major targets for diagnosis and treatment of pancreatic cancer. However, with the advances in high-throughput next generation of sequencing more alterations in non-coding RNAs (ncRNAs) have been discovered in different cancers. Further mechanistic studies have demonstrated that ncRNAs such as long noncoding RNAs (lncRNA), circular RNAs (circRNA) and piwi-interacting RNA (piRNA) play vital roles in the regulation of tumorigenesis, tumor progression and prognosis. In recent years, increasing studies have focused on the roles of ncRNAs in the development and progression of pancreatic cancer. Novel findings have demonstrated that lncRNA, circRNA, and piRNA are critically involved in the regulation of gene expression and cellular signal transduction in pancreatic cancer. In this review, we summarize the current knowledge of roles of lncRNA, circRNA, and piRNA in the diagnosis and prognosis of pancreatic cancer, and molecular mechanisms underlying the regulation of these ncRNAs and related signaling in pancreatic cancer therapy. The information provided here will help to find new strategies for better treatment of pancreatic cancer.

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Section: Circrhot1mentioning

confidence: 65%

“…circRHOT1 (hsa_circ_0005397) is highly expressed in pancreatic cancer and is mainly located in the cytoplasm of pancreatic cancer cells [115,116]. Down-regulation of circRHOT1 suppressed pancreatic cell proliferation, invasion, and migration.…”

Section: Circrhot1mentioning

confidence: 99%

Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

Hallak

Philip

et al. 2021

Cancers

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“…In a study by Kojima et al (21), as well as our prior bioinformatics investigations, miR-125a-3p was found to be down-regulated in plasma samples of PC patients. Several other researches have also reported the down-regulation of this miRNA in plasma and tumor tissues of PC patients, according to the literature (22)(23)(24). However, our search for has-miR-125a-3p and Pancreatic cancer in the database of Differentially Expressed MiRNAs in Human Cancers (dbDEMC) (25) revealed that the expression of this miRNA was also up-regulated in two other studies (GSE71533 and GSE74562).…”

Section: Discussionmentioning

confidence: 85%

Experimental Validation of a Potential Circulating microRNA Panel for Non-Invasive Pancreatic Cancer Diagnosis

Salehi¹,

Parsa²,

Roshan-Farzad³

et al. 2021

Preprint

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MicroRNA (miRNA) expression dysregulations in pancreatic ductal adenocarcinoma (PDAC) have been studied widely for their diagnostic and prognostic utility. By the use of Bioinformatics-based methods, in our previous study, we identified some potential miRNA panels for diagnosis of Pancreatic cancer patients from non-cancerous controls (the screening stage). In this report, we used 142 plasma samples from people with and without pancreatic cancer (PC) to conduct RT-qPCR differential expression analysis to assess the strength of the first previously proposed diagnostic panel (consisting of miR-125a-3p, miR-4530 and miR-92a-2-5p). For this aim the research was divided into two phases: testing, and external validation. A total of 92 PC plasma samples and normal controls (NCs) were evaluated in the testing stage to determine the ability of the considered miRNAs to discriminate cancer from non-cancer samples as a panel. Furthermore, in the external validation phase, a group of 25 PC serum samples vs. 25 NCs was employed to validate the diagnostic value of the panel. As the result, we identified significant up-regulation for all the three considered miRNAs in the serum of PC patients. After that, a three-miRNA panel in serum was developed. For the testing, validating and combined stages, the area under the receiver operating characteristic curves (AUC) for the panel were 0.850, 0.910 and 0.86 respectively, indicating that it had a higher diagnostic value than individual miRNAs. Therefore, we detected a promising three-miRNA panel in the plasma for non-invasive PC diagnosis (miR-125a-3p, miR-4530 and miR-92a-2-5p).

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“…It has been reported that circRHOT1 enhances the progression of hepatocellular carcinoma by inducing NR2F6 expression [ 21 ]. CircRHOT1 is involved in the modulation of cell invasion, apoptosis, and proliferation by targeting miR-125a-3p in pancreatic cancer cells [ 11 ]. CircRHOT1 is increased and contributes to cell invasion and proliferation of pancreatic cancer cells [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, Zhang et al suggested that circACC1 facilitated the stabilization and activation of AMP kinase via their interaction, which further modulated glycolysis and fatty acid β-oxidation [ 9 ]. CircRHOT1 is found to be upregulated in several cancers such as pancreatic cancer, hepatocellular carcinoma, and breast cancer [ 10 , 11 ], as well as promotes progression of pancreatic cancer through regulating the proliferation, invasion, and apoptosis, possibly via sponging miR-125a-3p [ 11 , 12 ]. Moreover, circRHOT1 mediates the suppression of propofol on tumorigenesis of NSCLC via affecting miR-326/FOXM1 regulatory axis [ 13 ], which presented circRHOT1 as a possible regulator for NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circRHOT1 contributes to pathogenesis of non-small cell lung cancer by epigenetically enhancing C-MYC expression through recruiting KAT5

Ren

Guo

et al. 2021

Aging

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Non-small cell lung cancer (NSCLC) one of the most prevalent and severe malignancies globally and the molecular mechanisms of NSCLC are poor understood, limiting the development of diagnostic biomarkers and targeted therapies. Circular RNAs (circRNAs) have been identified as a sort of critical regulator in cancer progression. In this study, we identities the epigenetic regulation function of circular RNA circRHOT1 in promoting NSCLC cell proliferation. We found that circRHOT1 were elevated in the clinical tumor tissues relative to that in the peritumor tissues from NSCLC patients. circRHOT1 was up-regulated in human lung cancer cell lines compared with normal human lung epithelial cell line. MTT assays revealed that the silencing of circRHOT1 by siRNA suppressed cell viabilities of NSCLC cells. Colony formation and Edu assays confirmed that circRHOT1 knockdown attenuated NSCLC cell proliferation in vitro . Meanwhile, the depletion of circRHOT1 induced NSCLC cell apoptosis and cell cycle arrest in vitro . Mechanically, the depletion of circRHOT1 remarkably reduced c-MYC mRNA and protein expression in NSCLC cells. Inhibition of circRHOT1 reduced the enrichment of transcription active marker histone H3 lysine 27 acetylation (H3K27ac) and RNA polymerase II on the promoter of c-MYC. RNA pull down analysis showed that circRHOT1 was able to directly interact with acetyltransferase KAT5 in NSCLC cells. In summary, we concluded that circRHOT1 contributed to pathogenesis of NSCLC by epigenetically enhancing c-MYC expression through recruiting KAT5. CircRHOT1 and KAT5 may be used as the potential targets for NSCLC therapy.

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CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‐125a‐3p

Cited by 21 publications

References 26 publications

Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

Experimental Validation of a Potential Circulating microRNA Panel for Non-Invasive Pancreatic Cancer Diagnosis

Circular RNA circRHOT1 contributes to pathogenesis of non-small cell lung cancer by epigenetically enhancing C-MYC expression through recruiting KAT5

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