CircPVT1 promotes gallbladder cancer growth by sponging miR-339-3p and regulates MCL-1 expression

Wang, Shouhua; Su, Ting; Tong, Huanjun; Shi, Wei; Ma, Fei; Quan, Zhiwei

doi:10.1038/s41420-021-00577-y

Cited by 9 publications

(5 citation statements)

References 26 publications

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“…However, some studies have reported that circPVT1 promotes the proliferation of other cancers, including lung cancer, [16][17][18] thyroid cancer, 19,20 and gallbladder cancer. 21 These suggest the dual role of circPVT1 in cancer development, either as a driver or a suppressor. Many miRNAs can be targeted by circPVT1, such as miR-30e, 22 miR-21-5p, 23 miR-429.…”

Section: Discussionmentioning

confidence: 99%

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

Zhou,

Cui,

Zhu

et al. 2024

PGPM

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Background: Circular RNA PVT1 (circPVT1) is aberrantly expressed in several cancers, but its functional role and clinical relevance in bladder urothelial carcinoma (BLCA) remain unknown. This study aimed to identify the expression level of circPVT1 in BLCA and investigated its functional relevance with BLCA progression both in vitro and in vivo. Methods: GEPIA, UALCAN, and OncoLnc were referred to presented data. Quantitative real-time PCR (qPCR) was used for the measurement of transnational expression of genes in BLCA specimens and cell lines. Immunohistochemistry (IHC) and fluorescence in situ hybridization analysis (FISH) assays were performed to detect HER2 amplification, Pearson's correlation analysis to analyze the correlation between circPVT1 expression and clinical characteristics, Cox regression and K-M survival analyses to analyze prognostic factors. A nomogram was constructed for predicting prognosis. The proliferation of cells was measured by CCK-8 and colony formation assay, and the proliferation in vivo was evaluated using nude mouse models. qPCR was used to detect the expression of proliferation-related genes. Results: circPVT1 was but mRNA PVT1 was not significantly overexpressed in BLCA. A high circPVT1 expression was associated with a better survival and negative HER2, but not with age, gender, and T stage. circPVT1 was an independent prognostic factor for the overall survival of BLCA patients. Knocking down circPVT1 promoted BLCA proliferation in vitro and in vivo. Knocking down circPVT1 upregulated ERBB2, MKI67, and PCNA expression and downregulated TP53 expression, but exerted no influence on CCND1 and CCNB1 expression. Conclusion: circPVT1 is a tumor suppressor and novel prognostic biomarker for BLCA.

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Section: Discussionmentioning

confidence: 99%

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

Zhou,

Cui,

Zhu

et al. 2024

PGPM

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“… 24 In osteosarcoma, miR-339-3p is sponged by CircDOCK1 and blunts the sensitivity of osteosarcoma cells to cisplatin. 13 Therefore, miR-339-3p may inhibit cancer tumors. Consistently, we found that the miR-339-3p inhibitor stimulated the migration and proliferation of osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

“… 12 MiR-339-3p has been well documented as a tumor suppressor in various cancers, such as osteosarcoma. 13–15 However, the regulatory mechanisms of circ_0020378 are not well understood.…”

Section: Introductionmentioning

confidence: 99%

hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression

Liu,

Huang

2023

Cancer Biology & Therapy

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Osteosarcoma is a malignant orthopedic tumor that is frequently diagnosed in the pediatric population. Several studies have summarized the functions of circular RNAs (circRNAs) in the progression of osteosarcoma. This study aimed to investigate a novel circRNA, hsa_circ_0020378 (circ_0020378), and elucidate its functions and underlying mechanisms during osteosarcoma progression. The expression levels of circ_0020378, miR-339-3p, and COL1A1 in osteosarcoma cells and tissues were determined using RT-qPCR or Western blotting. CCK8, transwell migration, colony formation, and xenograft experiments were performed to assess the malignancy of osteosarcoma cells. Luciferase and RNA immunoprecipitation (RIP) experiments were employed to validate the interactions of miR-339-3p with circ_0020378 and COL1A1 3′UTR. Osteosarcoma cells and tissues showed significant upregulation of circ_0020378 and COL1A1 and downregulation of miR-339-3p. Silencing circ_0020378 in osteosarcoma cells inhibited their proliferation, colony formation, and migration. The inhibitive influence of circ_0020378 silencing during osteosarcoma tumorigenesis in vitro was verified in vivo. Circ_0020378 sponged miR-339-3p which targeted COL1A1 3′UTR. Circ _0020378 silencing disrupted the tumor-promoting effect of the miR-339-3p inhibitor in osteosarcoma cells. Furthermore, miR-339-3p inhibitor attenuated the suppressive effect of COL1A1 downregulation on malignant osteosarcoma cell phenotypes. Circ_0020378 stimulates osteosarcoma progression by downregulating miR-339-3p/COL1A1 expression. These findings provide a theoretical basis for the discovery of novel osteosarcoma targets.

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“…Circ_PVT1 is involved in the regulation of a variety of cellular events. Circ_PVT1 overexpression increases the invasive capacity of esophageal and gallbladder cancer cells [66,67]. In addition, circ_PVT1 makes lung cancer cells less responsive to Pemetrexed and cisplatin by elevating the expression of ABCC1 (also known as multidrug resistance-associated protein 1, MRP1) [68].…”

Section: Gastric Cancer 231 Circ_0032821 Circ_prrx1 and Circ_pvt1mentioning

confidence: 99%

Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers

Yun

Choi

Son

et al. 2022

IJMS

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Circular RNAs (circRNAs) and long noncoding RNAs (lncRNAs) are differentially expressed in gastrointestinal cancers. These noncoding RNAs (ncRNAs) regulate a variety of cellular activities by physically interacting with microRNAs and proteins and altering their activity. It has also been suggested that exosomes encapsulate circRNAs and lncRNAs in cancer cells. Exosomes are then discharged into the extracellular environment, where they are taken up by other cells. As a result, exosomal ncRNA cargo is critical for cell–cell communication within the cancer microenvironment. Exosomal ncRNAs can regulate a range of events, such as angiogenesis, metastasis, immune evasion, drug resistance, and epithelial-to-mesenchymal transition. To set the groundwork for developing novel therapeutic strategies against gastrointestinal malignancies, a thorough understanding of circRNAs and lncRNAs is required. In this review, we discuss the function and intrinsic features of oncogenic circRNAs and lncRNAs that are enriched within exosomes.

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CircPVT1 promotes gallbladder cancer growth by sponging miR-339-3p and regulates MCL-1 expression

Cited by 9 publications

References 26 publications

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

circPVT1 Inhibits the Proliferation and Aids in Prediction of the Prognosis of Bladder Cancer

hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression

Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers

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