CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

Li, Jun; Ding, Jinli; Qu, Bing; Liu, Jiuying; Song, Xian‐Rong; Suo, Qing-Li; Zhou, Aifen; Yang, Jing

doi:10.1111/jcmm.15747

Cited by 25 publications

(13 citation statements)

References 58 publications

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“…Increasing evidence has demonstrated that PTEN acts as a tumor suppressor functionally involved in many types of cancers, including HCC. 53 , 54 , 55 Interestingly, we verified similar results in this study through survival analysis, IHC, and copy number variation analysis.…”

Section: Discussionsupporting

confidence: 89%

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

Shi

Zhang

Liu

et al. 2021

Molecular Therapy - Nucleic Acids

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Hepatocellular carcinoma (HCC) is one of the deadliest malignant tumors that are harmful to human health. Increasing evidence has underscored the critical role of the competitive endogenous RNA (ceRNA) regulatory networks among various human cancers. However, the complexity and behavior characteristics of the ceRNA network in HCC were still unclear. In this study, we aimed to clarify a phosphatase and tensin homolog (PTEN)-related ceRNA regulatory network and identify potential prognostic markers associated with HCC. The expression profiles of three RNAs (long non-coding RNAs [lncRNAs], microRNAs [miRNAs], and mRNAs) were extracted from The Cancer Genome Atlas (TCGA) database. The DLEU2L-hsa-miR-100-5p/ hsa-miR-99a-5p-TAOK1 ceRNA network related to the prognosis of HCC was obtained by performing bioinformatics analysis. Importantly, we identified the DLEU2L/TAOK1 axis in the ceRNA by using correlation analysis, and it appeared to become a clinical prognostic model by Cox regression analysis. Furthermore, methylation analyses suggested that the abnormal upregulation of the DLEU2L/TAOK1 axis likely resulted from hypomethylation, and immune infiltration analysis showed that the DLEU2L/TAOK1 axis may have an impact on the changes in the tumor immune microenvironment and the development of HCC. In summary, the current study constructing a ceRNA-based DLEU2L/TAOK1 axis might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC.

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Section: Discussionsupporting

confidence: 89%

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

Shi

Zhang

Liu

et al. 2021

Molecular Therapy - Nucleic Acids

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“…It is well known that circRNAs are collectively involved in the initiation and development of PCOS. For example, circPSMC3 suppresses granulosa tumor cell proliferation and facilitates their apoptosis in PCOS [ 18 ]. Additionally, downregulation of circPUM1 enhances proliferation and inhibits apoptosis [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circ_RANBP9 exacerbates polycystic ovary syndrome via microRNA-136-5p/ XIAP axis

et al. 2021

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Polycystic ovary syndrome (PCOS) is an endocrine disease that affects the health of many women. Circular RNAs (circRNAs) are associated with the occurrence and progression of PCOS. This study aimed to explore the function of circ_RANBP9 in PCOS. First, the circ_RANBP9 level was found to be increased in the plasma of patients with PCOS and ovarian granulosa cells (GCs) using Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR). In GCs, loss of circ_RANBP9 decelerated proliferation and accelerated apoptosis of KGN and COV434 cells, as determined by MTT assay, colony formation assay, and flow cytometry. Furthermore, bioinformatics analysis showed that circ_RANBP9 and XIAP can be targeted by the microRNA, miR-136-5p. Luciferase reporter assay and RNA pull-down assay further verified the interaction between miR-136-5p and circ_RANBP9 or XIAP. Importantly, knockdown of circ_RANBP9 suppressed proliferation and promoted apoptosis of KGN and COV434 cells, whereas inhibition of miR-136-5p reversed these effects. Additionally, XIAP abolished the repression of proliferation and acceleration of apoptosis induced by miR-136-5p. The promotion of apoptosis was accompanied by upregulation of caspase-3 and Bax, and downregulation of Bcl-2, as estimated by western blotting. In conclusion, silencing of circ_RANBP9 inhibited GC proliferation and facilitated apoptosis by mediating the miR-136-5p/XIAP pathway. These findings provide a new theoretical basis for screening and treatment of PCOS.

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“…circRNAs collectively participate in the initiation and development of endocrine dyscrasia in females. For instance, dysregulated circRNAs are associated with the progression of gestational diabetes mellitus, cardiovascular diseases, obesity, and PCOS [9][10][11][12]. Overexpressed circRNA_0054633 contributes to gestational diabetes mellitus and the increase of glycosylation index [27].…”

Section: Discussionmentioning

confidence: 99%

“…This confer its stability in various biological functions, such as post-transcription, RNA-related protein binding, gene splicing, and modi cation of parental gene expression [7,8]. However, dysfunction of circRNAs is evidenced to be associated with endocrinopathy, such as type 2 diabetes mellitus, cardiovascular diseases, obesity, and PCOS [9][10][11][12]. For instance, knockdown of circRNA_DMNT3B contributes to diabetic nephropathy via regulating miR-20b-5p/BAMBI [13].…”

Section: Introductionmentioning

confidence: 99%

A Novel ACTL6A Exonic Circular RNA Exacerbates Polycystic Ovary Syndrome via Recruiting TET1

Zhang¹,

Wang²,

Li³

et al. 2021

Preprint

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Polycystic ovary syndrome (PCOS) is an endocrine disorder prevailing among females at reproductive age. Circular non-coding RNAs (circRNAs) are essential “player” in PCOS. This study aimed to investigate the potentials of newly found circRNA, circ_ACTL6A in PCOS. Dehydroepiandrosterone (DHEA) was utilized to establish PCOS model in vivo. qRT-PCR and western blot were applied to determine the gene expression level. DNA methylation was determined by bisulfite sequencing assay. RNA pull-down, Chromatin immunoprecipitation (ChIP) assay, and RNA immunoprecipitation (RIP) assay was applied to verify gene interactions. CCK-8, colony formation and 5-Ethynyl-2'-deoxyuridine (EdU) assay were applied to determine cellular function of human granulosa-like tumor cell line (KGN) cells. The results showed that circ_ACTL6A was upregulated in PCOS, which was due to the upregulation of RNA splicing factor eukaryotic translation initiation factor 4A3 (EIF4A3) and its cognate protein actin like 6A (ACTL6A). Downregulation of circ_ACTL6A suppressed the cell viability, colony formation and proliferation of KGN cells in vitro. Moreover, knockdown of circ_ACTL6A suppressed the progression of PCOS, manifested by the improvement in insulin sensitivity, ovarian function and fertility. Furthermore, circ_ACTL6A recruited tet methylcytosine dioxygenase 1 (TET1) to suppress DNA methylation of ACTL6A. Overexpression of ACTL6A promoted the development of PCOS in vivo and in vitro. Taken together, circ_ACTL6A/ACTL6A may form a positive feedback in the progression of PCOS. This may be a potential therapeutic target for PCOS.

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CircPSMC3 alleviates the symptoms of PCOS by sponging miR‐296‐3p and regulating PTEN expression

Cited by 25 publications

References 58 publications

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

Comprehensive analysis to identify DLEU2L/TAOK1 axis as a prognostic biomarker in hepatocellular carcinoma

Circular RNA circ_RANBP9 exacerbates polycystic ovary syndrome via microRNA-136-5p/ XIAP axis

A Novel ACTL6A Exonic Circular RNA Exacerbates Polycystic Ovary Syndrome via Recruiting TET1

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