CircPRKCI relieves lipopolysaccharide‐induced HK2 cell injury by upregulating the expression of miR‐545 target gene ZEB2

Shi, Xiaofeng; Ma, Wei; Li, Yongqi; Wang, Han; Pan, Shuang; Pan, Yongquan; Xu, Caiming; Li, Lei

doi:10.1002/biof.1620

Cited by 26 publications

(13 citation statements)

References 37 publications

(64 reference statements)

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“…In Hu's study of the mammary glands,he showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187. The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer [41]. In Hu's study of the mammary glands he showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187.…”

Section: Discussionmentioning

confidence: 95%

WITHDRAWN: High-throughput Analysis of CircRNA in Cows with Naturally Infected Staphylococcus aureus Mammary Gland

Bai

Cai

Zhang

et al. 2021

Preprint

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Circular RNAs (CircRNA) is a special type of non-coding RNA molecule with a closed ring structure and is not affected by RNA exonucases. It has stable expression and is not easy to degrade, and exists in most eukaryotes. However, circRNA regulation of cow mastitis has not been widely recognized. Mammary epithelial tissues were collected from healthy Holstein cows (HCN) and mastitis Holstein cows (HCU). RNA sequencing (RNA SEQ) was performed for the differentially expressed circRNAs, and analysis results showed that 19 differentially expressed circRNAs were identified in HCN and HCU, among which 6 circRNAs were up-regulated and 13 circRNAs were down-regulated. We randomly selected 9 circRNAs for Q-PCR verification, and the results showed consistent expression. Three circRNAs: circRNA2860, circRNA5323 and circRNA4027 were confirmed to be significantly differentially expressed circRNAs in cow mastitis. Furthermore, RNA polymerase transcription factor binding and tight junction are most enriched in GO and KEGG pathways, respectively. In addition, the regulatory network of circRNA-miRNA has been inferred from a bioinformatics perspective, which may help to understand the underlying molecular mechanism of circRNAs involved in regulating mastitis in cows.

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Section: Discussionmentioning

confidence: 95%

WITHDRAWN: High-throughput Analysis of CircRNA in Cows with Naturally Infected Staphylococcus aureus Mammary Gland

Bai

Cai

Zhang

et al. 2021

Preprint

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“…A variety of endotoxins including LPS have been proved to stimulate the immune network response of the body to release inflammatory factors, and then lead to functional injury of kidney and other organs 24,25 . In addition, LPS‐induced HK2 cell injury has become a common in vitro cell model of septic AKI, providing convenience for performing the research on sepsis‐induced AKI 26 . Here, we investigated the circ‐ZNF644 effects on LPS‐treated HK2 cell injury to confirm its potential function in sepsis‐induced AKI.…”

Section: Discussionmentioning

confidence: 99%

“…24,25 In addition, LPS-induced HK2 cell injury has become a common in vitro cell model of septic AKI, providing convenience for performing the research on sepsis-induced AKI. 26 Here, we investigated the circ-ZNF644 effects on LPS-treated HK2 cell injury to confirm its potential function in sepsis-induced AKI. Our data indicated that circ-ZNF644 had an increased expression in sepsisinduced AKI patients.…”

Section: Mir-335-5p Inhibited Lps-induced Hk2 Cell Injury By Hipk1mentioning

confidence: 99%

Downregulation of circ‐ZNF644 alleviates LPS‐induced HK2 cell injury via miR‐335‐5p/HIPK1 axis

Gong

Zhao

Luo

et al. 2022

Environmental Toxicology

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Circular RNA (circRNA) has been confirmed to be involved in regulating sepsis‐induced acute kidney injury (AKI). Our research aims to explore circ‐ZNF644 role in the development of sepsis‐induced AKI. Lipopolysaccharide (LPS) was used to induce kidney tubular epithelial cell (HK2) injury. ELISA assay was performed to measure the concentrations of inflammation factors. Cell functions were determined by cell counting kit 8 assay, EdU assay and flow cytometry. Protein expression was evaluated by Western blot analysis. Quantitative real‐time PCR was used to detect relative expression of circ‐ZNF644, miR‐335‐5p and homeodomain‐interacting protein kinase 1 (HIPK1). RNA interaction was confirmed by dual‐luciferase reporter assay and RIP assay. LPS enhanced HK2 cell inflammation, oxidative stress, apoptosis, and reduced proliferation. Circ‐ZNF644 was overexpressed in sepsis‐induced AKI patients. Circ‐ZNF644 knockdown suppressed LPS‐induced HK2 cell injury, and this effect could be revoked by miR‐335‐5p inhibitor. MiR‐335‐5p was sponged by circ‐ZNF644, and its expression was downregulated in sepsis‐induced AKI patients. HIPK1 was targeted by miR‐335‐5p, and its expression could be suppressed by circ‐ZNF644 knockdown. MiR‐335‐5p had an inhibition effect on HK2 cell injury induced by LPS, and HIPK1 overexpression could reverse this effect. Circ‐ZNF644 knockdown relieved LPS‐induced HK2 cell injury through the miR‐335‐5p/HIPK1 axis, confirming that circ‐ZNF644 contributed to sepsis‐induced AKI.

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“…Meanwhile, it has been found that circPRKCI is a ceRNA to enhance AKT3 expression in esophageal squamous cell carcinoma via sponging miR-3680-3p [ 19 ]. CircPRKCI attenuates lipopolysaccharide-stimulated injury of HK2 cells by the up-regulation of ZEB2 by sponging miR-545 [ 20 ]. CircPRKCI depletion contributes to radiosensitivity and represses progression of esophageal cancer by the regulation of miR-186-5p/PARP9 axis [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circPRKCI contributes to malignant progression of T-cell acute lymphoblastic leukemia by modulating miR-20a-5p/SOX4 axis

Zheng

Niu

Zhang

et al. 2021

Aging

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Circular RNAs (circRNAs) have demonstrated critical roles in the development of cancers. This study aimed to explore the function of circular RNA circPRKCI/miR-20a-5p/SOX4 axis in acute lymphoblastic leukemia (ALL). Our data showed that the expression of circPRKCI and SOX4 was enhanced while the expression of miR-20a-5p was reduced in the clinical T-ALL samples. The expression of miR-20a-5p was negatively associated with circPRKCI and SOX4 in the T-ALL patients and the expression of circPRKCI was positive correlated with SOX4 in the T-ALL patients. Functionally, the silencing of circPRKCI suppressed the viability of T-ALL cells. Conversely, the knockdown of circPRKCI promoted the apoptosis of T-ALL cells. The levels of cleaved PARP and cleaved caspase3 were induced by the depletion of circPRKCI in T-ALL cells. Mechanically, the luciferase activity of circPRKCI was significantly decreased in T-ALL cells after the treatment of miR-20a-5p mimic. Meanwhile, the silencing of circPRKCI promoted the expression of miR-20a-5p in T-ALL cells, implying that circPRKCI serves as a competitive endogenous RNAs (ceRNA) of miR-20a-5p. We validated that the treatment of miR-20a-5p mimic inhibited the viability of T-ALL cells. MiR-20a-5p mimic enhanced the apoptosis of T-ALL cells. The expression of cleaved PARP and cleaved caspase3 was increased by miR-20a-5p mimic in the cells. In summarization, we concluded that circular RNA circPRKCI contributed to malignant progression of T-cell acute lymphoblastic leukemia by modulating miR-20a-5p/SOX4 axis. Targeting circPRKCI may serve as a promising therapeutic strategy of T-ALL.

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CircPRKCI relieves lipopolysaccharide‐induced HK2 cell injury by upregulating the expression of miR‐545 target gene ZEB2

Cited by 26 publications

References 37 publications

WITHDRAWN: High-throughput Analysis of CircRNA in Cows with Naturally Infected Staphylococcus aureus Mammary Gland

WITHDRAWN: High-throughput Analysis of CircRNA in Cows with Naturally Infected Staphylococcus aureus Mammary Gland

Downregulation of circ‐ZNF644 alleviates LPS‐induced HK2 cell injury via miR‐335‐5p/HIPK1 axis

Circular RNA circPRKCI contributes to malignant progression of T-cell acute lymphoblastic leukemia by modulating miR-20a-5p/SOX4 axis

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