CircPRKCI-miR-545/589-E2F7 axis dysregulation mediates hydrogen peroxide-induced neuronal cell injury

Cheng, Qingqing; Cao, Xiangyang; Xue, Liujun; Xia, Lei; Xu, Yun

doi:10.1016/j.bbrc.2019.04.131

Cited by 20 publications

(17 citation statements)

References 25 publications

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“…Two primary miR-545 targets are RIG-1 and E2F7 in cancer cells 16,26,28,34 . RIG-I is an intracellular viral RNA sensor, whose activation could initiate host innate immune response to increase type I IFN production 35 .…”

Section: Discussionmentioning

confidence: 99%

“…The full-length circPRKCI 16,26 and pri-miR-545 16,26 were synthesized by Shanghai Genechem Co, sub-cloned to a lentiviral GV248 construct (Shanghai Genechem Co.). The construct, together with lentivirus package plasmid mix, was co-transfected to HEK-293 cells to generate circPRKCI-expressing lentivirus (“LV-circPRKCI”) and miR-545-expressing lentivirus (“LV-miR-545”).…”

Section: Methodsmentioning

confidence: 99%

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Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545

Zhang

Han

et al. 2019

Cell Death Dis

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We here tested expression and potential functions of circular RNA PRKCI (circPRKCI) in human glioma. Our results show that circPRKCI is upregulated in human glioma tissues and glioma cells, correlating with downregulation of its potential target, microRNA-545 (miR-545). In A172 and primary human glioma cells, shRNA-mediated silencing of circPRKCI inhibited cancer cell growth, survival, proliferation, and migration. Conversely, ectopic circPRKCI overexpression promoted A172 cell progression. miR-545 is the primary target of circPRKCI in glioma cells. Forced overexpression of miR-545 mimicked circPRKCI shRNA-induced actions, inhibiting glioma cell survival and proliferation. In contrast, miR-545 inhibition, by a lentiviral antagomiR-545 construct, reversed circPRKCI shRNA-induced anti-A172 cell activity. Importantly, neither circPRKCI shRNA nor circPRKCI overexpression was effective in miR-545-knockout (Cas9 method) A172 cells. Importantly, the subcutaneous and orthotopic A172 xenograft growth was significantly inhibited by circPRKCI silencing. Collectively, circPRKCI promotes human glioma cell progression possibly by inhibiting miR-545. Targeting circPRKCI-miR-545 cascade could efficiently inhibit human glioma cells.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545

Zhang

Han

et al. 2019

Cell Death Dis

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“…Cheng et al show that H 2 O 2 downregulates the expression of circPRKCI (protein kinase C iota) and induces neuronal injury through the circPRKCI-miR-545/589-E2F2 axis. The overexpression of circPRKCI alleviates H 2 O 2 -induced cytotoxicity [92]. There are 15 circRNAs significantly changed in an oxygen-glucose deprivation/reoxygenation (OGD/R) model of cultured hippocampal HT22 cells, and the upregulated expression of mmu-circRNA-015947 indicated that it may be involved in the pathogenesis of cerebral IRI through the Kyoto Encyclopedia of Genes and Genomes pathway analysis [62].…”

Section: Circrnas Regulate Other Neurological Diseases Viamentioning

confidence: 99%

Emerging Clues of Regulatory Roles of Circular RNAs through Modulating Oxidative Stress: Focus on Neurological and Vascular Diseases

Jiang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Circular RNAs (circRNAs) are novel noncoding RNAs that play regulatory roles in gene expression. Dysregulation of circRNAs is associated with the development and progression of several diseases, such as diabetes mellitus, nervous system diseases, cardiovascular diseases, and cancer. CircRNAs functionally participate in cell physiological activities through various molecular mechanisms. However, these molecular mechanisms are unclear. Oxidative stress is an essential factor in the pathogenesis of various diseases, including neurological diseases. Emerging roles of circRNAs have been identified in different systems in response to oxidative stress. In this review, we summarize the current understanding of circRNA biogenesis, properties, expression profiles, and the clues indicating the regulatory roles of circRNAs through oxidative stress in various systems, especially the nervous system.

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“…They mediate the production of ROS and promote ROS-induced cell death, apoptosis, and inflammation (Saaoud et al, 2021). Studies have shown that L02 cells exhibit substantially increased circRNA-4099 levels in response to hydrogen peroxide (H 2 O 2 )-induced oxidative stress, and the generated circRNA-4099 further triggers the activation of the keap1/Nrf2-p38 MAPK cascade by enhancing the phosphorylation of related proteins, downregulating miR-706 expression, and enhancing the intracellular H 2 O 2 levels, thereby promoting apoptosis, ROS generation, and fibrosis (Cheng et al, 2019).…”

Section: Circrna Regulates Reactive Oxygen Species Productionmentioning

confidence: 99%

Circular RNAs in the Regulation of Oxidative Stress

et al. 2021

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Oxidative stress caused by an imbalance between the production and elimination of reactive metabolites and free radicals can lead to the development of a variety of diseases. Over the past years, with the development of science and technology, circular RNA (circRNA) has been found to be closely associated with oxidative stress, which plays an important role in the process of oxidative stress. Currently, the understanding of circRNAs in the mechanism of oxidative stress is limited. In this review, we described the relationship between oxidative stress and circRNAs, the circRNAs related to oxidative stress, and the role of circRNAs in promoting or inhibiting the occurrence and development of diseases associated with the oxidative stress system.

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CircPRKCI-miR-545/589-E2F7 axis dysregulation mediates hydrogen peroxide-induced neuronal cell injury

Cited by 20 publications

References 25 publications

Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545

Circular RNA PRKCI promotes glioma cell progression by inhibiting microRNA-545

Emerging Clues of Regulatory Roles of Circular RNAs through Modulating Oxidative Stress: Focus on Neurological and Vascular Diseases

Circular RNAs in the Regulation of Oxidative Stress

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