CircPLXNB2 regulates acute myeloid leukemia progression through miR-654-3p/
            <i>CCND1</i>
            axis

Wang, Jiao; Wu, Chunyan; Zhou, Weilun

doi:10.1080/16078454.2023.2220522

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…Several studies have shown that overexpression of CBX7 and loss of H3K9me3 are related to the emergence of AML [19][20][21] , so we analyzed the transcriptome of mCherry-CBX7 3T3 cell lines. We detected up-regulation of leukemia-related genes [22][23][24][25] , and down-regulation of genes related to nucleosome assembly and genomic stability (Extended Data Fig. 6a-c).…”

mentioning

confidence: 95%

Histone marks enable formation of immiscible phase-separated chromatin compartments

Sun,

Wang,

Liu

et al. 2023

Preprint

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Eukaryotic chromatin is organized into compartments for gene expression regulation, but the underlying mechanisms remain unclear. Here, we demonstrate that multivalent H3K27me3 and its reader, CBX7-PRC1 complex, regulate facultative heterochromatin via a phase separation mechanism, similar to constitutive heterochromatin1. Facultative and constitutive heterochromatin represent distinct, coexisting condensates in nuclei.In vitro,H3K27me3- and H3K9me3-marked nucleosomal arrays and their reader complexes can phase separate into immiscible condensates that are analogous to the relationship between facultative and constitutive heterochromatinin vivo. Moreover, overexpression of CBX7-PRC1 causes aberrant chromatin compartmentalizationin vivoas demonstrated by H3K9me3 CUT&Tag and up-regulation of genes related to cancer such as acute myeloblastic leukemia (AML). CBX7 inhibitor effectively inhibits cancer cell proliferation possibly through phase separation-mediated compartment reorganization. Our data demonstrated how the specificity of compartmentalization is achieved based on the formation of immiscible phase-separated condensates, and offer novel epigenetic mechanistic insights into tumor development.

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confidence: 95%

Histone marks enable formation of immiscible phase-separated chromatin compartments

Sun,

Wang,

Liu

et al. 2023

Preprint

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On the mechanism of wogonin against acute monocytic leukemia using network pharmacology and experimental validation

Wang,

Chen

et al. 2024

Sci Rep

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Wogonin is a natural flavone compound from the plant Scutellaria baicalensis, which has a variety of pharmacological activities such as anti-cancer, anti-virus, anti-inflammatory, and immune regulation. However, the potential mechanism of wogonin remains unknown. This study was to confirm the molecular mechanism of wogonin for acute monocytic leukemia treatment, known as AML-M5. The potential action targets between wogonin and acute monocytic leukemia were predicted from databases. The compound-target-pathway network and protein-protein interaction network (PPI) were constructed. The enrichment analysis of related targets and molecular docking were performed. The network pharmacological results of wogonin for AML-M5 treatment were verified using the THP-1 cell line. 71 target genes of wogonin associated with AML-M5 were found. The key genes TP53, SRC, AKT1, RELA, HSP90AA1, JUN, PIK3R1, and CCND1 were preliminarily found to be the potential central targets of wogonin for AML-M5 treatment. The PPI network analysis, GO analysis and KEGG pathway enrichment analysis demonstrated that the PI3K/AKT signaling pathway was the significant pathway in the wogonin for AML-M5 treatment. The antiproliferative effects of wogonin on THP-1 cells of AML-M5 presented a dose-dependent and time-dependent manner, inducing apoptosis, blocking the cell cycle at the G2/M phase, decreasing the expressions of CCND1, CDK2, and CyclinA2 mRNA, as well as AKT and p-AKT proteins. The mechanisms of wogonin on AML-M5 treatment may be associated with inhibiting cell proliferation and regulating the cell cycle via the PI3K/AKT signaling pathway.

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CircPLXNB2 regulates acute myeloid leukemia progression through miR-654-3p/ CCND1 axis

Cited by 2 publications

References 29 publications

Histone marks enable formation of immiscible phase-separated chromatin compartments

Histone marks enable formation of immiscible phase-separated chromatin compartments

On the mechanism of wogonin against acute monocytic leukemia using network pharmacology and experimental validation

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