CircNUP98 promotes the malignant behavior of glioma cells through the miR‐520f‐3p/ELK4 axis

Abstract

Glioma, a formidable form of brain cancer, poses significant challenges in terms of treatment and prognosis. Circular RNA nucleoporin 98 (circNUP98) has emerged as a potential regulator in various cancers, yet its role in glioma remains unclear. Here, we elucidate the functional role of circNUP98 in glioma cell proliferation, invasion, and migration, shedding light on its therapeutic implications. Glioma cells were subjected to si‐NUP98 transfection, followed by assessments of cell viability, proliferation, invasion, and migration. Subcellular localization of circNUP98 was determined, and its downstream targets were identified. We delineated the binding relationships between circNUP98 and microRNA (miR)‐520f‐3p, as well as between miR‐520f‐3p and ETS transcription factor ELK4 (ELK4). The expression levels of circNUP98/miR‐520f‐3p/ELK4 were quantified. Our findings demonstrated that circNUP98 was upregulated in glioma cells, and its inhibition significantly attenuated glioma cell proliferation, invasion, and migration. Mechanistically, circNUP98 functioned as a sponge for miR‐520f‐3p, thereby relieving the inhibitory effect of miR‐520f‐3p on ELK4. Moreover, inhibition of miR‐520f‐3p or overexpression of ELK4 partially rescued the suppressive effect of circNUP98 knockdown on glioma cell behaviors. In summary, our study unveils that circNUP98 promotes glioma cell progression via the miR‐520f‐3p/ELK4 axis, offering novel insights into the therapeutic targeting of circNUP98 in glioma treatment.