CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway

Xie, Ying; Hang, Xiaofeng; Xu, Wenrong; Gu, Jiang; Zhang, Yuanjing; Wang, Jianrong; Zhang, Xiucui; Cao, Xinghao; Zhan, Junjie; Wang, Junxue; Gan, Jing

doi:10.1186/s12935-021-02120-6

Cited by 14 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that circRNAs can act as sponges of miRNAs to modulate gene expression in cancer. For example, circFAM13B can sponge miR-212 to promote the proliferation of hepatocellular carcinoma ( Xie et al, 2021 ). CircCUL2 regulates gastric cancer malignant transformation by sponging miR-142-3p ( Peng et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA CDR1as Alleviates Cisplatin-Based Chemoresistance by Suppressing MiR-1299 in Ovarian Cancer

Zhao

Wang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Cisplatin (CDDP) chemoresistance seriously affects the prognosis and survival of patients with ovarian cancer (OC). Previous research has shown that circular RNA CDR1as is biologically associated with a large number of cancers. However, the molecular mechanism underlying the role of CDR1as in CDDP chemoresistance in OC remains unclear. Here, we investigated the mechanism of CDR1as in CDDP-resistant OC. First, we employed bioinformatics analysis and quantitative real-time PCR (qRT-PCR) to determine the expression of CDR1as and related RNAs in CDDP-sensitive and -resistant OC tissues and cells. Then, functional experiments were used to determine cell proliferation, invasion, migration, and apoptosis in CDDP chemoresistance and parent OC cells in vitro. The effect of CDR1as in CDDP chemoresistance OC progression was tested in nude mice in vivo. Moreover, dual-luciferase assays and RNA immunoprecipitation (RIP) were performed to confirm the interactions of CDR1as and related RNAs. Finally, we used Western blotting to determine protein expression levels. Our findings interpret the underlying mechanisms of the CDR1as/miR-1299/PPP1R12B axis and shed light on the clinical applications for CDDP-chemoresistant OC.

show abstract

Section: Discussionmentioning

confidence: 99%

Circular RNA CDR1as Alleviates Cisplatin-Based Chemoresistance by Suppressing MiR-1299 in Ovarian Cancer

Zhao

Wang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Circ_0014717 may be a promising biomarker for HCC through the miR‐668‐3p/BTG2 pathway 21 . CircFAM13B promotes the progression of HCC via the miR‐212/E2F5 axis and activates the P53 axis 22 . Has_circ_0016788 regulates the proliferation and glycolysis of HCC by sponging miR‐506‐3p and upregulating PARP14 23…”

Section: Discussionmentioning

confidence: 99%

“…21 CircFAM13B promotes the progression of HCC via the miR-212/E2F5 axis and activates the P53 axis. 22 Has_circ_0016788 regulates the proliferation and glycolysis of HCC by sponging miR-506-3p and upregulating PARP14. 23 In this study, we found that circ_0001459 was significantly enhanced in HCC tissue and cells compared with normal control tissue.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circ_0001459 accelerates hepatocellular carcinoma progression via the miR‐6165/IGF1R axis

Shen

Zhao

Zeng

et al. 2022

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

An increasing amount of evidence shows that circular RNAs (circRNAs) have critical effects on cancer progression and development; however, the biological function and potential molecular mechanism of circRNAs in hepatocellular carcinoma (HCC) are still unclear. CircRNA sequencing was used to identify differentially expressed circRNAs between HCC tissue and adjacent normal tissue. We found that circ_0001459 expression was significantly elevated in HCC tissue and cell lines. Furthermore, in vitro and in vivo functional experiments were carried out to detect the effects of circ_0001459 on HCC growth and metastasis. Knockdown of circ_0001459 significantly inhibited the proliferation, migration, and invasion of HCC cells, whereas upregulation of circ_0001459 had the opposite effect. Moreover, bioinformatics analysis, dual-luciferase reporter assay, RNA immunoprecipitation, and fluorescence in situ hybridization assays were used to predict and verify the interaction between circ_0001459, miR-6165, and the target gene IGF1R. Downregulation of circ_0001459 decreased IGF1R expression and inhibited epithelialto-mesenchymal transition, which could be rescued by treatment with a miR-6165 inhibitor. Mechanistically, we revealed that circ_0001459 could sponge miR-6165 and induce the upregulation of its downstream target IGF1R, thus significantly promoting the progression of HCC. Therefore, circ_0001459 could be a new potential therapeutic target for HCC patients.

show abstract

“…Meanwhile, as their cyclization products circFAM13B and circCORO1C are also associated with the development of PD. CircFAM13B can activate the P53 pathway by sponging miR-212 [57]. P53 is a key tumor suppressor that controls a number of cellular processes, including growth arrest and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Circular RNAs in Parkinson’s Disease: Reliable Biological Markers and Targets for Rehabilitation

et al. 2023

View full text Add to dashboard Cite

In clinical practice, the underlying pathogenesis of Parkinson's disease (PD) remains unknown. Circular RNAs (circRNAs) have good biological properties and can be used as biological marker. Rehabilitation as a third treatment alongside drug and surgical has been shown to be clinically effective, but biomarkers of rehabilitation e ciency at genetic level is still lacking. In this study we identi ed differentially expressed circRNAs in peripheral blood exosomes between PD patients and health controls (HCs) and determined whether these circRNAs changed after rehabilitation, to explore the competing RNA networks and epigenetic mechanisms affected. We found that there were 558 upregulated and 609 downregulated circRNAs in PD patients compared to HCs, 3398 upregulated and 479 downregulated circRNAs in PD patients after rehabilitation compared to them before rehabilitation, along with 3721 upregulated and 635 downregulated circRNAs in PD patients after rehabilitation compared to HCs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that differentially expressed circRNAs may affect the stability of the cellular actin backbone and synaptic structure by in uencing the aggregation of α-Synuclein (a-syn). We selected two circRNAs overexpressed in PD patients for validation (hsa_circ_0001535 and hsa_circ_0000437), the results revealed that their expression levels were all reduced to varying degrees (p < 0.05) after rehabilitation. After network analysis, we believe that hsa_circ_0001535 may be related to the aggregation of a-syn, while hsa_circ_0000437 may act on hsa-let-7b-5p or hsa-let-7c-5p through sponge effect to cause in ammatory response. Our ndings suggest that rehabilitation can mitigate the pathological process of PD by epigenetic means.

show abstract

CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway

Cited by 14 publications

References 20 publications

Circular RNA CDR1as Alleviates Cisplatin-Based Chemoresistance by Suppressing MiR-1299 in Ovarian Cancer

Circular RNA CDR1as Alleviates Cisplatin-Based Chemoresistance by Suppressing MiR-1299 in Ovarian Cancer

Circular RNA circ_0001459 accelerates hepatocellular carcinoma progression via the miR‐6165/IGF1R axis

Circular RNAs in Parkinson’s Disease: Reliable Biological Markers and Targets for Rehabilitation

Contact Info

Product

Resources

About