Circadian Rhythms of Locomotor Activity in Zebrafish

Hurd, Mark W.; DeBruyne, Jason P.; Straume, Martin; Cahill, Gregory M.

doi:10.1016/s0031-9384(98)00183-8

Cited by 147 publications

(92 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Clock-controlled Gene in per2 Mutant Zebrafish-Locomotor activities of zebrafish larvae exhibit robust circadian rhythmicity and peak during the subjective day (57). To determine whether Per2 affects locomotor activity rhythms, behavior analyses were performed for per2 mutant and wild-type larvae starting at 96 h postfertilization under LD and DD conditions.…”

Section: Disrupted Rhythmicity Of Locomotor Activities and Altered Exmentioning

confidence: 99%

The Zebrafish Period2 Protein Positively Regulates the Circadian Clock through Mediation of Retinoic Acid Receptor (RAR)-related Orphan Receptor α (Rorα)

Wang

Zhong

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Null mutants for zebrafish period2 were generated to elucidate its functions. Results: Both locomotor activity and expression of key circadian clock genes are disrupted in the period2 mutant zebrafish. Conclusion: Period2 is essential for zebrafish circadian regulation. Significance: Period2 plays a positive role in the zebrafish circadian clock by enhancing bmal1b expression through binding to nuclear receptor Ror␣.

show abstract

Section: Disrupted Rhythmicity Of Locomotor Activities and Altered Exmentioning

confidence: 99%

The Zebrafish Period2 Protein Positively Regulates the Circadian Clock through Mediation of Retinoic Acid Receptor (RAR)-related Orphan Receptor α (Rorα)

Wang

Zhong

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…In rats it has been shown that UCS has a stronger impact on physiology and behaviour when given during the resting phase than during the active (or awake) phase (Aslani et al, 2014). Zebrafish are active during the daytime and rest during the night-time (Hurd et al, 1998). Disturbances during the night-time may negatively impact zebrafish behaviour (Löhr and Hammerschmidt, 2011;Singh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Manuel

Gorissen

Zethof

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Zebrafish (Danio rerio Hamilton) are increasingly used as a model to study the effects of chronic stress on brain and behaviour. In rodents, unpredictable chronic stress (UCS) has a stronger effect on physiology and behaviour during the active phase than during the resting phase. Here, we applied UCS during the daytime (active phase) for 7 and 14 days or during the night-time (resting phase) for 7 nights in an in-house-reared Tuebingen long-fin (TLF) zebrafish strain. Following UCS, inhibitory avoidance learning was assessed using a 3 day protocol where fish learn to avoid swimming from a white to a black compartment where they will receive a 3 V shock. Latencies of entering the black compartment were recorded before training (day 1; first shock) and after training on day 2 (second shock) and day 3 (no shock, tissue sampling). Fish whole-body cortisol content and expression levels of genes related to stress, fear and anxiety in the telencephalon were quantified. Following 14 days of UCS during the day, inhibitory avoidance learning decreased (lower latencies on days 2 and 3); minor effects were found following 7 days of UCS. Following 7 nights of UCS, inhibitory avoidance learning decreased (lower latency on day 3). Whole-body cortisol levels showed a steady increase compared with controls (100%) from 7 days of UCS (139%), to 14 days of UCS (174%) to 7 nights of UCS (231%), suggestive of an increasing stress load. Only in the 7 nights of UCS group did expression levels of corticoid receptor genes (mr, grα, grβ) and of bdnf increase. These changes are discussed as adaptive mechanisms to maintain neuronal integrity and prevent overload, and as being indicative of a state of high stress load. Overall, our data suggest that stressors during the resting phase have a stronger impact than during the active phase. Our data warrant further studies on the effect of UCS on stress axis-related genes, especially grβ; in mammals this receptor has been implicated in glucocorticoid resistance and depression.

show abstract

“…The circadian period of melatonin production for each pineal was determined from a cosine fit to the raw integer data by a Fast Fourier Transform coupled to a nonlinear least squares routine (FFT-NLLS; Hurd et al, 1998;Plautz et al, 1997). One-way and two-way analyses of variance (ANOVA), as well as post-hoc comparisons, were performed using Statistica '98 software (StatSoft Inc., Tulsa, OK).…”

Section: The Lag Rhythm Mutant In Zebrafishmentioning

confidence: 99%

“…Zebrafish exhibit circadian rhythms of behavior as larvae and as adults, Delaunay et al, 2000;Hurd et al, 1998;Kazimi & Cahill, 1999) with peaks of behavioral activity during the daytime. Oscillators within the brain presumably drive these rhythms since pinealectomy and ocular enucleation do not affect behavioral rhythmicity of larvae (M.W.H.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Phenogenetics of a Novel Circadian Rhythm Mutant in Zebrafish

DeBruyne

Hurd

Gutierrez

et al. 2004

Journal of Neurogenetics

View full text Add to dashboard Cite

Widespread use of zebrafish (Danio rerio) in genetic analysis of embryonic development has led to rapid advances in the technology required to generate, map and clone mutated genes. To identify genes involved in the generation and regulation of vertebrate circadian rhythmicity, we screened for dominant mutations that affect the circadian periodicity of larval zebrafish locomotor behavior. In a screen of 6,500 genomes, we recovered 8 homozygous viable, semi-dominant mutants, and describe one of them here. The circadian period of the lager and lime (lag dg2 ) mutant is shortened by 0.7 h in heterozygotes, 403 and 1.3 h in homozygotes. This mutation also shortens the period of the melatonin production rhythm measured from cultured pineal glands, indicating that the mutant gene product affects circadian rhythmicity at the tissue level, as well as at the behavioral level. This mutation also alters the sensitivity of pineal circadian period to temperature, but does not affect phase shifting responses to light. Linkage mapping with microsatellite markers indicates that the lag mutation is on chromosome 7. A zebrafish homolog of period1 ðper1Þ is the only known clock gene homolog that maps near the lag locus. However, all sequence variants found in per1 cDNA from lag dg2 mutants are also present in wild type lines, and we were unable to detect any defect in per1 mRNA splicing, so this mutation may identify a novel clock gene.

show abstract

Circadian Rhythms of Locomotor Activity in Zebrafish

Cited by 147 publications

References 42 publications

The Zebrafish Period2 Protein Positively Regulates the Circadian Clock through Mediation of Retinoic Acid Receptor (RAR)-related Orphan Receptor α (Rorα)

The Zebrafish Period2 Protein Positively Regulates the Circadian Clock through Mediation of Retinoic Acid Receptor (RAR)-related Orphan Receptor α (Rorα)

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Isolation and Phenogenetics of a Novel Circadian Rhythm Mutant in Zebrafish

Contact Info

Product

Resources

About