Circadian Rhythms of Intestinal Disaccharidases in Experimental Uremia in Rats

Inagaki, Oshi; Mori, Hideki; Shimomura, Rinpei; Inoue, Seishi; Fujita, Yoshikazu

doi:10.1159/000182514

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Circadian rhythmicity has also been observed for the activity of various small-intestine functions, including the activity of disaccharidases such as sucrase-isomaltase, maltase, lactase and trehalase [29,30,40,41], the intestinal absorption of L-histidine [39], the activity of y-glutamyltransferase and alkaline phosphatase and leucylnaphthylamide-hydrolysing activity [40]. The abundance of sucrase-isomaltase mRNA shows a similar circadian rhythm to that found for GLUT5 mRNA levels and, curiously enough, the two transcripts also share a similar distribution along the crypt-villus axis.…”

Section: Discussionmentioning

confidence: 97%

“…It has been reported that proteins expressed in intestine, such as disaccharidases, show circadian rhythmic changes [29,30]. In order to determine whether GLUT5 gene expression is also subjected to a circadian rhythm, total RNA was obtained from the proximal jejunum taken from rats at different times during the day (09: 00, 12: 00, 16:00 and 21:00 h).…”

Section: Circadian Rhythm Of Glut5 Gene Expression In Jejunummentioning

confidence: 99%

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Regulation of GLUT5 gene expression in rat intestinal mucosa: regional distribution, circadian rhythm, perinatal development and effect of diabetes

Castelló

Gumà

Sevilla

et al. 1995

Biochemical Journal

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1. GLUT5 gene expression was studied in small intestine under a variety of conditions characterized by altered intestinal absorption of monosaccharides. 2. RNA-blotting studies showed that GLUT5 mRNA was abundantly expressed in rat and rabbit intestine and kidney, but it was not detected in heart or brown adipose tissue. GLUT5 mRNA levels were higher in the upper segments of the small intestine (duodenum and proximal jejunum) than in the lower segments (distal jejunum and ileum). 3. The intestinal expression of GLUT5 mRNA in rat proximal jejunum showed circadian rhythm. A 12-fold increase in GLUT5 mRNA levels was detected at the end of the light cycle and at the beginning of the dark cycle when compared with the early light period. In keeping with this, GLUT5 protein content in brush-border membranes was also increased at the beginning of the dark cycle compared with that in the light period. 4. In streptozotocin-induced diabetes an 80% increase in GLUT5 mRNA levels in mucosa from the proximal jejunum was detected under conditions in which enhanced intestinal absorption of monosaccharides has been reported. 5. The intestinal expression of GLUT5 mRNA showed regulation during perinatal development. Levels of GLUT5 mRNA were low during fetal life, increased progressively during the postnatal period and reached levels comparable with the adult state after weaning. Weaning on to a high-fat diet partially prevented the induction of GLUT5 gene expression. 6. Our results indicate that GLUT5 gene expression is tightly regulated in small intestine. Regulation involves maximal expression in the upper part of the small intestine, circadian rhythm, developmental regulation dependent on the fat and carbohydrate content in the diet at weaning and enhanced expression in streptozotocin-induced diabetes. Furthermore, changes observed in intestinal GLUT5 expression correlate with reported alterations in intestinal absorption of fructose. This suggests a regulatory role for GLUT5 in fructose uptake by absorptive enterocytes.

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Section: Discussionmentioning

confidence: 97%

Section: Circadian Rhythm Of Glut5 Gene Expression In Jejunummentioning

confidence: 99%

Regulation of GLUT5 gene expression in rat intestinal mucosa: regional distribution, circadian rhythm, perinatal development and effect of diabetes

Castelló

Gumà

Sevilla

et al. 1995

Biochemical Journal

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“…Although morphologi cal [1,2] and functional disorders [3,4] of the intestine are well documented in chronic renal failure, conflicting data have been reported concerning enzymatic activities of the small intestine in experimental uremic rats [5,6]. In a previous report on intestinal disaccharidase [7], we demonstrated that the circadian variations of these enzy matic activities may contribute to those conflicting re sults. However, we could not reveal the reason why these disaccharidase activities in uremic rats were not en hanced during their feeding time.…”

Section: Introductionmentioning

confidence: 75%

Effect of 1,25-Dihydroxyvitamin D<sub>3</sub> on Intestinal Disaccharidases in Uremic Rats

Inagaki¹,

Fujita²,

Inoue³

et al. 1986

Nephron

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The effect of 1,25-dihydroxyvitamin D₃ (1,25-D) on the activity of intestinal disaccharidases, maltase, sucrase, trehalase and lactase, was studied in five-sixths nephrectomized uremic rats. In uremic rats, maltase, sucrase and trehalase activities were lower than in sham-operated rats. Administration of 1,25-D produced significant improvement of maltase, sucrase, trehalase and lactase activities in uremic rats. These results suggest that activities of intestinal disaccharidases are reduced in uremic rats and these activities are normalized after 1,25-D administration.

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Lactose ‐ ein potentieller Ballaststoff. Zur Regulierung ihrer mikroökologischen Wirksamkeit im Intestialtrakt 4. Mitt. Ballaststoffwirkung der Lactose: Bewertung mit multivariaten statistischen Verfahren

Zunft

Schulze

1991

Nahrung

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The conditions and the intestinal processes responsible for the action of lactose as a potential dietary fibre are described. The beta-galactosidase activity in the rat caecum and colon is influenced by dietary factors: It declines with increasing lactose concentration and it rises with increasing protein and phosphate concentration in the diet. The enzyme activity correlates negatively with the content of lactose, and positively with the content of protein and phosphate in the chymus. The products of lactose hydrolysis are degraded by microbial glycolysis in caecum and colon. The glycolytic products are mainly absorbed and energetically utilized by the macroorganism. Phosphate stimulates the microbial metabolism and, therefore, accelerates the consumption of the energy substrate lactose. Mathematical optimization gives the necessary composition of the diet which causes an intended microecological effect. To minimize the chymus pH (5.1 in the colon ascendens; 4.6 in the colon descendens; 4.3 in the faeces) the lactose content of the diet has to be greater than or equal to 160 mumol/g, the protein content less than or equal to 10 mg/g, and the phosphate content less than or equal to 5.5 mumol/g. The minimal pH value depends to a greater extent on variations in the supply of protein and phosphorus with the diet whereas the response to changes in lactose concentration is less noticeable.

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Circadian Rhythms of Intestinal Disaccharidases in Experimental Uremia in Rats

Cited by 4 publications

References 10 publications

Regulation of GLUT5 gene expression in rat intestinal mucosa: regional distribution, circadian rhythm, perinatal development and effect of diabetes

Regulation of GLUT5 gene expression in rat intestinal mucosa: regional distribution, circadian rhythm, perinatal development and effect of diabetes

Effect of 1,25-Dihydroxyvitamin D<sub>3</sub> on Intestinal Disaccharidases in Uremic Rats

Lactose ‐ ein potentieller Ballaststoff. Zur Regulierung ihrer mikroökologischen Wirksamkeit im Intestialtrakt 4. Mitt. Ballaststoffwirkung der Lactose: Bewertung mit multivariaten statistischen Verfahren

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