Circadian rhythms in surface molecules of  rat blood lymphocytes

Pelegrı́, Carme; Vilaplana, Jordi; Castellote, Cristina; Rabanal, Manel; Franch, Àngels; Castell, Margarida

doi:10.1152/ajpcell.00084.2002

Cited by 44 publications

(27 citation statements)

References 41 publications

(39 reference statements)

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“…Exactly why we failed to replicate their Hct QTL is unknown at this time. One factor may have been time of day of collection, as Hct is known to show a circadian rhythm in rabbits and rats and (24,54) and probably in mice as well. In our work, we routinely kill and harvest between the hours of 0900 and 1200, i.e., 3-6 h after lights are turned on in the vivarium.…”

Section: Discussionmentioning

confidence: 99%

Systems genetic analysis of peripheral iron parameters in the mouse

Jones¹,

Beard²,

Gibson³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Iron homeostasis is one of the most critical functions in living systems. Too little iron can lead to anemia and tissue-specific disorders, such as splenomegaly. Excessive systemic iron is characteristic of hemochromatosis and is implicated in the brain in Parkinson's disease. With the exception of some single gene diseases like hemochromatosis, we know little about genetic-based, individual differences in iron-related parameters and their impact on biology. To model genetic control of iron homeostasis, we measured liver, spleen, and plasma iron concentrations, hematocrit and hemoglobin, transferrin saturation, and total iron-binding capacity in several BXD/Ty recombinant inbred mouse strains derived from C57BL/6 and DBA/2 progenitors. At 120 days of age, the animals were killed for iron analysis. All measures showed genetic-based variability consistent with polygenic influence. Analysis of principal components of the seven measures revealed three factors that we named availability, transport, and storage. Quantitative trait loci (QTL) analysis revealed one suggestive QTL on chromosome 5 for availability, two suggestive QTL (one on chromosome 1 and the other on chromosome 7) for transport, and one weak QTL on chromosome 2 for storage. The results show that iron homeostasis is a complex trait and is influenced by multiple genes.

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Section: Discussionmentioning

confidence: 99%

Systems genetic analysis of peripheral iron parameters in the mouse

Jones¹,

Beard²,

Gibson³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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show abstract

“…Circadian rhythmicity is revealed in most immune functions, including circulating cells, lymphocyte metabolism and transformability, circulating hormones and other substances that may exert various actions on different targets of the immune system, cytokines, receptors, and adhesion molecules, cell cycle events in health and cancer, reactions to antigen challenge, and disease etiology and symptoms [24][25][26] . Circadian changes in the circulation of T, B, or natural killer lymphocyte subsets in peripheral blood and in the density of epitope molecules at their surface, which may be related to cell reactivity to antigen exposure, have been reported [27] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Chronic Ethanol Feeding on 24-Hour Rhythms of Mitogenic Responses and Lymphocyte Subset Populations in Thymus and Spleen of Peripubertal Male Rats

Jiménez

Cardinali²,

Álvarez³

et al. 2005

Neuroimmunomodulation

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This work analyzes the effect of chronic ethanol feeding on the 24-hour variation of mitogenic responses and lymphocyte subset populations in thymus and spleen. Animals were maintained under a 12:12-hour light/dark photoperiod and they received a liquid diet for 4 weeks, starting on day 35 of life. The ethanol-fed group received a similar diet to controls except that maltose was isocalorically replaced by ethanol. Ethanol replacement provided 36% of the total caloric content of the diet. Rats were killed at 6 time intervals around the clock, beginning at Zeitgeber time (ZT) 1 (ZT 0 = lights on). Under ethanol intake the splenic and thymic weight decreased. In addition, mean values of the thymic, but not of the splenic T cell number decreased, and mean values of the thymic and splenic CD8+ and CD4+CD8+ number increased. Consequently, the thymic T/B ratio and the thymic and splenic CD4+/CD8+ ratio decreased in ethanol-fed rats. At the same time there was a significant increase in the response of the thymic cells to LPS. The ethanol diet modified the 24-hour rhythmicity of thymic and splenic T, B and CD4+CD8+ cells, thymic CD4+ and splenic CD8+ cells, thymic and splenic T/B and CD4+/CD8+ ratios, as well as of mitogenic responses in both tissues. Chronic ethanol administration presumably affects the endogenous clock that modulates the circadian variation of immune responsiveness in growing rats.

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“…Virtually all immunological variables investigated to date in animals and humans have been shown to display biological periodicity. In most of its components, the immune system shows regularly recurring, rhythmic variations in numerous frequencies, the circadian being the best known [43][44][45]. The results of our own studies on the circadian rhythmicity of immune parameters in rats receiving FCA or its vehicle are summarized in table 1.…”

Section: The Immune System Shows a Circadian Organizationmentioning

confidence: 99%

Circadian Disorganization in Experimental Arthritis

Cardinali

Esquifino²

2003

Neurosignals

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This review discusses the experimental evidence indicating that arthritis disrupts circadian organization, which was mainly derived from animal studies employing Freund’s complete mycobacterial adjuvant (FCA). The defense response to antigenic challenge, mediated in part by cytokines, includes changes in chronobiological central nervous system function, like depressed daily activity, superficial sleep or anorexia. Interferon (IFN)-γ receptors are detectable in the central circadian pacemaker, the hypothalamic suprachiasmatic nuclei, at a time when the capacity for photic entrainment of the pacemaker became established. The disruptive effects of the systemic injection of IFN on the circadian rhythms of locomotor activity, body temperature and clock-gene mRNA expression have been documented. In the last few years we have examined a number of immune and neuroendocrine circadian rhythms in FCA-injected rats, both in the preclinical phase of arthritis (2–3 days after FCA injection) as well as in the acute phase of the disease (18 days after FCA injection). In arthritic rats, the 24-hour organization of immune and neuroendocrine responses becomes altered. A hormonal pathway involving the circadian secretion of melatonin and a purely neural pathway including, as a motor leg, the autonomic nervous system innervating the lymph nodes were identified. The significant effects of the immune-mediated inflammatory response on the diurnal rhythmicity of adenohypophysial and hypophysiotropic hormones occurred in arthritic rats. Melatonin treatment prevented the alteration in 24-hour rhythms of serum ACTH, prolactin and luteinizing hormone in rats injected with FCA. In addition, melatonin pretreatment prevented the alteration in the 24-hour variation in hypothalamic serotonin and dopamine turnover during the preclinical phase of Freund’s adjuvant arthritis in rats. Some pinealectomy-induced immune changes in arthritic rats were also prevented by physiological concentrations of melatonin. Melatonin may play the role of an ‘internal synchronizer’ for the immune system.

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Circadian rhythms in surface molecules of rat blood lymphocytes

Abstract: Pelegrí, Carme, Jordi Vilaplana, Cristina Castellote, Manel Rabanal, À ngels Franch, and Margarida Castell. Circadian rhythms in surface molecules of rat blood lymphocytes.

Cited by 44 publications

References 41 publications

Systems genetic analysis of peripheral iron parameters in the mouse

Systems genetic analysis of peripheral iron parameters in the mouse

Effect of Chronic Ethanol Feeding on 24-Hour Rhythms of Mitogenic Responses and Lymphocyte Subset Populations in Thymus and Spleen of Peripubertal Male Rats

Circadian Disorganization in Experimental Arthritis

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