Circadian rhythms in metabolic variables in Caenorhabditis elegans

Migliori, María Laura; Simonetta, Sergio Hernan; Romanowski, Andrés; Golombék, Diego A.

doi:10.1016/j.physbeh.2011.01.026

Cited by 32 publications

(35 citation statements)

References 37 publications

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“…Over the last decade, there have been numerous reports of daily rhythms in the soil-dwelling nematode, C. elegans (6)(7)(8)(9)(10)(11)(12), although the connection between molecules and behavior has been lacking. Reasons for this may include a lack of known clock gene homologs as starting points, a number of low-amplitude behavioral rhythms, and the use of conditions that are far from ecological.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, protocols used for clock research on the nematode, C. elegans (6)(7)(8)(9)(10)(11)(12), have shown circadian rhythms in behaviors (e.g., locomotor activity, defecation, and pharyngeal pumping rate), metabolism (e.g., resistance to osmotic stress), or the expression of hundreds of genes without a connection to rhythmic behaviors. Clearly, taken together, one would conclude that the nematode has a circadian system.…”

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confidence: 99%

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Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Olmedo

O’Neill

Edgar

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks provide a temporal structure to processes from gene expression to behavior in organisms from all phyla. Most clocks are synchronized to the environment by alternations of light and dark. However, many organisms experience only muted daily environmental cycles due to their lightless spatial niches (e.g., caves or soil). This has led to speculation that they may dispense with the daily clock. However, recent reports contradict this notion, showing various behavioral and molecular rhythms in Caenorhabditis elegans and in blind cave fish. Based on the ecology of nematodes, we applied low-amplitude temperature cycles to synchronize populations of animals through development. This entrainment regime reveals rhythms on multiple levels: in olfactory cued behavior, in RNA and protein abundance, and in the oxidation state of a broadly conserved peroxiredoxin protein. Our work links the nematode clock with that of other clock model systems; it also emphasizes the importance of daily rhythms in sensory functions that are likely to impact on organism fitness and population structure.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Olmedo

O’Neill

Edgar

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…To construct a classical TTFL (5) with a negative feedback loop, at least one of these genes should cycle. Although there is no evidence from whole-nematode experiments showing that the PER homolog lin-42 is rhythmic at the mRNA level under LD or WC cycles (7,15), it remains possible that the gene cycles only in a subset of cells or that it could be regulated posttranslationally, as are peroxiredoxins, a conserved circadian marker in C. elegans and many other model organisms (7). Additionally, complex cytoplasmic regulations that occur by alternating species-specific heterodimerization partners and that modulate clock proteins function could be involved in the regulation of LIN-42 activity.…”

Section: Discussionmentioning

confidence: 99%

“…However, the study of circadian rhythms in this model is far from complete, and the mechanism underlying the molecular basis of the central pacemaker remains elusive. Numerous circadian cycles have been described in the nematode at different levels, including behavior (locomotor activity) (10)(11)(12), physiology (defecation rate, pharyngeal pumping rate, and olfaction) (13,14), metabolism (abiotic and biotic stress tolerance, food and oxygen consumption) (15,16), protein activity and regulation (14,17,18), and gene expression (19). Recent exhaustive bioinformatics searches have identified proteins of relatively high homology to clock components from other species (20).…”

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confidence: 99%

Circadian rhythms identified in Caenorhabditis elegans by in vivo long-term monitoring of a bioluminescent reporter

Goya

Romanowski

Caldart

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Circadian rhythms are based on endogenous clocks that allow organisms to adjust their physiology and behavior by entrainment to the solar day and, in turn, to select the optimal times for most biological variables. Diverse model systems-including mice, flies, fungi, plants, and bacteria-have provided important insights into the mechanisms of circadian rhythmicity. However, the general principles that govern the circadian clock of Caenorhabditis elegans have remained largely elusive. Here we report robust molecular circadian rhythms in C. elegans recorded with a bioluminescence assay in vivo and demonstrate the main features of the circadian system of the nematode. By constructing a luciferase-based reporter coupled to the promoter of the suppressor of activated let-60 Ras (sur-5) gene, we show in both population and single-nematode assays that C. elegans expresses ∼24-h rhythms that can be entrained by light/dark and temperature cycles. We provide evidence that these rhythms are temperature-compensated and can be re-entrained after phase changes of the synchronizing agents.In addition, we demonstrate that light and temperature sensing requires the photoreceptors LITE and GUR-3, and the cyclic nucleotidegated channel subunit TAX-2. Our results shed light on C. elegans circadian biology and demonstrate evolutionarily conserved features in the circadian system of the nematode.C. elegans | circadian rhythms | luminescence | temperature | light

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“…In addition, several studies have reported light-entrained circadian rhythms in behavior of C. elegans e.g. locomotor activity (Saigusa et al, 2002; Simonetta and Golombek, 2007), defecation and pharyngeal pumping rate (Migliori et al, 2011), metabolism e.g. resistance to osmotic stress (Kippert et al, 2002), and melatonin levels (Migliori et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Long-term imaging of circadian locomotor rhythms of a freely crawling C. elegans population

Winbush

Gruner

Hennig

et al. 2015

Journal of Neuroscience Methods

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Background Locomotor activity is used extensively as a behavioral output to study the underpinnings of circadian rhythms. Recent studies have required a populational approach for the study of circadian rhythmicity in Caenorhabditis elegans locomotion. New method We describe an imaging system for long-term automated recording and analysis of locomotion data of multiple free-crawling C. elegans animals on the surface of an agar plate. We devised image analysis tools for measuring specific features related to movement and shape to identify circadian patterns. Results We demonstrate the utility of our system by quantifying circadian locomotor rhythms in wild-type and mutant animals induced by temperature cycles. We show that 13 °C:18 °C (12:12 h) cycles are sufficient to entrain locomotor activity of wild-type animals, which persist but are rapidly damped during 13 °C free-running conditions. Animals with mutations in tax-2, a cyclic nucleotide-gated (CNG) ion channel, significantly reduce locomotor activity during entrainment and free-running. Comparison with existing method(s) Current methods for measuring circadian locomotor activity is generally restricted to recording individual swimming animals of C. elegans, which is a distinct form of locomotion from crawling behavior generally observed in the laboratory. Our system works well with up to 20 crawling adult animals, and allows for a detailed analysis of locomotor activity over long periods of time. Conclusions Our population-based approach provides a powerful tool for quantification of circadian rhythmicity of C. elegans locomotion, and could allow for a screening system of candidate circadian genes in this model organism.

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Circadian rhythms in metabolic variables in Caenorhabditis elegans

Cited by 32 publications

References 37 publications

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Circadian rhythms identified in Caenorhabditis elegans by in vivo long-term monitoring of a bioluminescent reporter

Long-term imaging of circadian locomotor rhythms of a freely crawling C. elegans population

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