Circadian rhythm of natural killer cell activity in vitiligo

Mozzanica, N.; Frigerio, U; Negri, Marco; Tadini, Gianluca; Villa, Maria Luisa; Mantovani, Marina; Finzi, A. F.

doi:10.1016/s0190-9622(89)70069-4

Cited by 23 publications

(7 citation statements)

References 11 publications

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“…Previous studies have shown an increase in the number of circulating NK cells in the blood of vitiligo patients [63], [64], [65], [66], [67]. Our present work has demonstrated the infiltration of NK cells in the skin microenvironment of melanocytes in both the lesional and non-lesional area of the skin of vitiligo patients.…”

Section: Discussionsupporting

confidence: 74%

Transcriptome Analysis Reveals Markers of Aberrantly Activated Innate Immunity in Vitiligo Lesional and Non-Lesional Skin

Broady

Huang

et al. 2012

PLoS ONE

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BackgroundVitiligo is characterized by the death of melanocytes in the skin. This is associated with the presence of T cell infiltrates in the lesional borders. However, at present, there is no detailed and systematic characterization on whether additional cellular or molecular changes are present inside vitiligo lesions. Further, it is unknown if the normal appearing non-lesional skin of vitiligo patients is in fact normal. The purpose of this study is to systematically characterize the molecular and cellular characteristics of the lesional and non-lesional skin of vitiligo patients.Methods and MaterialsPaired lesional and non-lesional skin biopsies from twenty-three vitiligo patients and normal skin biopsies from sixteen healthy volunteers were obtained with informed consent. The following aspects were analyzed: (1) transcriptome changes present in vitiligo skin using DNA microarrays and qRT-PCR; (2) abnormal cellular infiltrates in vitiligo skin explant cultures using flow cytometry; and (3) distribution of the abnormal cellular infiltrates in vitiligo skin using immunofluorescence microscopy.ResultsCompared with normal skin, vitiligo lesional skin contained 17 genes (mostly melanocyte-specific genes) whose expression was decreased or absent. In contrast, the relative expression of 13 genes was up-regulated. The up-regulated genes point to aberrant activity of the innate immune system, especially natural killer cells in vitiligo. Strikingly, the markers of heightened innate immune responses were also found to be up-regulated in the non-lesional skin of vitiligo patients.Conclusions and Clinical ImplicationsAs the first systematic transcriptome characterization of the skin in vitiligo patients, this study revealed previously unknown molecular markers that strongly suggest aberrant innate immune activation in the microenvironment of vitiligo skin. Since these changes involve both lesional and non-lesional skin, our results suggest that therapies targeting the entire skin surface may improve treatment outcomes. Finally, this study revealed novel mediators that may facilitate future development of vitiligo therapies.

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Section: Discussionsupporting

confidence: 74%

Transcriptome Analysis Reveals Markers of Aberrantly Activated Innate Immunity in Vitiligo Lesional and Non-Lesional Skin

Broady

Huang

et al. 2012

PLoS ONE

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“…Although several studies have shown quantitative abnormalities of NK cells in the peripheral blood of vitiligo patients [40,41], NK cells have not been localized in lesional or perilesional areas of vitiliginous skin [42]. In contrast, an important role of CTLmediated cytotoxicity in the destruction of MCs in human and SL vitiligo has been strongly suggested [25,27,43].…”

Section: Discussionmentioning

confidence: 99%

Apoptosis in feathers of Smyth line chickens with autoimmune vitiligo

Wang

Erf

2004

Journal of Autoimmunity

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“…82 Other experiments also downplay the role of natural killer and lymphokine-activated killer cells in the pathogenesis. 173 These include a decrease in T-helper cells, [174][175][176] an increase in natural killer cells, 175,177 circulating antibodies to surface antigens on melanocytes [178][179][180][181] and to certain melanoma cell lines, 182,183 aberrant expression of complement regulatory proteins, 184 an increase in met-enkephalin secretion, 185 a decrease in the expression of c-kit protein by melanocytes adjacent to lesional skin, 186,187 abnormal expression of MHC class II molecules and ICAM-1 by perilesional melanocytes, 188 and possible functional impairment of Langerhans cells. 173 These include a decrease in T-helper cells, [174][175][176] an increase in natural killer cells, 175,177 circulating antibodies to surface antigens on melanocytes [178][179][180][181] and to certain melanoma cell lines, 182,183 aberrant expression of complement regulatory proteins, 184 an increase in met-enkephalin secretion, 185 a decrease in the expression of c-kit protein by melanocytes adjacent to lesional skin, 186,187 abnormal expression of MHC class II molecules and ICAM-1 by perilesional melanocytes, 188 and possible functional impairment of Langerhans cells.…”

Section: Section 3 • the Epidermismentioning

confidence: 99%

Disorders of pigmentation

Weedon

2010

Weedon's Skin Pathology

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Circadian rhythm of natural killer cell activity in vitiligo

Cited by 23 publications

References 11 publications

Transcriptome Analysis Reveals Markers of Aberrantly Activated Innate Immunity in Vitiligo Lesional and Non-Lesional Skin

Transcriptome Analysis Reveals Markers of Aberrantly Activated Innate Immunity in Vitiligo Lesional and Non-Lesional Skin

Apoptosis in feathers of Smyth line chickens with autoimmune vitiligo

Disorders of pigmentation

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