Circadian rhythm generation in a glioma cell line

Noradrenaline Induces Clock Gene Per1 mRNA Expression in C6 Glioma Cells Through β2-Adrenergic Receptor Coupled With Protein Kinase A – cAMP Response Element Binding Protein (PKA–CREB) and Src-Tyrosine Kinase – Glycogen Synthase Kinase-3β (Src–GSK-3β)

“…A previous study has shown that C6 cells are a good tool for examining the underlying molecular machinery of clock gene expression because several clock genes are expressed and show the circadian oscillation after exposure to dexamethasone in these cells (20). In addition, we and another laboratory have examined various functions of astrocytes by using C6 cells (19,27).…”

“…Since these cells exhibit circadian oscillation in clockgene expression after exposure to several reagents (20), we felt it reasonable to try elucidating whether signaling cascades triggered after stimulation with NA also might affect clock-gene expression. As a result of testing this hypothesis, we found that treatment of C6 cells with NA led to transient increase in Per1 mRNA expression via β 2 -adrenergic receptors.…”

The disruption of circadian clockwork in differentiating cells from rat reproductive tissues as identified by in vitro real-time monitoring system

“…In the mammalian testicular and thymus tissues, the interstitial Leydig cells and the thymocytes (T lymphocytes) both undergo sequential stage-wise cellular differentiation (Touraine et al 1977, Penit & Vasseur 1988, Mendis-Handagama & Ariyaratne 2001. The in vitro realtime oscillation monitoring system is a valuable tool for the investigation of circadian clockwork in cultured cell lines (Fujioka et al 2006, Nakahata et al 2006. In the present study, the real-time monitoring system was employed to evaluate the clockwork in several types of differentiating cells originated from the reproductive tissues of transgenic rats that have been constructed with Per2 promoter-destabilized luciferase reporter gene.…”

Reduction of translation rate stabilizes circadian rhythm and reduces the magnitude of phase shift

“…A 3410 bp region upstream of the mouse Per2 sequence [20] was cloned into the KpnI/BglII sites of the pGL4.19 vector (Promega) to generate the mPer2::dLuc construct. Rat-1 cells was transfected with the mPer2::dLuc vector, and selected with 400 mg/ml G418 as previously described [21].…”