Circadian regulation of macromolecular complex turnover and proteome renewal

Seinkmane, Estere; Peak‐Chew, Sew Y.; Zeng, Aiwei; Rzechorzek, Nina Marie; Beale, Andrew D.; Wong, David; O’Neill, John S.

doi:10.1101/2022.09.30.509905

Cited by 5 publications

(4 citation statements)

References 138 publications

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“…BAM3 in Arabidopsis) the protein abundance tracks the transcript abundance ( 73 ). Circadian regulation of protein turnover occurs in eukaryotes ( 74, 75 ), and it is thought that circadian regulation of gene expression might maintain proteostasis in the presence of rhythmic protein degradation ( 76 ). This might explain why rhythms in protein abundance are less prevalent than rhythms of transcript abundance.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide circadian gating of a cold temperature response in bread wheat

Graham

Paajanen

Edwards

et al. 2022

Preprint

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Circadian rhythms coordinate the responses of organisms to their daily fluctuating environments, by establishing a temporal program of gene expression. This schedules aspects of metabolism, physiology, development and behaviour according to the time of day. Circadian regulation in plants is extremely pervasive, and is important because it underpins both productivity and seasonal reproduction. Circadian regulation extends to control of environmental responses through a regulatory mechanism known as circadian gating, whereby the circadian clock regulates the response to environmental cues such that the magnitude of response varies according to the time of day. Here, we show that there is genome-wide circadian gating of responses to cold temperatures in plants. By using bread wheat as an experimental model, we establish that circadian gating is crucial to the programs of gene expression that underlie the environmental responses of a crop of major socioeconomic importance. Furthermore, we identify that circadian gating of cold temperature responses are distributed unevenly across the three wheat subgenomes, which might reflect the geographical origins of the ancestors of modern wheat.

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Section: Discussionmentioning

confidence: 99%

Genome-wide circadian gating of a cold temperature response in bread wheat

Graham

Paajanen

Edwards

et al. 2022

Preprint

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“…In addition to differences in translation efficiency [51][52][53][54] , rhythmic degradation can explain rhythmic protein despite constant mRNA levels, as well as large delays between transcript and protein expression. 35,40,[55][56][57] Direct experimental evidence for rhythmic degradation of individual proteins is limited 41,45,58 , but our findings suggest widespread degradation rhythms 59 consistent with circadian rhythms in autophagy and protein ubiquitination. 60,61 For CRY1 -and to a lesser extent for CRY2 -the oscillation in protein stability depends on FBXL3, but the molecular basis is unclear.…”

Section: Discussionmentioning

confidence: 55%

Circadian period is compensated for repressor protein turnover rates in single cells

Gabriel,

del Olmo,

Widini

et al. 2024

Preprint

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Most mammalian cells possess molecular circadian clocks generating widespread rhythms, e.g. in transcript and protein abundance. While circadian clocks are robust to fluctuations in the cellular environment, little is known about how circadian period is compensated for fluctuating metabolic states. Here, we exploit the heterogeneity of single cells both in circadian period and metabolic state, governing protein stability, to study their interdependence without the need for genetic manipulation. We generated cells expressing key circadian proteins (CRY1/2 and PER1/2) as endogenous fusions with fluorescent proteins and simultaneously monitored circadian rhythms and degradation in thousands of single cells. We found that the circadian period is compensated for fluctuations in the turnover rates of circadian repressor proteins and uncovered possible mechanisms using a mathematical model. In addition, the stabilities of the repressor proteins are circadian phase-dependent and correlate with the circadian period in a phase-dependent manner, in contrast to the prevailing model.

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“…BAM3 in Arabidopsis) the protein abundance tracks the transcript abundance [ 72 ]. Circadian regulation of protein turnover occurs in eukaryotes [ 73 , 74 ], and it is thought that circadian regulation of gene expression might maintain proteostasis in the presence of rhythmic protein degradation [ 75 ]. This might explain why rhythms in protein abundance are less prevalent than rhythms of transcript abundance.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide circadian gating of a cold temperature response in bread wheat

Graham,

Paajanen,

Edwards

et al. 2023

PLoS Genet

View full text Add to dashboard Cite

Circadian rhythms coordinate the responses of organisms to their daily fluctuating environments, by establishing a temporal program of gene expression. This schedules aspects of metabolism, physiology, development and behaviour according to the time of day. Circadian regulation in plants is extremely pervasive, and is important because it underpins both productivity and seasonal reproduction. Circadian regulation extends to the control of environmental responses through a regulatory process known as circadian gating. Circadian gating is the process whereby the circadian clock regulates the response to an environmental cue, such that the magnitude of response to an identical cue varies according to the time of day of the cue. Here, we show that there is genome-wide circadian gating of responses to cold temperatures in plants. By using bread wheat as an experimental model, we establish that circadian gating is crucial to the programs of gene expression that underlie the environmental responses of a crop of major socioeconomic importance. Furthermore, we identify that circadian gating of cold temperature responses are distributed unevenly across the three wheat subgenomes, which might reflect the geographical origins of the ancestors of modern wheat.

show abstract

Circadian regulation of macromolecular complex turnover and proteome renewal

Cited by 5 publications

References 138 publications

Genome-wide circadian gating of a cold temperature response in bread wheat

Genome-wide circadian gating of a cold temperature response in bread wheat

Circadian period is compensated for repressor protein turnover rates in single cells

Genome-wide circadian gating of a cold temperature response in bread wheat

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