Circadian hormone secretion of enteroendocrine cells: implication on pregnancy status

Homeida, A.M.; Homeida, M; Al-Suhaimi, Ebtesam A.

doi:10.3389/fendo.2023.1106382

Cited by 2 publications

(2 citation statements)

References 132 publications

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“…Organized and timely gastrointestinal motility is required for normal GI function 5 . Whole gut transit (WGT) is a “leading‐edge” measurement of the amount of time it takes for the contents of the stomach to exit the anus in both preclinical 6 and clinical 7 studies.…”

Section: Introductionmentioning

confidence: 99%

“…1,4 Organized and timely gastrointestinal motility is required for normal GI function. 5 Whole gut transit (WGT) is a "leading-edge" measurement of the amount of time it takes for the contents of the stomach to exit the anus in both preclinical 6 and clinical 7 studies. In preclinical studies, this procedure is traditionally performed utilizing carmine red, a bright dye with the ability to change the color of stool.…”

Section: Introductionmentioning

confidence: 99%

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Circadian rhythm and whole gut transit in mice

Laible,

Wegner,

Knutson

et al. 2024

Neurogastroenterology Motil

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BackgroundIn preclinical studies whole gut transit (WGT) in mice is a gold‐standard “leading‐edge” approach that measures the time between orogastric gavage of carmine red and defecation of the first carmine red pellet. Transit studies in humans are performed during the active day because GI motility and transit are suppressed during the night. Since mice are nocturnal, WGT studies traditionally done during the day occur during their rest phase. How circadian rhythm affects WGT in mice is not known.MethodsWe used an automated approach for high temporal resolution uninterrupted testing of mouse WGT and activity. We housed wild‐type Bl6/C57 mice under the standard 12 h light–dark cycles. At 8 weeks, we performed carmine red orogastric gavage and assessed WGT during Light (rest) conditions. Then, we exposed mice to a reverse 12 h light–dark cycle for 2 weeks and tested them in the Dark (active) under red light conditions. Timelapse videos were analyzed to quantify activity and to timestamp all pellets, and multiple parameters were analyzed.Key ResultWhen complementary light cycle reversal experiments were performed, we found a significant increase in mouse activity when mice were tested during their Dark (active) phase, compared to their Light (rest) phase. In mice tested in the Active phase compared to the Rest phase, we found a significant acceleration in WGT, increased rate and total number of pellets produced, and more pellet clustering. These data show that the mice tested in the Active phase have important differences in activity that correlate with multiple alterations in gastrointestinal transit.Conclusion & InferencesDuring the Active phase mice have faster WGT, produce more pellets, and cluster their output compared to testing in the Rest phase. Like in humans, circadian rhythm is an important consideration for transit studies in mice, and a simple reverse light cycle approach facilitates further studies on the role of circadian rhythm in GI motility.

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Section: Introductionmentioning

confidence: 99%