Circadian Control of the Accumulation of mRNAs for Light- and Heat-Inducible Chloroplast Proteins in Pea (<i>Pisum sativum</i> L.)

Otto, Beate; Grimm, Bernhard; Ottersbach, Peter; Kloppstech, Klaus

doi:10.1104/pp.88.1.21

Cited by 60 publications

(33 citation statements)

References 31 publications

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“…Circadian expression has been described in many genes encoding products that function in photosynthetic light harvesting, such as CAB (Kay and Millar, 1993), or in photosynthetic carbon assimilation, such as RBCS (Spiller et al, 1987;Giuliano et al, 1988;Otto et al, 1988; and RCA (Martino-Catt and Ort, 1992;. Our data show that in mature plants CAT2 mRNA accumulation demonstrates robust circadian oscillations in continuous darkness and continuous light, persisting for at least 5 d in continuous light.…”

Section: Discussionmentioning

confidence: 51%

Interactions between Light and the Circadian Clock in the Regulation of CAT2 Expression in Arabidopsis

et al. 1994

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In Arabidopsis seedlings germinated and grown in continuous light, CATZ mRNA abundance peaks 1 d after imbibition, consistent with the role of catalase in detoxifying HzOz generated during the 8-oxidation of fatty acids stored in the seed. A second peak of CATZ mRNA abundance, of lower amplitude than the initial peak, appears 6 d after imbibition and may be associated with the development of photosynthetic competence and induction of photorespiration. This second peak in steady-state CATZ mRNA abundance is regulated by light and is not seen in etiolated seedlings. CATZ mRNA accumulation is induced by exposure to high-fluence blue or far-red light but not by red light. In addition, light induction is unaffected by severa1 mutations that block blue light-mediated inhibition of hypocotyl elongation (blul, blu2, blu3, hy4), suggesting phytochrome involvement. When etiolated seedlings are transferred to continuous white light, CATZ mRNA rapidly (within 30 min) accumulates. It is interesting that in these seedlings CAT2 mRNA abundance undergoes pronounced oscillations with a circadian (24 h) periodicity, indicating control by the endogenous circadian clock. No such oscillations are detected in CAT2 mRNA abundance in etiolated seedlings prior to illumination. Control of CATZ expression by the circadian clock is also seen in 5-week-old plants grown in a light-dark cycle and transferred either to continu o u~ dark or to continuous light; in continuous light the circadian oscillations in CATZ mRNA abundance persist for at least five circadian cycles, indicating the robustness of this circadian rhythm.

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Section: Discussionmentioning

confidence: 51%

Interactions between Light and the Circadian Clock in the Regulation of CAT2 Expression in Arabidopsis

et al. 1994

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“…Oscillations in the level of Elip transcripts have been already reported in other species (Otto et al, 1988;Po¨tter and Kloppstech, 1993) and attributed to the presence of a circadian control. This type of regulation is actually predictable, since the genomic sequence of both Elip genes contains circadian regulatory elements (the ''CAANNN-NATC'' and ''GATA'' motives) in the 5¢-upstream region, which are present and conserved in clock controlled Lhc genes (Piechulla et al, 1998).…”

Section: Discussionmentioning

confidence: 93%

“…However, since it was reported that in barley and pea the level of Elip transcript fluctuates diurnally (Otto et al, 1988, Po¨tter and Kloppstech, 1993, we included in the experiment the analysis of transcripts and proteins in leaves maintained in the growing chamber at a light intensity of 120 lE/ m 2 s (LL). Samples were collected from LL and HL plants at the same times.…”

Section: Effect Of Light and Temperature On Elips Transcription And Amentioning

confidence: 99%

Mutational and expression analysis of ELIP1 and ELIP2 in Arabidopsis thaliana

et al. 2005

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Plants exposed to photoinhibitory conditions respond by accumulation of the early light-induced proteins (ELIPs) with a potential photoprotective function. In Arabidopsis thaliana two genes (Elip1 and Elip2) encode for two ELIP proteins: evidence exists that the two genes are differentially regulated but their precise function is unclear. Mutants null for one or the other Elip gene can help in elucidating ELIPs role and here we describe the expression profile of ELIP1 and ELIP2, and the phenotype of such null mutants. Both ELIPs accumulate during greening of etiolated seedlings and in mature plants the transcripts fluctuate diurnally without protein accumulation. Steady-state transcript level of both genes increases in response to high light with transcription of Elip1 much more sensitive than that of Elip2 to increasing irradiation at 22°C. At 4°C instead Elip2 is strongly transcribed even at growing light. Furthermore, only ELIP1 accumulates under high light at 22°C while both proteins accumulate at 4°C. These results indicate the existence of a differential regulation of ELIPs expression in response to light or chilling stress with mechanisms active either at transcriptional and post-transcriptional level. Phenotypically, the mutants behave as the wild type as far as sensitivity to light-or light and cold-induced short-term photoinhibition, while both ELIPs are necessary to ensure a high rate of chlorophyll accumulation during deetiolation in continuous high light.

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“…In pea, under heat-shock conditions, the abundance of mRNA corresponding to some low molecular mass hsps was found to vary during the day, indicating a circadian control of their accumulation (20). Our own studies of a second hsp homolog, P. nil hsp83, show that its expression is induced by light (6).…”

Section: Identification Of the Transcription Initiationmentioning

confidence: 97%

Structure and Light-Induced Expression of a Small Heat-Shock Protein Gene of Pharbitis nil

et al. 1992

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To isolate genes that are regulated by a photoperiod that promotes flowering in Pharbitis nil, a cDNA library representing mRNA of induced cotyledons was screened by differential hybridization. The DNA sequence of one cDNA clone isolated by this approach, clone 12L, showed homology to plant small heat-shock protein (hsp) genes. P. nil genomic clones hybridizing to clone 12L were isolated, and the DNA sequences of two P. nil small hsp (shsp) genes, shsp-1 and shsp-2, were determined. The derived amino acid sequences of shsp-1 and shsp-2 showed maximum homology to the 17.9-kD soybean hsp, a member of the class 11 cytoplasmic hsps found in plants. A The physiological processes in floral initiation can be divided into three steps: photoperiodic perception by cotyledons, translocation of induced floral stimulus to the shoot tip where floral initiation takes place, and evocation that results in the commitment of the meristem to form flowers. RNA synthesis in cotyledons, in response to the inductive photoperiod, has been suggested as a necessary part of floral induction in P. nil (1, 31). Recently, more direct molecular approaches have been applied to the study of changes in gene expression associated with the photoperiodic induction of flowering. Both qualitative and quantitative differences were found between translatable mRNAs isolated from cotyledons of plants kept in continuous light and from cotyledons of plants that received an inductive dark period (16).To isolate genes whose expression in P. nil cotyledons is regulated by a photoperiod that leads to flowering, we used a differential screening procedure. We report the isolation, sequence, and expression of one of the genes isolated by this approach. We demonstrate that this gene is a member of a multigene family and can potentially encode a low molecular mass hsp4. Expression of one member of this family is regulated by heat shock and by light, and expression of a related member is regulated only by heat shock. MATERIALS AND METHODS PlantsPharbitis nil plants were grown at 230C for 5 d in coolwhite fluorescent light (145 gE m-2 s-1). At this time, they were subjected to an inductive dark period of 12 or 14 h and were subsequently kept in light for 2 h. The cotyledons were excised, and RNA was extracted as described below. Plants that received a red light night break were exposed to 20 min of red light at the 7th hour of the dark treatment.

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Circadian Control of the Accumulation of mRNAs for Light- and Heat-Inducible Chloroplast Proteins in Pea (Pisum sativum L.)

Cited by 60 publications

References 31 publications

Interactions between Light and the Circadian Clock in the Regulation of CAT2 Expression in Arabidopsis

Interactions between Light and the Circadian Clock in the Regulation of CAT2 Expression in Arabidopsis

Mutational and expression analysis of ELIP1 and ELIP2 in Arabidopsis thaliana

Structure and Light-Induced Expression of a Small Heat-Shock Protein Gene of Pharbitis nil

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