Circadian clock as possible protective mechanism to pollution induced keratinocytes damage

Benedusi, Mascia; Frigato, Elena; Beltramello, Mattia; Bertolucci, Cristiano; Valacchi, Giuseppe

doi:10.1016/j.mad.2017.08.017

Cited by 17 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of note, this trend was also noticed at later time point up to 2 hr. These results suggest that clock synchronization provides a more prompt ability of the cells to increase NRF2 confirming what has been already shown in previous work by Benedusi et al [12].…”

Section: Resultssupporting

confidence: 92%

“…On the basis of our previous results [12] showing the association between circadian system and oxidative stress, we explored the crosstalk between NRF2 and NF-κB in clock-synchronized and arrhythmic human keratinocytes exposed to O 3 .…”

Section: Resultsmentioning

confidence: 99%

“…Ozone treatment was performed in previously LPStreated-synchronized and control cells 18 hours after dex treatment in accordance with our previous work [12].…”

Section: Methodsmentioning

confidence: 99%

“…Among the multiplicity of physiological systems hypothesized to have a role in the onset of pathologies in case of circadian disruption, there are redox-sensitive defensive pathways and inflammatory machinery [10,11]. The effects of dysregulated circadian system on cellular defensive enzyme activities were previously reported, and recent investigations have further elucidated the molecular mechanisms that are possibly the link between the circadian clock and the maintenance of cellular redox homeostasis [12]. Moreover, a growing collection of data focus on the deep interconnection between altered redox homeostasis and inflammatory pathways (OxInflammation), since it seems that the imbalance of those two critical pathways can contribute to initiation, development, and progression of several disorders [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Concerning the skin, our group has previously demonstrated that in human keratinocytes, which comprise~95% of the cells within the epidermis, endogenous circadian clock is involved in the cellular response to oxidative stress. In particular, after the exposure to ozone (O 3 ), one of the most toxic outdoor pollutants, the clock-synchronized keratinocytes exhibit a more efficient antioxidant response compared to arrhythmic ones, attested by a quicker activation of the master defensive cellular transcription factor, nuclear factor erythroid 2-related factor 2 (NRF2) [12].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Frigato

Benedusi

Guiotto

et al. 2020

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Circadian rhythms are biological oscillations that occur with an approximately 24 h period and optimize cellular homeostasis and responses to environmental stimuli. A growing collection of data suggests that chronic circadian disruption caused by novel lifestyle risk factors such as shift work, travel across time zones, or irregular sleep-wake cycles has long-term consequences for human health. Among the multiplicity of physiological systems hypothesized to have a role in the onset of pathologies in case of circadian disruption, there are redox-sensitive defensive pathways and inflammatory machinery. Due to its location and barrier physiological role, the skin is a prototypical tissue to study the influence of environmental insults induced OxInflammation disturbance and circadian system alteration. To better investigate the link among outdoor stressors, OxInflammation, and circadian system, we tested the differential responses of keratinocytes clock synchronized or desynchronized, in an in vitro inflammatory model exposed to O 3 . Being both NRF2 and NF-κB two key redox-sensitive transcription factors involved in cellular redox homeostasis and inflammation, we analyzed their activation and expression in challenged keratinocytes by O 3 . Our results suggest that a synchronized circadian clock not only facilitates the protective role of NRF2 in terms of a faster and more efficient defensive response against environmental insults but also moderates the cellular damage resulting from a condition of chronic inflammation. Our results bring new insights on the role of circadian clock in regulating the redoxinflammatory crosstalk influenced by O 3 and possibly can be extrapolated to other pollutants able to affect the oxinflammatory cellular processes.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

“…Ozone treatment was performed in previously LPStreated-synchronized and control cells 18 hours after dex treatment in accordance with our previous work [12].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Frigato

Benedusi

Guiotto

et al. 2020

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

Involvement of the TREK‐1 channel in human alveolar cell membrane potential and its regulation by inhibitors of the chloride current

Canella

Martini

Cavicchio³

et al. 2019

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl− channel inhibitors: (4‐[(2‐butyl‐6,7‐dichloro‐2‐cyclopentyl‐2,3‐dihydro‐1‐oxo‐inden‐5‐yl)oxy] butanoic acid (DCPIB) and 9‐anthracenecarboxylic acid (9‐AC) on the total current in a type II pneumocytes (A549 cell line) model by patch clamp, immunocytochemical, and gene knockdown techniques. We noted that DCPIB and 9‐AC promote the activation of K conductance. In fact, they significantly increase the intensity of the current and shift its reversal potential to values more negative than the control. By silencing outward rectifier channel in its anoctamin 6 portion, we excluded a direct involvement of Cl− ions in modulation of IK and, by means of functional tests with its specific inhibitor spadin, we identified the TREK‐1 channel as the presumable target of both drugs. As the activity of TREK‐1 has a key role for the correct functioning of the alveolar epithelium, the identification of DCPIB and 9‐AC molecules as its activators suggests their possible use to build new pharmacological tools for the modulation of this channel.

show abstract

Air Pollution and the Skin Health

Neto

Leite

Rossi

et al. 2023

Dermatology in Public Health Environments

View full text Add to dashboard Cite

Circadian clock as possible protective mechanism to pollution induced keratinocytes damage

Cited by 17 publications

References 37 publications

Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Circadian Clock and OxInflammation: Functional Crosstalk in Cutaneous Homeostasis

Involvement of the TREK‐1 channel in human alveolar cell membrane potential and its regulation by inhibitors of the chloride current

Air Pollution and the Skin Health

Contact Info

Product

Resources

About