Circadian and Seasonal Variation in Human Preovulatory Follicular Fluid Melatonin Concentration*

Rönnberg, Lars; Kauppila, Antti; Leppäluoto, Juhani; Martikainen, Hannu; Vakkuri, Olli

doi:10.1210/jcem-71-2-493

Cited by 143 publications

(99 citation statements)

References 15 publications

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“…This is consistent with the earlier reports on human and rat demonstrating the presence of MTNR1A and MTNR1B in granulosa cells and luteal cells (Soares et al 2003). The presence of high levels of melatonin in follicular fluid in human and binding sites in the ovary (Brzezinski et al 1987, Ronnberg et al 1990, Niles et al 1999 and, now, the presence of MTNR1A and MTNR1B in ovary suggest that melatonin directly acts as a modulator of ovarian functions.…”

Section: Discussionsupporting

confidence: 92%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

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The aim of the present study was to evaluate the seasonal variation in serum melatonin levels and their relationship to the changes in the serum progesterone level, ovarian steroidogenesis, and embryonic development during two successive pregnancies of Cynopterus sphinx. Circulating melatonin concentrations showed two peaks; one coincided with the period of low progesterone synthesis and delayed embryonic development, whereas the second peak coincided with regressing corpus luteum. This finding suggests that increased serum melatonin level during November-December may be responsible for delayed embryonic development by suppressing progesterone synthesis. The study showed increased melatonin receptors (MTNR1A and MTNR1B) in the corpus luteum and in the utero-embryonic unit during the period of delayed embryonic development. The in vitro study showed that a high dose of melatonin suppressed progesterone synthesis, whereas a lower dose of melatonin increased progesterone synthesis by the ovary. The effects of melatonin on ovarian steroidogenesis are mediated through changes in the expression of peripheral-type benzodiazepine receptor, P450 side chain cleavage enzyme, and LH receptor proteins. This study further showed a suppressive impact of melatonin on the progesterone receptor (PGR) in the utero-embryonic unit; this effect might contribute to delayed embryonic development in C. sphinx. The results of the present study thus suggest that a high circulating melatonin level has a dual contribution in retarding embryonic development in C. sphinx by impairing progesterone synthesis as well as by inhibiting progesterone action by reducing expression of PGR in the utero-embryonic unit. Reproduction (2009) 138 935-944

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Section: Discussionsupporting

confidence: 92%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

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“…Regarding the effects of melatonin, the concentrations of melatonin in human preovulatory follicular fluid are almost three-fold higher than serum levels (Ronnberg et al, 1990). There are some evidences that intrafollicular melatonin serves to protect both the oocytes and the steroid-secreting granulosa cells from toxic oxygen and nitrogen-based byproducts during the ovulatory process (Sugino, 2008).…”

Section: Resultsmentioning

confidence: 99%

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum -FCS) at 39°C and 5% CO 2 in air. After 24 hours of culture in MM with 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH; 10 -9 M MEL) or 10 -9 M MEL, 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10 -9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM. Key words: comet assay, embryos, gonadotropins, in vitro fertilization, melatonin Melatonina no meio de maturação não melhorou as taxas de maturação dos ovócitos, de desenvolvimento embrionário e a fragmentação do DNA dos embriões bovinos RESUMO: Melatonin (MEL) atua como um potente redutor de radicais livres. Efeito direto da MEL na função gonadal também foi observado. Existem poucos estudos relacionados ao efeito da MEL durante a maturação no desenvolvimento embrionário in vitro. Avaliou-se a adição de MEL no meio de maturação (sem gonadotrofinas) nas taxas de maturação nuclear e de desenvolvimento embrionário e na incidência de fragmentação do DNA nos embriões produzidos in vitro. Complexos cumulus-ovócitos foram aspirados de ovários provenientes de matadouros e cultivados em meio de maturação (Meio TCM-199 suplementado com 10% de soro fetal bovino) a 39°C e 5% CO 2 em ar. Após 24 horas de cultivo em meio de maturação com 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH; 10 -9 M de MEL ou 10 -9 M de MEL, 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH, os ovócitos foram corados com Hoechst 33342 para avaliação da taxa de maturação nuclear. A fragmentação do DNA dos blastocistos foi avaliada pela técnica do Cometa. Não houve efeito da MEL adicionada ao meio de maturação, com ou sem gonadotrofinas, nas taxas de maturação nuclear, clivagem e blastocistos. Os percentuais variaram entre 88% a 90%, 85% a 88% e 42% a 46%, respectivamente. O grau de fragmentação do DNA também não foi afetado pela adição de MEL ao meio de matur...

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“…In birds, melatonin also regulates circadian rhythm, hibernation, feeding pattern, thermoregulation, and neuroendocrine functions (Courtillot et al, 2010). Melatonin is found in ovarian follicular fluid (Rönnberg et al, 1990), suggesting a direct effect of this hormone on ovarian function. The effects of melatonin on ovarian function vary with tissue structure, cell type, and with the fact whether the species is a seasonal or non-seasonal breeder (Soares et al, 2003).…”

mentioning

confidence: 99%

Genetic effects of melatonin receptor genes on chicken reproductive traits

Li¹,

Zhang²,

Smith³

et al. 2013

Czech J. Anim. Sci.

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ABSTRACT:The melatonin receptors are G protein-coupled receptors (GPCR) that bind melatonin. Three types of melatonin receptors have been cloned. The MTNR1A (or Mel1A or MT1) and MTNR1B (or Mel1B or MT2) receptor subtypes are present in humans and other mammals, while an additional melatonin receptor subtype MTNR1C (or Mel1C or MT3) has been identified in amphibians and birds. Previous research has shown that the three common melatonin receptors regulate physiological processes, including seasonal reproduction and ovarian physiology. However, whether or not any polymorphisms of the different melatonin receptor subtypes are associated with reproductive traits in chickens is not known. In this study, we performed candidate gene analysis to identify single-nucleotide polymorphisms (SNPs) in the MTNR1A, MTNR1B, and MTNR1C genes in the Erlang Mountain Chicken population. SNP discovery was achieved by sequencing pooled DNA samples. Direct PCR-sequencing, PCR-SSCP/PCR-sequencing, and PCR-RFLP method were used to genotype the MTNR1A, MTNR1B, and MTNR1C genes, respectively. The GLM Procedure was used to estimate the statistical significance of association between genotypes at each locus and reproductive traits of chickens. In a sample of 460 chickens, four novel polymorphisms (JQ249890:g.384T>C, JQ249891:g.387T>C, JQ249894:g.63C>T, and JQ249896:g.294G>A) were detected in the melatonin receptor genes MTNR1A, MTNR1B, and MTNR1C, respectively. A statistically significant association (P < 0.01) was found between two SNPs (MTNR1A SNP, MTNR1C SNP) and reproductive traits: egg number at 300 days of age (EN) and age at first egg (AFE).

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Circadian and Seasonal Variation in Human Preovulatory Follicular Fluid Melatonin Concentration*

Cited by 143 publications

References 15 publications

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Genetic effects of melatonin receptor genes on chicken reproductive traits

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