Circ_CUX1/miR-130b-5p/p300 axis for parathyroid hormone-stimulation of Runx2 activity in rat osteoblasts: A combined bioinformatic and experimental approach

Krishnan, R.; Sadu, Lakshana; Akshaya, R.L.; Gomathi, K.; Saranya, I.; Das, Udipt Ranjan; Satishkumar, Sneha; Selvamurugan, N.

doi:10.1016/j.ijbiomac.2022.11.176

Cited by 9 publications

(4 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miRNAs have the potential to target more than one gene; similarly, one miRNA can be targeted or sponged by more than one circRNA [ [76] , [77] , [78] ]. For example, miR-130b-5p was also sponged by circ_CUX1 under PTH treatment in osteoblasts [ 79 ]. This type of regulation is essential for regulating various cellular activities under physiological conditions [ 80 , 81 ], and dysfunction in the interaction of these ncRNAs may lead to pathological conditions [ [82] , [83] , [84] ].…”

Section: Discussionmentioning

confidence: 99%

Circ_ST6GAL1-mediated competing endogenous RNA network regulates TGF-β1-stimulated matrix Metalloproteinase-13 expression via Runx2 acetylation in osteoblasts

Saranya,

Akshaya,

Gomathi

et al. 2024

Non-coding RNA Research

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Circ_ST6GAL1-mediated competing endogenous RNA network regulates TGF-β1-stimulated matrix Metalloproteinase-13 expression via Runx2 acetylation in osteoblasts

Saranya,

Akshaya,

Gomathi

et al. 2024

Non-coding RNA Research

View full text Add to dashboard Cite

“…The knockdown of circ_CUX1 led to the upregulation of miR-130b-5p, thereby reducing the expression level of p300. 53…”

Section: -3p Was Responsible For the Increased Expression Of Atf3mentioning

confidence: 99%

Transforming Growth Factor-β1-mediated Regulation of circ_DISP3 and ATF3 in Human Triple-negative Breast Cancer Cells

Mohan,

Bharathy,

Induja

et al. 2023

Gene Expr

View full text Add to dashboard Cite

Background and objectives: Transforming growth factor-beta 1 (TGF-β1) stimulates activating transcription factor 3 (ATF3), and it is required for the expression of cell proliferation, invasion, and bone metastasis genes in human breast cancer cells. Noncoding RNAs have a crucial role in both physiological and pathological circumstances in the expression of genes. We previously determined the direct targeting of ATF3 by miR-4638-3p, downregulated by TGF-β1 in human triple-negative breast cancer cells (MDA-MB231). This study identified the molecular mechanism of downregulating ATF3 targeting miR-4638-3p via circRNA in MDA-MB231 cells. Methods:In-silico analyses were used to identify the list of circRNAs targeting miR-4638-3p. RT-qPCR was performed to determine the expression of shortlisted circRNAs in MDA-MB231 cells. Transient transfection and western blot analyses were carried out to identify the functional role of circ_DISP3 in MDA-MB231 cells. A dual-luciferase reporter assay was used to identify the direct binding of circ_DISP3 and miR-4638-3p. Results:There was an inverse correlation between the expression of circ_DISP3 and miR-4638-3p in these cells. Antisense oligosmediated silencing of circ_DISP3 restored the function of miR-4638-3p, resulting in the downregulation of ATF3 in MDA-MB231 cells. Furthermore, a luciferase reporter assay identified the direct binding of circ_DISP3 toward miR-4638-3p in these cells.Conclusions: TGF-β1 influenced circ_DISP3 to sponge miR-4638-3p, promoting ATF3 expression in MDA-MB231 cells. Silencing this circRNA caused the restoration of miR-4638-3p activity toward targeting ATF3 in these cells. Hence, we suggest that the

show abstract

“…The continuous closed‐loop structure from the 3′ and 5′ ends of circRNAs, in contrast to traditional linear RNAs, makes circRNAs more stable and less prone to degradation by RNA exonucleases (Misir et al., 2022). CircRNAs have complicated biological functions and participate in various cellular processes, including cell differentiation and proliferation (Fan et al., 2023; Geng et al., 2019; Krishnan et al., 2023; Wang, Wu, et al., 2022; Zhang et al., 2019). Recently, circRNAs have been considered functional molecules capable of modulating the osteogenic differentiation of MSCs.…”

Section: Introductionmentioning

confidence: 99%

Circ‐ZNF236 mediates stem cells from apical papilla differentiation by regulating LGR4‐induced autophagy

Xiao,

Chen,

et al. 2024

Int Endodontic J

View full text Add to dashboard Cite

AimHuman stem cells from the apical papilla (SCAPs) are an appealing stem cell source for tissue regeneration engineering. Circular RNAs (circRNAs) are known to exert pivotal regulatory functions in various cell differentiation processes, including osteogenesis of mesenchymal stem cells. However, few studies have shown the potential mechanism of circRNAs in the odonto/osteogenic differentiation of SCAPs. Herein, we identified a novel circRNA, circ‐ZNF236 (hsa_circ_0000857) and found that it was remarkably upregulated during the SCAPs committed differentiation. Thus, in this study, we showed the significance of circ‐ZNF236 in the odonto/osteogenic differentiation of SCAPs and its underlying regulatory mechanisms.MethodologyThe circular structure of circ‐ZNF236 was identified via Sanger sequencing, amplification of convergent and divergent primers. The proliferation of SCAPs was detected by CCK‐8, flow cytometry analysis and EdU incorporation assay. Western blotting, qRT‐PCR, Alkaline phosphatase (ALP) and Alizarin red staining (ARS) were performed to explore the regulatory effect of circ‐ZNF236/miR‐218‐5p/LGR4 axis in the odonto/osteogenic differentiation of SCAPs in vitro. Fluorescence in situ hybridization, as well as dual‐luciferase reporting assays, revealed that circ‐ZNF236 binds to miR‐218‐5p. Transmission electron microscopy (TEM) and mRFP‐GFP‐LC3 lentivirus were performed to detect the activation of autophagy.ResultsCirc‐ZNF236 was identified as a highly stable circRNA with a covalent closed loop structure. Circ‐ZNF236 had no detectable influence on cell proliferation but positively regulated SCAPs odonto/osteogenic differentiation. Furthermore, circ‐ZNF236 was confirmed as a sponge of miR‐218‐5p in SCAPs, while miR‐218‐5p targets LGR4 mRNA at its 3′‐UTR. Subsequent rescue experiments revealed that circ‐ZNF236 regulates odonto/osteogenic differentiation by miR‐218‐5p/LGR4 in SCAPs. Importantly, circ‐ZNF236 activated autophagy, and the activation of autophagy strengthened the committed differentiation capability of SCAPs. Subsequently, in vivo experiments showed that SCAPs overexpressing circ‐ZNF236 promoted bone formation in a rat skull defect model.ConclusionsCirc‐ZNF236 could activate autophagy through increasing LGR4 expression, thus positively regulating SCAPs odonto/osteogenic differentiation. Our findings suggested that circ‐ZNF236 might represent a novel therapeutic target to prompt the odonto/osteogenic differentiation of SCAPs.

show abstract

Circ_CUX1/miR-130b-5p/p300 axis for parathyroid hormone-stimulation of Runx2 activity in rat osteoblasts: A combined bioinformatic and experimental approach

Cited by 9 publications

References 90 publications

Circ_ST6GAL1-mediated competing endogenous RNA network regulates TGF-β1-stimulated matrix Metalloproteinase-13 expression via Runx2 acetylation in osteoblasts

Circ_ST6GAL1-mediated competing endogenous RNA network regulates TGF-β1-stimulated matrix Metalloproteinase-13 expression via Runx2 acetylation in osteoblasts

Transforming Growth Factor-β1-mediated Regulation of circ_DISP3 and ATF3 in Human Triple-negative Breast Cancer Cells

Circ‐ZNF236 mediates stem cells from apical papilla differentiation by regulating LGR4‐induced autophagy

Contact Info

Product

Resources

About