Circ_0110251 overexpression alleviates IL-1β-induced chondrocyte apoptosis and extracellular matrix degradation by regulating miR-3189-3p/SPRY1 axis in osteoarthritis

Zhang, Yawei; Zheng, Hengheng; Li, Baitong

doi:10.1080/08916934.2022.2027917

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…MiR-3189-3p inhibition ameliorated IL-1β-induced chondrocyte apoptosis and ECM degradation, while SPRY1 silencing rescued these effects. Circ_0110251 overexpression alleviates IL-1β-induced chondrocyte apoptosis and extracellular matrix degradation by regulating the miR-3189-3p/SPRY1 axis in osteoarthritis 15 . During osteogenic differentiation of hBM-MSCs, Guo et al 16 .…”

Section: Discussionmentioning

confidence: 99%

Plasma miR-3192-5p is a Potential biomarker associated with spinal tuberculosis patients

Dai,

Li,

Xiang

et al. 2024

Preprint

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Background. Spinal tuberculosis (STB) accounts for approximately 50% of all bone and joint tuberculosis cases, and the understanding of the molecular mechanism of spinal tuberculosis remains limited. Accurate biomarkers are needed to diagnose spinal tuberculosis, which will help manage the incidence of spinal tuberculosis. The gold standard for the diagnosis of spinal tuberculosis requires biopsies of the lesions, which often cause the lesions to spread or delay the timing of anti-tuberculosis therapy. Therefore, there is a pressing need to develop noninvasive diagnostic tools. As a candidate diagnostic marker of spinal tuberculosis, circulating microRNAs (miRNAs) have the characteristics of easy detection, good stability, and strong tissue specificity. Methods. The gene expression database was utilized to compare the expression of miRNAs between patients diagnosed with spinal tuberculosis and undiagnosed patients, and the interaction between miRNAs and target genes was analysed to explain their expression and function. A protein‒protein interaction (PPI) network was further constructed. In bioinformatics analysis, RT‒qPCR was used to verify the expression of miRNAs in the BMSC cell line. A receiver operating characteristic (ROC) curve was constructed by using plasma miRNAs from 12 patients with spinal tuberculosis and 12 healthy controls, and its clinical diagnostic value was evaluated. Results. We identified six novel miRNAs as potential candidate diagnostic biomarkers for patients with spinal tuberculosis. In addition, the predicted target genes provide insight into the molecular mechanisms underlying spinal tuberculosis. Conclusion. There was a stable and significant difference in the expression of miR-3192-5p between STB and healthy controls. Our findings may provide reliable candidate biomarkers for the precise diagnosis and individualized treatment of STB and the development of further clinical applications in STB.

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Section: Discussionmentioning

confidence: 99%

Plasma miR-3192-5p is a Potential biomarker associated with spinal tuberculosis patients

Dai,

Li,

Xiang

et al. 2024

Preprint

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“…MiR-3189 is contained in the intron of Growth Differentiation Factor 15 (GDF15) [ 17 , 30 , 31 ] and its expression in various tissues is either low or undetectable [ 16 , 17 , 30 – 33 ]. Others and we have found a potent anti-cancer activity exerted by miR-3189-3p mimic on several types of tumors [ 17 , 31 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Others and we have found a potent anti-cancer activity exerted by miR-3189-3p mimic on several types of tumors [ 17 , 31 , 34 ]. Recent literature suggests a potential role for miR-3189-3p in Parkinson’s disease [ 32 ], in osteoarthritis [ 33 ], and in endoplasmic reticulum stress response in various types of cancer cells [ 35 ]. Nevertheless, the function of miR-3189-3p in normal and pathologic conditions remains largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of miR-3189-3p-mediated inhibition of c-MYC translation in triple negative breast cancer

et al. 2022

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Background Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by the lack of estrogen receptor, progesterone receptor, and HER2. Our lab previously characterized miR-3189-3p as a microRNA with potent anti-cancer activity against glioblastoma. Here, we hypothesized a similar activity in TNBC cells. As miR-3189-3p is predicted to target a variety of RNA binding proteins, we further hypothesized an inhibitory effect of this miRNA on protein synthesis. Methods MDA-MB-231 and MDA-MB-468 cells were used to investigate the effect of miR-3189-3p on cell proliferation, migration, and invasion. TGCA database was used to analyze the expression of miR-3189-3p, c-MYC, 4EPB1, and eIF4E in breast cancer. Western blotting and RT-qPCR assays were used to assess the expression of selected proteins and RNAs after transfections. Results Although c-MYC is not a predicted gene target for miR-3189-3p, we discovered that c-MYC protein is downregulated in miRNA-treated TNBC cells. We found that the downregulation of c-MYC by miR-3189-3p occurs in both normal growth conditions and in the absence of serum. The mechanism involved the direct inhibition of eIF4EBP1 by miR-3189-3p. Additionally, we found that miR-3189-3p could negatively affect cap-independent translation mediated by internal ribosome entry sites (IRES) or by m6A. Finally, miR-3189-3p sensitized TNBC cells to doxorubicin. Conclusion Overall, results indicated that miR-3189-3p exerts its anti-tumor activity through targeting translational regulatory proteins leading to an impairment in c-MYC translation, and possibly other oncogenic factors, suggesting that miR-3189-3p, alone or in combination, could be a valuable therapeutic approach against a malignancy with few treatment options.

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Role and Application of Biomolecules for Regeneration of Cartilage Tissue

Nakamichi

Fujii

Asahara

2023

Cartilage: From Biology to Biofabrication

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Circ_0110251 overexpression alleviates IL-1β-induced chondrocyte apoptosis and extracellular matrix degradation by regulating miR-3189-3p/SPRY1 axis in osteoarthritis

Cited by 5 publications

References 41 publications

Plasma miR-3192-5p is a Potential biomarker associated with spinal tuberculosis patients

Plasma miR-3192-5p is a Potential biomarker associated with spinal tuberculosis patients

Mechanisms of miR-3189-3p-mediated inhibition of c-MYC translation in triple negative breast cancer

Role and Application of Biomolecules for Regeneration of Cartilage Tissue

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