circ_0089153 exacerbates breast cancer cells proliferation and metastasis via sponging miR‐2467‐3p/E2F6

Abstract

The role of circ_0089153 in breast cancer (BCa) malignancy development was explored. circ_0089153 expression in BCa was analyzed by Gene Expression Omnibus database. Clinical tissues were obtained from 90 BCa patients. Cell counting kit-8 assay, 5-ethnyl-2 deoxyuridine assay and colony formation experiment were applied for proliferation detection. Wound healing assay and Transwell experiment were used for migration and invasion detection. Dual luciferase reporter gene assay, RNA immunoprecipitation assay and RNA pull-down assay were conducted. In vivo growth and metastasis of BCa cells were performed. Quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry were applied for RNAs and proteins expression. The up-modulated circ_0089153 indicated an unfavorable survival of BCa patients. circ_0089153 knockdown attenuated BCa cells proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) (P < .01). circ_0089153 was miR-2467-3p sponge. Low miR-2467-3p expression indicated a worse survival of BCa patients. miR-2467-3p overexpression reduced BCa cells proliferation, migration, invasion and EMT (P < .05). circ_0089153 enhanced BCa cells proliferation, migration, invasion and EMT by sponging miR-2467-3p (P < .05). E2F6 was directly suppressed by miR-2467-3p. E2F6 high expression in BCa patients associated with worse survival. circ_0089153 knockdown suppressed in vivo BCa cells growth and lung metastasis (P < .01). circ_0089153 was an oncogene in breast cancer, which enhanced proliferation and metastasis through sponging miR-2467-3p/ E2F6. circ_0089153 was suggested to be a potential target for BCa target treatment.

The pan‐cancer analysis of the oncogenic role of FAM72A as a BRCA prognostic biomarker and immunotherapeutic target

“…Tumors are the public enemy of people all over the world, with a progressive increase in prevalence and mortality 16–18 . Tumor heterogeneity implies unsatisfying survival, posing high demands for individualized treatment regimens 19 .…”

“…We then performed cell cycle distribution experiments to investigate whether Tumors are the public enemy of people all over the world, with a progressive increase in prevalence and mortality. [16][17][18] Tumor heterogeneity implies unsatisfying survival, posing high demands for individualized treatment regimens. 19 Therefore, biomarkers contributing to early diagnosis and determining prognosis have become a crucial area of exploration.…”

Critical Roles of Circular RNA in Tumor Metastasis via Acting as a Sponge of miRNA/isomiR

“…There are many circRNAs that can regulate EMT and metastasis and further contribute to the carcinogenic process, and the circular RNA-associated ceRNA network involved in biological processes may be quite crucial in the occurrence and development of cancer. For example, circ_0047303 may be a potential key regulator to mediate the upregulation of key angiogenesis-related genes, including HIF-1, EIF4E2, and VEGFA in TNBC, via sponging the tumor-suppressive miRNAs [64], circ_0000518 promotes breast cancer progression through the miRNA-1225-3p/SRY-box transcription factor 4 pathway, and it is oncogenic in BC [65], circ_0089153 is an oncogene in breast cancer, which enhances proliferation and metastasis via sponging miR-2467-3p/E2F6 [66], circ_IRAK3 exerts a promoting effect on BC progression by modulating the miR-603/KIF2A axis [67], circRNA_000554 up-regulates ZFP36 by sponging miR-182 [68], circ_RPPH1 up-regulates ARHGAP1 via sponging miR-542-3p [69], circ_ZFR up-regulates FABP7 by sponging miR-223-3p [70]. Hsa-circ-0061825 (circ-TFF1) contributes to BC via targeting miR-326/TFF1 [71], and circ-NOT2 can prevent breast cancer invasion, migration and EMT by regulating twist family BHLH transcription factor via targeting miR-409-3p/Twist1 [72].…”

Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR‐511‐3p in non‐small cell lung cancer

“…CircRNAs are ideal candidates for future therapeutic interventions due to their high stability properties endowed by their closed structures 20 . Accumulating shreds of evidence have indicated the association of circRNAs in cancer development, including NSCLC 21,22 . For instance, circ‐SATB2 elevated PDL1 expression via repressing miR‐326 activity, leading to facilitating cell malignant transformation in NSCLC 23 .…”