Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression

Abstract

The study was aimed at deciphering the function and mechanism of circ_0081001 in osteosarcoma (OS). In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was utilized for quantifying circ_0081001, miR-494-3p, and BTB domain and CNC homolog 1 (BACH1) mRNA expressions in OS tissues and cells. Cell counting kit-8 (CCK-8) assay, together with 5-Ethynyl-2'-deoxyuridine (EdU) assay, was performed for evaluating cell proliferation; the alterations in apoptosis were analyzed utilizing flow cytometry; Transwell assay was conducted for examining cell migration and invasion; moreover, Western blot was utilized for the quantification of BACH1 protein expression; bioinformatics, dual-luciferase reporter gene, and RNA-binding protein immunoprecipitation assays were executed for validating the binding relationships between circ_0081001 and miR-494-3p, and between miR-494-3p and BACH1. As shown, circ_0081001, whose expression was elevated in OS tissues, had a negative association with miR-494-3p expression and a positive correlation with BACH1 expression. After circ_0081001 was overexpressed, the proliferation, migration, and invasion of OS cells were boosted but the apoptosis was reduced, whereas miR-494-3p exhibited opposite effects. The binding sites between circ_0081001 and miR-494-3p, and between miR-494-3p and the 3’UTR of BACH1 were experimentally verified. In conclusion, circ_0081001/miR-494-3p/BACH1 axis promoted the malignant biological behaviors of OS cells.

Identification of Candidate MicroRNA-mRNA Subnetwork for Predicting the Osteosarcoma Progression by Bioinformatics Analysis

“…Moreover, the function of hsa-miR-494 as a tumor inhibitor restricts the proliferation and development of OS. The observably decreased hsa-miR-494-3p was also observed in OS specimens by previous studies [25,26]. In our work, hsa-miR-494 expression was appreciably suppressed in OS Computational and Mathematical Methods in Medicine compared with control group, and OS patients with downregulated hsa-miR-494 have a worse prognosis, indicating that our results are consistent with the consequences of previous investigations.…”

Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia

“…Deficiency of circDNA2 down-regulated CCDC6 level and suppressed the proliferative capacities of GC cells by sponging miR-149-5p [34] . Hsa_circ_0081001 (circCYP51A1) is found as an oncogenic factor in OS [35] . Another study reported that circ_0081001 contributed to methotrexate sensitivity through the regulation of miR-494-3p/transglutaminase 2 pathway [36] .…”

Six MicroRNA Prognostic Models for Overall Survival of Lung Adenocarcinoma

“…e miR-494-3p is upregulated in endometrial cancer, glioma, retinoblastoma, and hepatocellular carcinoma, which promotes cancer progression by regulating the PTEN/PI3K/AKT pathway [33][34][35][36]. While miR-494-3p is downregulated in synovial sarcoma, prostate cancer, osteosarcoma, and oral squamous cell carcinoma, and acts as a tumor suppressor miRNA [37][38][39][40]. Furthermore, miR-494-3p was associated with a new tumor driver of lung cancer.…”