Circ_0080425 inhibits cell proliferation and fibrosis in diabetic nephropathy via sponging miR‐24‐3p and targeting fibroblast growth factor 11

Liu, Huifang; Wang, Xin; Wang, Zheng-Yu; Li, Lingzhi

doi:10.1002/jcp.29329

Cited by 58 publications

(37 citation statements)

References 36 publications

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“…Moreover, miR-24-3p can be regulated by several lncRNAs in numerous types of cancer, including hepatocellular carcinoma (34), prostate cancer (35) and lung cancer (36). miR-24-3p is also sponged by circ_0080425 in diabetic nephropathy, leading to cell proliferation and fibrosis inhibition (37). However, the sponge regulation of miR-24-3p by circRNAs in NSCLC remains unknown.…”

Section: Discussionmentioning

confidence: 99%

hsa_circ_0058357 acts as a ceRNA to promote non‑small cell lung cancer progression via the hsa‑miR‑24‑3p/AVL9 axis

2021

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Abnormal circular RNAs (circRNAs) are associated with biological processes in cancer; however, the function of circRNAs remains largely unknown in non-small cell lung cancer (NSCLC). The present study aimed to investigate the role of hsa_circ_0058357 on the progression of NSCLC. Cell proliferation, migration and apoptosis were determined using Cell Counting Kit-8, Transwell and flow cytometry assays, respectively. Gene [circRNA and microRNA (miR)] and protein expression levels were determined via reverse transcription-quantitative PCR and immunoblotting. A luciferase assay was employed to detect the binding of miR-24-3p with AVL9 cell migration associated (AVL9), while a cancer xenograft model was established to evaluate cancer growth in vivo. The results demonstrated that hsa_circ_0058357 was highly expressed in human NSCLC tissues and NSCLC cells compared with para-cancerous tissues and human bronchial epithelial (HBE) cells, respectively. Knockdown of hsa_circ_0058357 significantly suppressed cell viability, migration and tumor growth, while it promoted apoptosis in NSCLC cells. As a competing endogenous RNA, hsa_circ_0058357 knockdown contributed to the increase of miR-24-3p expression in NSCLC cells. Of note, overexpression of miR-24-3p markedly abolished the exogenous hsa_circ_0058357-induced excessive proliferation, migration and apoptosis resistance of NSCLC cells. Mechanistically, as a signaling molecule in late secretory pathway, AVL9 was also expressed at a high level in NSCLC tissues and cells, which could be directly suppressed by miR-24-3p. In the tumor tissues, along with growth inhibition, hsa_circ_0058357 knockdown also mediated the elevation of miR-24-3p and the reduction of AVL9. Thus, it was suggested that hsa_circ_0058357 may be a crucial regulation factor in NSCLC by sponging hsa-miR-24-3p, leading to a decrease in miR-24-3p expression, and subsequent increase in AVL9 expression. Therefore, hsa_circ_0058357 may serve as a potential target for diagnosis and gene therapy for NSCLC.

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Section: Discussionmentioning

confidence: 99%

hsa_circ_0058357 acts as a ceRNA to promote non‑small cell lung cancer progression via the hsa‑miR‑24‑3p/AVL9 axis

2021

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“…in gastric cancer, the knockdown of hsa_circ_0001368 results in accelerated tumor growth in vivo and may act as a cerna to sponge mir-6506-5p and play a tumor-suppressive role (28). liu et al (29) suggested that circ_0080425 functions as sponge, harboring mir-24-3p, which inhibits cell proliferation and fibrosis in dn by targeting fibroblast growth factor 11. Furthermore, Yao et al (30) demonstrated that circ_0000285 aggravates podocyte injury through sponging mir-654-3p and activating MaPK6 in dn.…”

Section: Discussionmentioning

confidence: 99%

A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR‑101b by targeting TGFβRI

Mou¹,

Chenv²,

Zhou³

et al. 2020

Mol Med Rep

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circular rnas (circrnas) have crucial roles in various diseases; however, the mechanisms of action underlying circrnas in the occurrence and development of diabetic nephropathy (dn) remains largely unknown. The present study investigated the differentially expressed circrnas in the dn mice kidney cortex using circrna sequencing and elucidated the role of circrnas in mesangial cells. it was revealed that 40 circrnas were unconventionally expressed, including 18 upregulated circrnas and 22 downregulated circRNAs. Furthermore, circ_0000491 levels were significantly augmented in both dn mice and high glucose (HG, 30 mM)-induced mouse mesangial cells (MeS13 cells). Knockdown of circ_0000491 significantly suppressed the increase of vimentin, fibronectin and α-smooth muscle actin, as well as collagen type i, iii and iV, whilst reversing the decrease of e-cadherin in HG-induced MeS13 cells. it was further revealed that circrna_0000491 sponged mir-101b and that mir-101b directly targets TGFβri. in addition, the expression levels of mir-101b were negatively associated with the transcriptional level of circrna_0000491 and mir-101b inhibitors reversed the suppression of extracellular matrix (ecM)-associated protein synthesis mediated by knocking-down circrna_0000491. in conclusion, the present study investigated the circrna_0000491/mir-101b/TGFβri axis in ecM accumulation and fibrosis-associated protein expression levels of mesangial cells, which suggested that circRNA_0000491 may be beneficial for the development of an effective therapeutic target for dn.

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“…circRNA hsa_circ_0054633 was found to be elevated in pre‐diabetes and type 2 diabetes patients' peripheral blood and might serve as a diagnostic biomarker, which provides new ideas for the diagnosis of type 2 diabetes 17 . Some circRNAs have a potential role in diabetic retinopathy, 18,19 diabetic nephropathy, 20 diabetes‐related coronary artery disease, 21 diabetes‐related depression 22 and gestational diabetes mellitus 23 . However, the roles of circRNAs in T1D mellitus are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Circulating circular RNAs profiles associated with type 1 diabetes

Luo

Deng

Xie

et al. 2020

Diabetes Metabolism Res

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Aims Circular RNAs (circRNAs) have recently been shown to exert important effects in human diseases. However, the roles of circRNAs in type 1 diabetes (T1D) are largely unknown. This study is to identify the circRNA expression profiles in the peripheral blood of patients with T1D and predict their potential regulatory mechanisms and coding potential. Methods CircRNA expression profiles were detected by Arraystar human circRNA microarray. With real‐time PCR validation, multiple bioinformatics approaches were used to explore their biological functions, construct the circRNA‐miRNA‐mRNA interactions, and predict circRNA coding potential. Results A total of 93 differentially expressed circular transcripts were identified in T1D compared with controls, among which 30 were upregulated, and 63 were downregulated. Two circRNAs were identified to have significant differences by RT‐PCR. Gene ontology analysis enriched terms such as cellular protein metabolic process, cytoplasm and zinc ion binding. The proposed molecular functions of these differentially expressed circRNAs, including cellular protein metabolic process, cytoplasm, and binding, may contribute to T1D. The most enriched pathways for these circRNAs were involved in protein processing in the endoplasmic reticulum. Hsa_circ_0072697 may be involved in 50 circRNA‐miRNA‐mRNA signalling pathways related to diabetes, such as circ_0072697‐miR‐15a‐UBASH3A network. Furthermore, hsa_circ_0071224, hsa_circ_0002437, hsa_circ_0084429, hsa_circ_0072697, and hsa_circ_0000787 in T1D were considered to have the most coding potential involved in the pathogenesis of T1D. Conclusions These results showed that circRNAs are aberrantly expressed in the peripheral blood of patients with T1D and may play potential actions by interactions with miRNA and circRNA‐derived peptides in the development of T1D.

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Circ_0080425 inhibits cell proliferation and fibrosis in diabetic nephropathy via sponging miR‐24‐3p and targeting fibroblast growth factor 11

Cited by 58 publications

References 36 publications

hsa_circ_0058357 acts as a ceRNA to promote non‑small cell lung cancer progression via the hsa‑miR‑24‑3p/AVL9 axis

hsa_circ_0058357 acts as a ceRNA to promote non‑small cell lung cancer progression via the hsa‑miR‑24‑3p/AVL9 axis

A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR‑101b by targeting TGFβRI

Circulating circular RNAs profiles associated with type 1 diabetes

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