Circ_0075829 facilitates the progression of pancreatic carcinoma by sponging miR‐1287‐5p and activating LAMTOR3 signalling

Zhang, Xudong; Xue, Cailin; Chen, Xiaoping; Zhou, Zhao; Fu, Yue; Xiong, Yin; Wu, Si-Pei; Gong, Yu; Liu, Yi; Zhu, Chen; Qin, Xihu

doi:10.1111/jcmm.16089

Cited by 24 publications

(21 citation statements)

References 38 publications

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“…Current evidence suggests that the CeRNA network composed of circRNA‐miRNA‐mRNA plays a key role in regulating carcinogenesis and development and regulates the biological characteristics of tumors. 18 Circ_0075829 has been found to regulate LAMTOR3 through the ceRNA network to promote pancreatic cancer progression, 7 suggesting that both ceRNA networks and noncoding RNAs (lncRNA, miRNA, and circRNA) can be further explored for cancer mechanisms based on LAMTOR3.…”

Section: Discussionmentioning

confidence: 99%

“… 6 In addition, circ_0075829 has been found to promote pancreatic cancer progression through miR‐1287‐5P/LAMTOR3 signaling pathways. 7 In breast cancer research, miR‐20a could promote the apoptosis of breast cancer cells by regulating the MTOR signaling pathway via sponging LAMTOR3. 8 However, the expression and biological function of LAMTOR3 in KIRC remains unknown, warranting further exploration.…”

Section: Introductionmentioning

confidence: 99%

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LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Gong

et al. 2022

Clinical Laboratory Analysis

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Objective The objective of the study was to investigate the expression of LAMTOR3 in kidney renal clear cell carcinoma (KIRC) and its clinical significance. Methods The expression of LAMTOR3 in KIRC and its relationship with clinical features were analyzed using the UALCAN online database. The results were verified using KIRC gene chip data and clinical specimens. The prognosis of KIRC patients was analyzed with the GEPIA2 database. GO, KEGG, and GSEA analyses were conducted to analyze the possible molecular mechanism of LAMTOR3 in KIRC. Immunohistochemical (IHC) and hematoxylin and eosin (H&E) staining were used for histopathological detection. Results UALCAN database analysis showed that LAMTOR3 expression in KIRC was significantly lower than in normal kidney tissues and correlated with the clinical stage, pathological grade, and tumor genotype ( p < .05). GSE53757 dataset analysis consistently showed that the expression of LAMTOR3 in KIRC was significantly lower than in normal kidney tissues ( p < .01). GEPIA2 database analysis indicated that patients with low LAMTOR3 expression had poor overall and disease‐free survival rates. GSEA analysis suggested that LAMTOR3 positively regulated the citrate cycle and drug metabolism cytochrome P450 and negatively regulated folate biosynthesis and olfactory transduction. The expression of LAMTOR3 in KIRC was also significantly correlated with immune cell infiltration. Finally, IHC showed that LAMTOR3 expression in the KIRC tissues was lower than in the adjacent normal tissues. Conclusion LAMTOR3 expression is significantly lower in KIRC. LAMTOR3 may be a potential marker for KIRC diagnosis and therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Gong

et al. 2022

Clinical Laboratory Analysis

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“…Hao et al confirmed that circ_0007534 can directly interact with miR-625 and miR-892b [73], which implied that circ_0007534/ miR-625/miR-892b regulation axis might contribute to the development of PDAC. circ_0013912, circ_0000069, circ_0071036, circ_0075829, circ_0099999 could also facilitate the proliferative, migratory and invasive rates of PaCa cells through sponging miR-7-5p, miR-144, miR-489, miR-1287-5p and miR-335-5p, respectively [74][75][76][77][78]. circSFMBT1 serves as a miR-330-5p sponge and also promotes PaCa growth and metastasis via regulating miR-330-5p/ PAK1 axis [79].…”

Section: Circrnas Play Key Roles In Pancreatic Cancermentioning

confidence: 99%

Circular RNAs as novel potential biomarkers for pancreatic cancer

Liu¹,

Li²,

Ma³

et al. 2021

J. Cancer

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Pancreatic cancer (PaCa) is the fourth leading cause of cancer-related deaths in the United States, and the vast majority of these malignancies are pancreatic ductal adenocarcinomas (PDAC), but there is still a lack of early detection biomarkers for PaCa. Unlike linear RNAs, circRNAs form covalently closed continuous loops and can act as mammalian gene regulators. They may be diagnostic or predictive biomarkers for some tumors, also be novel potential therapeutic targets in different diseases. This review focuses on (1) the biogenesis of circRNAs, RNA binding proteins (RBPs) and complementary sequences of circRNAs; (2) the characteristics of circRNAs which allow them to interact with miRNAs; (3) the roles of circRNAs playing in the regulation of gene expression, cell behavior and cancer, and their potential role as novel biomarkers and therapeutic targets in pancreatic cancer.

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“…Numerous researches have shown that circRNAs can act as competitive endogenous RNAs (ceRNAs) by sponging miRNAs [ 10 ]. MiR-1287-5p functioned as a tumor inhibitor in various cancers, including breast cancer [ 11 ], colorectal cancer [ 12 ], and pancreatic carcinoma [ 13 ]. In non-small cell lung cancer (NSCLC), miR-1287 was proved to be sponged by circ_0016760 and circ_0026134 [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Circ_0047921 acts as the sponge of miR-1287-5p to stimulate lung cancer progression by regulating proliferation, migration, invasion, and glycolysis of lung cancer cells

Yang

Yao

et al. 2022

World J Surg Onc

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Background Lung cancer is a common respiratory system disease caused by multiple factors. Circular RNAs (circRNAs) play vital roles in tumorigenesis, including lung cancer. This study aimed to clarify the role and underlying molecular mechanisms of circ_0047921 in lung cancer. Methods Real-time quantitative polymerase chain reaction (RT-qPCR) was used to assess the expression levels of circ_0047921, La-related protein 1 (LARP1), and miR-1287-5p. Cell proliferation was analyzed by CCK-8 and EdU assays. Transwell assay was used to assess migration and invasion. Western blot assay was employed to quantify protein expression. Glycolysis ability of cell was determined by measuring glucose consumption and lactate production with matched kits. The relationship between miR-1287-5p and circ_0047921 or LARP1 was confirmed by dual-luciferase reporter assay. In addition, a xenograft model was established to clarify the functional role of circ_0047921 in vivo. Results Circ_0047921 was highly expressed in lung cancer tissues and cells. Circ_0047921 downregulation repressed proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) and glycolysis in lung cancer cells. Circ_0047921 targeted miR-1287-5p to deplete miR-1287-5p expression. The effects caused by circ_0047921 downregulation were reversed by miR-1287-5p inhibition. In addition, LARP1 was a target of miR-1287-5p, and circ_0047921 could directly interact with miR-1287-5p to increase the expression of LARP1. The effects caused by circ_0047921 downregulation were also reversed by LARP1 overexpression. Circ_0047921 silencing impeded the growth of tumor in vivo. Conclusion Circ_0047921 was overexpressed in lung cancer, and circ_0047921 targeted miR-1287-5p to modulate LARP1 expression, thereby facilitating the development of lung cancer. Trial registration The present study was approved by the ethical review committee of The First People’s Hospital of Chenzhou, Southern Medical University with reference no. 20210106.

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Circ_0075829 facilitates the progression of pancreatic carcinoma by sponging miR‐1287‐5p and activating LAMTOR3 signalling

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 24 publications

References 38 publications

LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Circular RNAs as novel potential biomarkers for pancreatic cancer

Circ_0047921 acts as the sponge of miR-1287-5p to stimulate lung cancer progression by regulating proliferation, migration, invasion, and glycolysis of lung cancer cells

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