Circ_0044556 Promotes the Progression of Colorectal Cancer via the miR-665-Dependent Expression Regulation of Diaphanous Homolog 1

Ma, Xu; Deng, Chao

doi:10.1007/s10620-021-07310-w

Cited by 11 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is predicted that the miRNAs targeted by hsa_circRNA_100833 are low expressed and can inhibit the proliferation and growth of CRC cells. Ma & Deng (2021) confirmed that miR-665 plays a role in inhibiting the proliferation, migration and invasion of CRC cells. MiR-448 ( Zhang et al, 2019 ) has also been confirmed to play a tumor suppressive role by promoting cell apoptosis.…”

Section: Discussionmentioning

confidence: 69%

Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer

Liu

Jiang

et al. 2022

PeerJ

View full text Add to dashboard Cite

Background Circular RNAs (circRNAs) have been discovered in colorectal cancer (CRC), but there are few reports on the expression distribution and functional mining analysis of circRNAs. Methods Differentially expressed circRNAs in CRC tissues and adjacent normal tissues were screened and identified by microarray and qRT-PCR. ROC curves of the six circRNAs were analyzed. A series of bioinformatics analyses on differentially expressed circRNAs were performed. Results A total of 207 up-regulated and 357 down-regulated circRNAs in CRC were screened, and three top up-regulated and down-regulated circRNAs were chosen to be verified in 33 pairs of CRCs by qRT-PCR. 6 circRNAs showed high diagnostic values (AUC = 0.6860, AUC = 0.8127, AUC = 0.7502, AUC = 0.9945, AUC = 0.9642, AUC = 0.9486 for hsa_circRNA_100833, hsa_circRNA_103828, hsa_circRNA_103831 and hsa_circRNA_103752, hsa_circRNA_071106, hsa_circRNA_102293). A circRNA-miRNA-mRNA regulatory network (cirReNET) including six candidate circRNAs, 19 miRNAs and 210 mRNA was constructed, and the functions of the cirReNET were predicted and displayed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on these mRNAs and protein-protein interaction (PPI) network of the hub genes acquired by string and CytoHubba. Conclusion A cirReNET containing potential diagnostic and predictive indicators of CRCs and several critical circRNA-miRNA-mRNA regulatory axes (cirReAXEs) in CRC were mined, and may provide a novel route to study the mechanism and clinical targets of CRC.

show abstract

Section: Discussionmentioning

confidence: 69%

Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer

Liu

Jiang

et al. 2022

PeerJ

View full text Add to dashboard Cite

show abstract

“…It is predicted that the miRNAs targeted by hsa_circRNA_100833 are low expressed and can inhibit the proliferation and growth of CRC cells. Ma et al confirmed that miR-665 (Ma & Deng 2021) plays a role in inhibiting the proliferation, migration and invasion of CRC cells. MiR-448 (Zhang et al 2019) has also been confirmed to play a tumor suppressive role by promoting cell apoptosis.…”

Section: Discussionmentioning

confidence: 96%

Peer Review #1 of "Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer (v0.2)"

Mutlu¹

2022

View full text Add to dashboard Cite

“…Furthermore, the expression level of miR‐665 was negatively correlated with hsa_circ_0081069 in CRC tissues and cells, suggesting that they play opposite roles in CRC. Ma et al reported that miR‐665 negatively regulates Homolog 1 to suppress tumor cells growth in CRC 26 . Moreover, miR‐665 impaired the self‐renewal capability of tumor stem cells inn CRC by suppressing the expression of signal transducer and activator Of transcription 3 (STAT3) 27 .…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs are implicated in the regulation of tumor progression via translation and degradation of target mRNAs 24,25 . For instance, Ma et al reported that miR‐665 negatively regulates Homolog 1 to suppress tumor cells growth in CRC 26 . Moreover, miR‐665 impaired the self‐renewal capability of tumor stem cells inn CRC by suppressing the expression of signal transducer and activator Of transcription 3 (STAT3) 27 .…”

Section: Introductionmentioning

confidence: 99%

“…24 , 25 For instance, Ma et al reported that miR‐665 negatively regulates Homolog 1 to suppress tumor cells growth in CRC. 26 Moreover, miR‐665 impaired the self‐renewal capability of tumor stem cells inn CRC by suppressing the expression of signal transducer and activator Of transcription 3 (STAT3). 27 Nevertheless, the regulatory mechanism for miR‐665 expression in CRC still needs to be clarified.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hsa_hsa_circ_0081069 promotes the progression of colorectal cancer through sponging miR‐665 and regulating E2F3 expression

Xie

Jin

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background Circular RNAs (circRNAs) have been implicated in the initiation and development of various cancers. This study explored the potential contribution of hsa_hsa_circ_0081069 in the progression of colorectal cancer (CRC). Methods The gene expression was analyzed by qRT‐PCR. Functional roles of hsa_circ_0081069 were examined by shRNA‐mediated silencing using CCK‐8 proliferation assay, Transwell migration and invasion assay, tube formation assay. The tumorigenesis and metastasis of CRC cells were assess in a xenograft mouse model. Results Hsa_circ_0081069 was significantly upregulated in CRC tissues and cells. Hsa_circ_0081069 knockdown suppressed the proliferation, migration and invasion in CRC cells, as well as the angiogenesis. Silencing hsa_circ_0081069 also impaired the tumorigenesis of CRC cells in a xenograft mouse model. Furthermore, miR‐665 was identified as an interacting partner of hsa_circ_0081069, which was negatively regulated by hsa_circ_0081069. miR‐665 targeted the mRNA of E2F3 to suppress its expression. We further demonsatred that miR‐665/E2F3 axis mediated the functional role of hsa_circ_0081069 in regulating the malignant phenotype of CRC cells. Conclusions Collectively, our study suggests that hsa_circ_0081069 could serve as a prognostic marker in progression of CRC. Targeting hsa_circ_0081069 and miR‐665/E2F3 axis could serve as potential therapeutic strategies for CRC treatment.

show abstract

Circ_0044556 Promotes the Progression of Colorectal Cancer via the miR-665-Dependent Expression Regulation of Diaphanous Homolog 1

Cited by 11 publications

References 28 publications

Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer

Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer

Peer Review #1 of "Potential and functional prediction of six circular RNAs as diagnostic markers for colorectal cancer (v0.2)"

Hsa_hsa_circ_0081069 promotes the progression of colorectal cancer through sponging miR‐665 and regulating E2F3 expression

Contact Info

Product

Resources

About