Circ_0005526 contributes to interleukin-1β-induced chondrocyte injury in osteoarthritis via upregulating transcription factor 4 by interacting with miR-142-5p

Abstract

Circular RNAs (circRNAs) can regulate the progression of osteoarthritis (OA) via serving as competing endogenous RNAs (ceRNAs). This work was performed for functional research of circ_0005526 in Interleukin-1β (IL-1β)-induced OA injury. Circ_0005526, microRNA-142-5p (miR-142-5p) or transcription factor 4 (TCF4) expression was measured via reverse transcription-quantitative polymerase chain reaction assay. Cell analysis was performed by Cell Counting Kit-8 assay for cell viability, EdU assay for cell proliferation and flow cytometry for cell apoptosis. The protein level detection was conducted using western blot. Target analysis was carried out via dual-luciferase reporter assay and RNA pull-down assay. Circ_0005526 was upregulated in OA cartilage tissues and IL-1β-exposed chondrocyte cells. IL-1β inhibited cell viability and proliferation but enhanced cell apoptosis and inflammation, then these damages were attenuated after downregulation of circ_0005526. Circ_0005526 interacted with miR-142-5p, and circ_0005526 knockdown suppressed IL-1β-induced OA progression through upregulating miR-142-5p. TCF4 was regulated by circ_0005526 via targeting miR-142-5p. The function of circ_0005526 was also achieved by upregulation of TCF4. These results unraveled that circ_0005526 promoted IL-1β-induced chondrocyte injury in OA via suppressing miR-142-5p binding to TCF4.

Cartilage Homeostasis and Osteoarthritis

“…CircRHOT1 enhances CCND1 expression by sponging miR-142-5p to inhibit chondrocyte autophagy and promote chondrocyte proliferation in OA [ 143 ]. Circ_0005526 promotes IL-1β-induced chondrocyte injury in OA by suppressing miR-142-5p binding to transcription factor 4 [ 144 ]. Circ_0043947 contributes to interleukin 1β-induced chondrocyte injury by sponging miR-671-5p to upregulate reticulon 3 expression [ 145 ].…”

Silencing of circ‐NT5C2 retards the progression of IL‐1β–induced osteoarthritis in an in vitro cell model by targeting the miR‐142‐5p/NAMPT axis

“…Moreover, several circRNAs, such as circ_0005526 and circ_SEC. 24A, can regulate OA progression in an OA cell model by sponging miR‐142‐5p 20,21 . Nonetheless, no studies proved the association of circ‐NT5C2 with miR‐142‐5p in OA.…”

“…17 Multiple studies have documented the protective role of miR-142-5p in OA. [18][19][20] Moreover, several cir-cRNAs, such as circ_0005526 and circ_SEC. 24A, can regulate OA progression in an OA cell model by sponging miR-142-5p.…”

“…24A, can regulate OA progression in an OA cell model by sponging miR-142-5p. 20,21 Nonetheless, no studies proved the association of circ-NT5C2 with miR-142-5p in OA. Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme of nicotinamide adenine dinucleotide (NAD), which plays a key role in redox processes.…”

“…Importantly, circRNAs have established a role as competing endogenous RNA (ceRNA) for miRNAs, thereby exerting crucial functions in the OA process 17 . Multiple studies have documented the protective role of miR‐142‐5p in OA 18–20 . Moreover, several circRNAs, such as circ_0005526 and circ_SEC.…”